Development and validation of a RP-HPLC method for the quantisation studies of metronidazole and furazolidone from product Enteroguard M
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of metronidazole and furazolidone in tablet dosage forms of ENTEROGUARD M. HPLC separation was carried out by reversed phase chromatography Kromasil C18 (250mm x 4,6mm i.d.; 5μm particle...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Asociaţia Naţională a Fabricanţilor de Produse de Uz Veterinar
2010-12-01
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| Series: | Medicamentul Veterinar |
| Subjects: | |
| Online Access: | http://www.veterinarypharmacon.com/docs/610-2010-%20ART.7.pdf |
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| Summary: | An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of metronidazole and furazolidone in tablet dosage forms of ENTEROGUARD M. HPLC separation was carried out by reversed phase chromatography Kromasil C18 (250mm x 4,6mm i.d.; 5μm particle size), held at 25°C. The mobile phase consisted of methanol/ 0,1% phosphoric acid aq. (20/80v/v), run at flow rate of 1 mL/ min and with UV detection at 317nm. Method validation investigated parameters such as linearity (r2 = 0.9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of ENTEROGUARD M tablets. |
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| ISSN: | 1843-9527 2069-2463 |