The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma.
<h4>Objective</h4>The aim of the present study is to determine if CEACAM6 can be detected in the bile of patients with biliary cancer and can serve as a diagnostic biomarker for cholangiocarcinoma.<h4>Summary background data</h4>Distinguishing bile duct carcinoma from other d...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150195&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849722841851756544 |
|---|---|
| author | J Bart Rose Camilo Correa-Gallego Yu Li James Nelson Adnan Alseidi W Scott Helton Peter J Allen Michael I D'Angelica Ronald P DeMatteo Yuman Fong T Peter Kingham Kris V Kowdley William R Jarnagin Flavio G Rocha |
| author_facet | J Bart Rose Camilo Correa-Gallego Yu Li James Nelson Adnan Alseidi W Scott Helton Peter J Allen Michael I D'Angelica Ronald P DeMatteo Yuman Fong T Peter Kingham Kris V Kowdley William R Jarnagin Flavio G Rocha |
| author_sort | J Bart Rose |
| collection | DOAJ |
| description | <h4>Objective</h4>The aim of the present study is to determine if CEACAM6 can be detected in the bile of patients with biliary cancer and can serve as a diagnostic biomarker for cholangiocarcinoma.<h4>Summary background data</h4>Distinguishing bile duct carcinoma from other diagnoses is often difficult using endoscopic or percutaneous techniques. The cell surface protein CEACAM6 is over-expressed in many gastrointestinal cancers and may be selectively elevated in biliary adenocarcinoma.<h4>Methods</h4>Bile from patients with benign biliary disease and cholangiocarcinoma (hilar, intrahepatic and distal) was collected at the time of index operation. The concentration of CEACAM6 was quantified by sandwich enzyme-linked immunosorbent assay (ELISA) and correlated to pathologic diagnosis. Diagnostic capability of CEACAM6 was evaluated by Wilcoxon rank-sum, linear regression, multiple regression, and receiver operating characteristic (ROC) curve analysis.<h4>Results</h4>Bile from 83 patients was analyzed: 42 with benign disease and 41 with cholangiocarcinoma. Patients in the benign cohort were younger, predominantly female, and had lower median biliary CEACAM6 levels than patients in the malignant cohort (7.5 ng/ml vs. 40 ng/ml; p = <.001). ROC curve analysis determined CEACAM6 to be a positive predictor cholangiocarcinoma with a CEACAM6 level >14 ng/ml associated with 87.5% sensitivity, 69.1% specificity, and a likelihood ratio of 2.8 (AUC 0.74). Multiple regression analysis suggested elevated alkaline phosphatase and the presence of biliary endoprostheses may influence CEACAM6 levels.<h4>Conclusion</h4>Biliary CEACAM6 can identify patients with extrahepatic cholangiocarcinoma with a high degree of sensitivity and should be investigated further as a potential screening tool. |
| format | Article |
| id | doaj-art-5386bcbe9f784b00a00ebccff358b5d6 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-5386bcbe9f784b00a00ebccff358b5d62025-08-20T03:11:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015019510.1371/journal.pone.0150195The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma.J Bart RoseCamilo Correa-GallegoYu LiJames NelsonAdnan AlseidiW Scott HeltonPeter J AllenMichael I D'AngelicaRonald P DeMatteoYuman FongT Peter KinghamKris V KowdleyWilliam R JarnaginFlavio G Rocha<h4>Objective</h4>The aim of the present study is to determine if CEACAM6 can be detected in the bile of patients with biliary cancer and can serve as a diagnostic biomarker for cholangiocarcinoma.<h4>Summary background data</h4>Distinguishing bile duct carcinoma from other diagnoses is often difficult using endoscopic or percutaneous techniques. The cell surface protein CEACAM6 is over-expressed in many gastrointestinal cancers and may be selectively elevated in biliary adenocarcinoma.<h4>Methods</h4>Bile from patients with benign biliary disease and cholangiocarcinoma (hilar, intrahepatic and distal) was collected at the time of index operation. The concentration of CEACAM6 was quantified by sandwich enzyme-linked immunosorbent assay (ELISA) and correlated to pathologic diagnosis. Diagnostic capability of CEACAM6 was evaluated by Wilcoxon rank-sum, linear regression, multiple regression, and receiver operating characteristic (ROC) curve analysis.<h4>Results</h4>Bile from 83 patients was analyzed: 42 with benign disease and 41 with cholangiocarcinoma. Patients in the benign cohort were younger, predominantly female, and had lower median biliary CEACAM6 levels than patients in the malignant cohort (7.5 ng/ml vs. 40 ng/ml; p = <.001). ROC curve analysis determined CEACAM6 to be a positive predictor cholangiocarcinoma with a CEACAM6 level >14 ng/ml associated with 87.5% sensitivity, 69.1% specificity, and a likelihood ratio of 2.8 (AUC 0.74). Multiple regression analysis suggested elevated alkaline phosphatase and the presence of biliary endoprostheses may influence CEACAM6 levels.<h4>Conclusion</h4>Biliary CEACAM6 can identify patients with extrahepatic cholangiocarcinoma with a high degree of sensitivity and should be investigated further as a potential screening tool.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150195&type=printable |
| spellingShingle | J Bart Rose Camilo Correa-Gallego Yu Li James Nelson Adnan Alseidi W Scott Helton Peter J Allen Michael I D'Angelica Ronald P DeMatteo Yuman Fong T Peter Kingham Kris V Kowdley William R Jarnagin Flavio G Rocha The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma. PLoS ONE |
| title | The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma. |
| title_full | The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma. |
| title_fullStr | The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma. |
| title_full_unstemmed | The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma. |
| title_short | The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma. |
| title_sort | role of biliary carcinoembryonic antigen related cellular adhesion molecule 6 ceacam6 as a biomarker in cholangiocarcinoma |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150195&type=printable |
| work_keys_str_mv | AT jbartrose theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT camilocorreagallego theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT yuli theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT jamesnelson theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT adnanalseidi theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT wscotthelton theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT peterjallen theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT michaelidangelica theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT ronaldpdematteo theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT yumanfong theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT tpeterkingham theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT krisvkowdley theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT williamrjarnagin theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT flaviogrocha theroleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT jbartrose roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT camilocorreagallego roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT yuli roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT jamesnelson roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT adnanalseidi roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT wscotthelton roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT peterjallen roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT michaelidangelica roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT ronaldpdematteo roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT yumanfong roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT tpeterkingham roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT krisvkowdley roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT williamrjarnagin roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma AT flaviogrocha roleofbiliarycarcinoembryonicantigenrelatedcellularadhesionmolecule6ceacam6asabiomarkerincholangiocarcinoma |