Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neurons
Abstract The activity of the telencephalon is shaped by pallial and subpallial GABAergic neurons, two large populations produced in the embryonic ganglionic eminence. However, knowledge about the fate specification of neuron subtypes is limited, especially whether there is a common mechanism directi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60338-8 |
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| author | Lijuan Sun Feng Xiong Feier Huang Shuangshuang Dong Pei Zhu Pengfei Jiang Baoshen Zhang Xinxin Zhang Jie Sun Hanchuan Peng Chunjie Zhao |
| author_facet | Lijuan Sun Feng Xiong Feier Huang Shuangshuang Dong Pei Zhu Pengfei Jiang Baoshen Zhang Xinxin Zhang Jie Sun Hanchuan Peng Chunjie Zhao |
| author_sort | Lijuan Sun |
| collection | DOAJ |
| description | Abstract The activity of the telencephalon is shaped by pallial and subpallial GABAergic neurons, two large populations produced in the embryonic ganglionic eminence. However, knowledge about the fate specification of neuron subtypes is limited, especially whether there is a common mechanism directing the fate choice of pallial versus subpallial populations remains unknown, largely because each population comprises numerous subtypes. Here, using sc-RNA sequencing combined with loss-of-function we profile ganglion eminence lineages and find that Foxg1 deletion causes the pallial population to adopt subpallial fates in mice. We delineate developmental trajectories and reveal FOXG1-driven transcriptional programs that specify neuron subtypes in each GE lineage and transcription factors that direct lineage bifurcation decisions. We uncover a common mechanism that drives pallial fate over subpallial fate across ganglion eminence lineages. Our study illuminates the control of production between pallial and subpallial populations and offers transcriptomic insights into the pathogenesis of GABAergic neuron-related disorders. |
| format | Article |
| id | doaj-art-537be55fc0a14233a09a943d23a3a8e8 |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-537be55fc0a14233a09a943d23a3a8e82025-08-20T03:22:03ZengNature PortfolioNature Communications2041-17232025-05-0116112010.1038/s41467-025-60338-8Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neuronsLijuan Sun0Feng Xiong1Feier Huang2Shuangshuang Dong3Pei Zhu4Pengfei Jiang5Baoshen Zhang6Xinxin Zhang7Jie Sun8Hanchuan Peng9Chunjie Zhao10Key Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityNew Cornerstone Science Laboratory, Institute for Brain and Intelligence, Southeast UniversityKey Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityKey Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityKey Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityKey Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityKey Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityKey Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityDepartment of Anesthesiology, Zhongda hospital, Southeast UniversityNew Cornerstone Science Laboratory, Institute for Brain and Intelligence, Southeast UniversityKey Laboratory of Developmental Genes and Human Diseases, Ministry of Education, School of Medicine, Southeast UniversityAbstract The activity of the telencephalon is shaped by pallial and subpallial GABAergic neurons, two large populations produced in the embryonic ganglionic eminence. However, knowledge about the fate specification of neuron subtypes is limited, especially whether there is a common mechanism directing the fate choice of pallial versus subpallial populations remains unknown, largely because each population comprises numerous subtypes. Here, using sc-RNA sequencing combined with loss-of-function we profile ganglion eminence lineages and find that Foxg1 deletion causes the pallial population to adopt subpallial fates in mice. We delineate developmental trajectories and reveal FOXG1-driven transcriptional programs that specify neuron subtypes in each GE lineage and transcription factors that direct lineage bifurcation decisions. We uncover a common mechanism that drives pallial fate over subpallial fate across ganglion eminence lineages. Our study illuminates the control of production between pallial and subpallial populations and offers transcriptomic insights into the pathogenesis of GABAergic neuron-related disorders.https://doi.org/10.1038/s41467-025-60338-8 |
| spellingShingle | Lijuan Sun Feng Xiong Feier Huang Shuangshuang Dong Pei Zhu Pengfei Jiang Baoshen Zhang Xinxin Zhang Jie Sun Hanchuan Peng Chunjie Zhao Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neurons Nature Communications |
| title | Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neurons |
| title_full | Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neurons |
| title_fullStr | Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neurons |
| title_full_unstemmed | Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neurons |
| title_short | Transcriptomic insights into fate choice of pallial versus subpallial GABAergic neurons |
| title_sort | transcriptomic insights into fate choice of pallial versus subpallial gabaergic neurons |
| url | https://doi.org/10.1038/s41467-025-60338-8 |
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