Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain Health
The efficacy of Cuban sugarcane-extracted policosanol (Raydel<sup>®</sup>), a purified blend of eight long-chain aliphatic alcohols, was compared to copycat sugarcane-extract powder (SCEP) to assess their effects on dyslipidemia, oxidative stress, and vital organs of zebrafish under the...
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2025-05-01
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| author | Kyung-Hyun Cho Ashutosh Bahuguna Sang Hyuk Lee Ji-Eun Kim Yunki Lee Cheolmin Jeon |
| author_facet | Kyung-Hyun Cho Ashutosh Bahuguna Sang Hyuk Lee Ji-Eun Kim Yunki Lee Cheolmin Jeon |
| author_sort | Kyung-Hyun Cho |
| collection | DOAJ |
| description | The efficacy of Cuban sugarcane-extracted policosanol (Raydel<sup>®</sup>), a purified blend of eight long-chain aliphatic alcohols, was compared to copycat sugarcane-extract powder (SCEP) to assess their effects on dyslipidemia, oxidative stress, and vital organs of zebrafish under the influence of a high-cholesterol diet (HCD). Zebrafish were fed with HCD (final 4%, <i>w</i>/<i>w</i>) or HCD infused with policosanol (PCO, final 1%, <i>w</i>/<i>w</i>) or SCEP (final 1%, <i>w</i>/<i>w</i>). Post 14-week consumption, blood and organs were harvested and processed for various biochemical, histological, and immunohistochemical (IHC) examinations, and fluorescent staining. Following 14-week consumption, the PCO-supplemented group exhibited higher zebrafish survival probability than the SCEP-supplemented group. Both PCO and SCEP substantially impacted the HCD-disrupted plasma lipid profile; however, PCO supplementation exhibited a significantly better effect than SCEP. Similarly, PCO supplementation significantly improved the blood glucose level, hepatic function biomarkers, and oxidative-antioxidant balance disturbed by HCD. PCO supplementation displayed a substantial inhibitory effect against HCD-induced fatty liver changes, nephromegaly, and cellular senescence. Likewise, PCO effectively protected the brain against HCD-induced apoptosis and accumulation of 4-hydroxynonenal (4-HNE); in contrast, SCEP supplementation showed almost no effect in reducing such adverse changes. The comparative findings between PCO and SCEP highlight the protective effects of PCO against HCD-induced oxidative stress and dyslipidemia via the enhancement of antioxidant markers, leading to protection of the liver, kidney, and brain, while SCEP failed to achieve similar outcomes. |
| format | Article |
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| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-53832473a8ff4ca0bc708ff34613cce22025-08-20T03:47:52ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-05-0147535410.3390/cimb47050354Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain HealthKyung-Hyun Cho0Ashutosh Bahuguna1Sang Hyuk Lee2Ji-Eun Kim3Yunki Lee4Cheolmin Jeon5Raydel Research Institute, Medical Innovation Complex, Daegu 41061, Republic of KoreaRaydel Research Institute, Medical Innovation Complex, Daegu 41061, Republic of KoreaRaydel Research Institute, Medical Innovation Complex, Daegu 41061, Republic of KoreaRaydel Research Institute, Medical Innovation Complex, Daegu 41061, Republic of KoreaRaydel Research Institute, Medical Innovation Complex, Daegu 41061, Republic of KoreaRaydel Research Institute, Medical Innovation Complex, Daegu 41061, Republic of KoreaThe efficacy of Cuban sugarcane-extracted policosanol (Raydel<sup>®</sup>), a purified blend of eight long-chain aliphatic alcohols, was compared to copycat sugarcane-extract powder (SCEP) to assess their effects on dyslipidemia, oxidative stress, and vital organs of zebrafish under the influence of a high-cholesterol diet (HCD). Zebrafish were fed with HCD (final 4%, <i>w</i>/<i>w</i>) or HCD infused with policosanol (PCO, final 1%, <i>w</i>/<i>w</i>) or SCEP (final 1%, <i>w</i>/<i>w</i>). Post 14-week consumption, blood and organs were harvested and processed for various biochemical, histological, and immunohistochemical (IHC) examinations, and fluorescent staining. Following 14-week consumption, the PCO-supplemented group exhibited higher zebrafish survival probability than the SCEP-supplemented group. Both PCO and SCEP substantially impacted the HCD-disrupted plasma lipid profile; however, PCO supplementation exhibited a significantly better effect than SCEP. Similarly, PCO supplementation significantly improved the blood glucose level, hepatic function biomarkers, and oxidative-antioxidant balance disturbed by HCD. PCO supplementation displayed a substantial inhibitory effect against HCD-induced fatty liver changes, nephromegaly, and cellular senescence. Likewise, PCO effectively protected the brain against HCD-induced apoptosis and accumulation of 4-hydroxynonenal (4-HNE); in contrast, SCEP supplementation showed almost no effect in reducing such adverse changes. The comparative findings between PCO and SCEP highlight the protective effects of PCO against HCD-induced oxidative stress and dyslipidemia via the enhancement of antioxidant markers, leading to protection of the liver, kidney, and brain, while SCEP failed to achieve similar outcomes.https://www.mdpi.com/1467-3045/47/5/354dyslipidemiafatty liverglucose4-hydroxynonenalinterleukin 6policosanol |
| spellingShingle | Kyung-Hyun Cho Ashutosh Bahuguna Sang Hyuk Lee Ji-Eun Kim Yunki Lee Cheolmin Jeon Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain Health Current Issues in Molecular Biology dyslipidemia fatty liver glucose 4-hydroxynonenal interleukin 6 policosanol |
| title | Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain Health |
| title_full | Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain Health |
| title_fullStr | Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain Health |
| title_full_unstemmed | Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain Health |
| title_short | Effect of 14-Week Supplementation of Highly Purified Policosanol (Raydel<sup>®</sup>) and a Sugar Cane Extract Powder (SCEP) on Dyslipidemia and Oxidative Variables in Hyperlipidemic Zebrafish: Insight into Liver, Kidney, and Brain Health |
| title_sort | effect of 14 week supplementation of highly purified policosanol raydel sup r sup and a sugar cane extract powder scep on dyslipidemia and oxidative variables in hyperlipidemic zebrafish insight into liver kidney and brain health |
| topic | dyslipidemia fatty liver glucose 4-hydroxynonenal interleukin 6 policosanol |
| url | https://www.mdpi.com/1467-3045/47/5/354 |
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