Bioenergetic stress potentiates antimicrobial resistance and persistence
Abstract The bactericidal action of some antibiotics is associated with increased ATP consumption, cellular respiration, and reactive oxygen species (ROS) formation. Here, we investigate the effects of ‘bioenergetic stress’, induced by constitutive hydrolysis of ATP and NADH, on antibiotic efficacy...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60302-6 |
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| author | Barry Li Shivani Srivastava Mustafa Shaikh Gautam Mereddy Madison R. Garcia Eric N. Chiles Avi Shah Boatema Ofori-Anyinam Ting-Yu Chu Nicole J. Cheney Douglas McCloskey Xiaoyang Su Jason H. Yang |
| author_facet | Barry Li Shivani Srivastava Mustafa Shaikh Gautam Mereddy Madison R. Garcia Eric N. Chiles Avi Shah Boatema Ofori-Anyinam Ting-Yu Chu Nicole J. Cheney Douglas McCloskey Xiaoyang Su Jason H. Yang |
| author_sort | Barry Li |
| collection | DOAJ |
| description | Abstract The bactericidal action of some antibiotics is associated with increased ATP consumption, cellular respiration, and reactive oxygen species (ROS) formation. Here, we investigate the effects of ‘bioenergetic stress’, induced by constitutive hydrolysis of ATP and NADH, on antibiotic efficacy in Escherichia coli. We show that bioenergetic stress potentiates the evolution of antibiotic resistance via enhanced ROS production, mutagenic break repair, and transcription-coupled repair. In addition, bioenergetic stress potentiates antibiotic persistence via the stringent response. We propose a model in which the balance between ATP consumption versus production regulates antibiotic resistance and persistence. |
| format | Article |
| id | doaj-art-53694ab169564ac4bbf2bbcb6a6aed3d |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-53694ab169564ac4bbf2bbcb6a6aed3d2025-08-20T03:45:32ZengNature PortfolioNature Communications2041-17232025-06-0116111810.1038/s41467-025-60302-6Bioenergetic stress potentiates antimicrobial resistance and persistenceBarry Li0Shivani Srivastava1Mustafa Shaikh2Gautam Mereddy3Madison R. Garcia4Eric N. Chiles5Avi Shah6Boatema Ofori-Anyinam7Ting-Yu Chu8Nicole J. Cheney9Douglas McCloskey10Xiaoyang Su11Jason H. Yang12Center for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolRutgers Cancer Institute of New Jersey; Rutgers University, The State University of New JerseyCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolNovo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkRutgers Cancer Institute of New Jersey; Rutgers University, The State University of New JerseyCenter for Emerging and Re-emerging Pathogens, Rutgers New Jersey Medical SchoolAbstract The bactericidal action of some antibiotics is associated with increased ATP consumption, cellular respiration, and reactive oxygen species (ROS) formation. Here, we investigate the effects of ‘bioenergetic stress’, induced by constitutive hydrolysis of ATP and NADH, on antibiotic efficacy in Escherichia coli. We show that bioenergetic stress potentiates the evolution of antibiotic resistance via enhanced ROS production, mutagenic break repair, and transcription-coupled repair. In addition, bioenergetic stress potentiates antibiotic persistence via the stringent response. We propose a model in which the balance between ATP consumption versus production regulates antibiotic resistance and persistence.https://doi.org/10.1038/s41467-025-60302-6 |
| spellingShingle | Barry Li Shivani Srivastava Mustafa Shaikh Gautam Mereddy Madison R. Garcia Eric N. Chiles Avi Shah Boatema Ofori-Anyinam Ting-Yu Chu Nicole J. Cheney Douglas McCloskey Xiaoyang Su Jason H. Yang Bioenergetic stress potentiates antimicrobial resistance and persistence Nature Communications |
| title | Bioenergetic stress potentiates antimicrobial resistance and persistence |
| title_full | Bioenergetic stress potentiates antimicrobial resistance and persistence |
| title_fullStr | Bioenergetic stress potentiates antimicrobial resistance and persistence |
| title_full_unstemmed | Bioenergetic stress potentiates antimicrobial resistance and persistence |
| title_short | Bioenergetic stress potentiates antimicrobial resistance and persistence |
| title_sort | bioenergetic stress potentiates antimicrobial resistance and persistence |
| url | https://doi.org/10.1038/s41467-025-60302-6 |
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