Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsy

Abstract Gliomas are the most common primary tumors of the central nervous system, with epilepsy serving as a frequent clinical manifestation. Glioma-related epilepsy (GRE) significantly affects patients’ quality of life and prognosis. In this study, we integrated bioinformatics and multiple machine...

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Main Authors: Zhenpan Zhang, Jianhuang Huang, Caihou Lin, Risheng Liang
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-97333-4
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author Zhenpan Zhang
Jianhuang Huang
Caihou Lin
Risheng Liang
author_facet Zhenpan Zhang
Jianhuang Huang
Caihou Lin
Risheng Liang
author_sort Zhenpan Zhang
collection DOAJ
description Abstract Gliomas are the most common primary tumors of the central nervous system, with epilepsy serving as a frequent clinical manifestation. Glioma-related epilepsy (GRE) significantly affects patients’ quality of life and prognosis. In this study, we integrated bioinformatics and multiple machine learning methods to perform a proteomic analysis of brain tumor samples from patients with GRE and from those with gliomas none epilepsy (GNE). Our findings identified LY6H and GRM3 as potential signature proteins of GRE. Further investigation showed that LY6H and GRM3 expression levels were markedly reduced in GRE samples, with favorable diagnostic performance according to ROC curve analyses. Finally, we conducted an independent external validation using the Bluk-RNA dataset GSE199759, and the results corroborated our prior analyses. This work not only provides new biomarkers for the early detection of GRE but also offers valuable insights into its molecular mechanisms and potential therapeutic strategies.
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spelling doaj-art-5383fe48bbb945e2bf0a2621bc05fc382025-08-20T03:18:34ZengNature PortfolioScientific Reports2045-23222025-04-0115111410.1038/s41598-025-97333-4Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsyZhenpan Zhang0Jianhuang Huang1Caihou Lin2Risheng Liang3Department of Neurosurgery, Fujian Medical University Union HospitalDepartment of Neurosurgery, Affiliated Hospital of Putian UniversityDepartment of Neurosurgery, Fujian Medical University Union HospitalDepartment of Neurosurgery, Fujian Medical University Union HospitalAbstract Gliomas are the most common primary tumors of the central nervous system, with epilepsy serving as a frequent clinical manifestation. Glioma-related epilepsy (GRE) significantly affects patients’ quality of life and prognosis. In this study, we integrated bioinformatics and multiple machine learning methods to perform a proteomic analysis of brain tumor samples from patients with GRE and from those with gliomas none epilepsy (GNE). Our findings identified LY6H and GRM3 as potential signature proteins of GRE. Further investigation showed that LY6H and GRM3 expression levels were markedly reduced in GRE samples, with favorable diagnostic performance according to ROC curve analyses. Finally, we conducted an independent external validation using the Bluk-RNA dataset GSE199759, and the results corroborated our prior analyses. This work not only provides new biomarkers for the early detection of GRE but also offers valuable insights into its molecular mechanisms and potential therapeutic strategies.https://doi.org/10.1038/s41598-025-97333-4Glioma-related epilepsyProteomicBiomarkersLassoMachine learning
spellingShingle Zhenpan Zhang
Jianhuang Huang
Caihou Lin
Risheng Liang
Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsy
Scientific Reports
Glioma-related epilepsy
Proteomic
Biomarkers
Lasso
Machine learning
title Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsy
title_full Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsy
title_fullStr Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsy
title_full_unstemmed Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsy
title_short Identification and validation of LY6H and GRM3 as candidate biomarkers for Glioma-related epilepsy
title_sort identification and validation of ly6h and grm3 as candidate biomarkers for glioma related epilepsy
topic Glioma-related epilepsy
Proteomic
Biomarkers
Lasso
Machine learning
url https://doi.org/10.1038/s41598-025-97333-4
work_keys_str_mv AT zhenpanzhang identificationandvalidationofly6handgrm3ascandidatebiomarkersforgliomarelatedepilepsy
AT jianhuanghuang identificationandvalidationofly6handgrm3ascandidatebiomarkersforgliomarelatedepilepsy
AT caihoulin identificationandvalidationofly6handgrm3ascandidatebiomarkersforgliomarelatedepilepsy
AT rishengliang identificationandvalidationofly6handgrm3ascandidatebiomarkersforgliomarelatedepilepsy