Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish
Due to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In c...
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Wiley
2019-10-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.12727 |
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| author | Ti‐Lin Yi Meng‐Ting Pei Dai‐Qin Yang |
| author_facet | Ti‐Lin Yi Meng‐Ting Pei Dai‐Qin Yang |
| author_sort | Ti‐Lin Yi |
| collection | DOAJ |
| description | Due to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In contrast to mammals, fishes often possess more than one copy of the kiss and gpr54 genes. Our objectives were to identify all kiss/gpr54 genes in the genome of M. albus and to assess their involvement in sex reversal via their expression patterns (qPCR) in females, males, and intersex specimens. We identified only two genes: kiss2 and gpr54‐2. kiss2 expression was extremely high in the gonads of males, intermediate in females, and low in intersex; and reduced in all tissues of intersex. gpr54 expression was also extremely high in the gonads of males, high in intersex, but low in females. gpr54 expression in brain was high in all three sexes. In conclusion, (a) kiss1 has been functionally replaced in M. albus; (b) the functions of gpr54‐2 in brain are not sex‐specific; (c) kiss2 appears to undergo a ‘reset’ in the expression during the sex change; and (d) sex‐specific expression patterns in the gonads indicate that these two genes may play a role in sex reversal in fish. |
| format | Article |
| id | doaj-art-539660a2d50441febd67fc2ac6b1beb2 |
| institution | OA Journals |
| issn | 2211-5463 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-539660a2d50441febd67fc2ac6b1beb22025-08-20T02:19:22ZengWileyFEBS Open Bio2211-54632019-10-019101835184410.1002/2211-5463.12727Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fishTi‐Lin Yi0Meng‐Ting Pei1Dai‐Qin Yang2Yangtze University Engineering Research Center for Ecology and Agriculture Use of Wetland, Ministry of Education Jingzhou ChinaHubei Provincial Engineering and Technology Research Center for Monopterus albus Jingzhou ChinaYangtze University Engineering Research Center for Ecology and Agriculture Use of Wetland, Ministry of Education Jingzhou ChinaDue to its exceptionally small genome size and protogynous hermaphroditism, Monopterus albus has been proposed as a model for vertebrate sexual development. The Kiss/GPR54 system is a central regulator of sexual development in most vertebrates, but its role in sex reversal remains hypothetical. In contrast to mammals, fishes often possess more than one copy of the kiss and gpr54 genes. Our objectives were to identify all kiss/gpr54 genes in the genome of M. albus and to assess their involvement in sex reversal via their expression patterns (qPCR) in females, males, and intersex specimens. We identified only two genes: kiss2 and gpr54‐2. kiss2 expression was extremely high in the gonads of males, intermediate in females, and low in intersex; and reduced in all tissues of intersex. gpr54 expression was also extremely high in the gonads of males, high in intersex, but low in females. gpr54 expression in brain was high in all three sexes. In conclusion, (a) kiss1 has been functionally replaced in M. albus; (b) the functions of gpr54‐2 in brain are not sex‐specific; (c) kiss2 appears to undergo a ‘reset’ in the expression during the sex change; and (d) sex‐specific expression patterns in the gonads indicate that these two genes may play a role in sex reversal in fish.https://doi.org/10.1002/2211-5463.12727Asian swamp eelgene expressionHPG axisKisspeptin/GPR54 system |
| spellingShingle | Ti‐Lin Yi Meng‐Ting Pei Dai‐Qin Yang Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish FEBS Open Bio Asian swamp eel gene expression HPG axis Kisspeptin/GPR54 system |
| title | Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish |
| title_full | Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish |
| title_fullStr | Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish |
| title_full_unstemmed | Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish |
| title_short | Expression patterns of kiss2 and gpr54‐2 in Monopterus albus suggest these genes may play a role in sex reversal in fish |
| title_sort | expression patterns of kiss2 and gpr54 2 in monopterus albus suggest these genes may play a role in sex reversal in fish |
| topic | Asian swamp eel gene expression HPG axis Kisspeptin/GPR54 system |
| url | https://doi.org/10.1002/2211-5463.12727 |
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