The role of Pnut and its functional domains in Drosophila spermatogenesis
Drosophila Pnut protein belongs to the family of septins, conservative GTPases participating in cytokinesis and many more other fundamental cellular processes. Because of their filamentous appearance, membrane association and functions, septins are considered as the fourth component of the cytoskele...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2016-03-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/526 |
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| author | K. A. Akhmetova N. D. Dorogova E. U. Bolobolova I. N. Chesnokov S. A. Fedorova |
| author_facet | K. A. Akhmetova N. D. Dorogova E. U. Bolobolova I. N. Chesnokov S. A. Fedorova |
| author_sort | K. A. Akhmetova |
| collection | DOAJ |
| description | Drosophila Pnut protein belongs to the family of septins, conservative GTPases participating in cytokinesis and many more other fundamental cellular processes. Because of their filamentous appearance, membrane association and functions, septins are considered as the fourth component of the cytoskeleton, along with actin, microtubules and intermediate filaments. However, septins are much less studied than the other cytoskeleton elements. We had previously demonstrated that deletion of the peanut (pnut) gene leads to mitotic abnormalities in somatic cells. The goal of this work was to study the role of pnut in Drosophila spermatogenesis. We designed a construct for pnut RNA interference allowing pnut expression to be suppressed ectopically. We analyzed the effect of pnut RNA interference on Drosophila spermatogenesis. The most sensitive to Pnut depletion were germ line cells at the earliest stages of spermatogenesis: the suppression of pnut expression at these stages leads to male sterility as a result of immotile sperm. Testes of those sterile males did not show any significant meiotic defects; axonemes and mitochondria were normal. We also analyzed the effect of mutations in Pnut conservative domains on Drosophila spermatogenesis. Mutations in the GTPase domain resulted in cyst elongation defects. Deletions of the C-terminal domain led to abnormal testis morphology. Both GTPase domain and C-terminal domain mutant males were sterile and produced immotile sperm. To summarize, we showed that Pnut participates in spermiogenesis, that is, late stages of spermatogenesis, when major morphological changes in spermatocytes occur. |
| format | Article |
| id | doaj-art-5365cca7628543e997f729ca39aa2c47 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-5365cca7628543e997f729ca39aa2c472025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592016-03-01201657110.18699/VJ16.104447The role of Pnut and its functional domains in Drosophila spermatogenesisK. A. Akhmetova0N. D. Dorogova1E. U. Bolobolova2I. N. Chesnokov3S. A. Fedorova4Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia University of Alabama at Birmingham, Birmingham, USAInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaUniversity of Alabama at Birmingham, Birmingham, USAInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, RussiaDrosophila Pnut protein belongs to the family of septins, conservative GTPases participating in cytokinesis and many more other fundamental cellular processes. Because of their filamentous appearance, membrane association and functions, septins are considered as the fourth component of the cytoskeleton, along with actin, microtubules and intermediate filaments. However, septins are much less studied than the other cytoskeleton elements. We had previously demonstrated that deletion of the peanut (pnut) gene leads to mitotic abnormalities in somatic cells. The goal of this work was to study the role of pnut in Drosophila spermatogenesis. We designed a construct for pnut RNA interference allowing pnut expression to be suppressed ectopically. We analyzed the effect of pnut RNA interference on Drosophila spermatogenesis. The most sensitive to Pnut depletion were germ line cells at the earliest stages of spermatogenesis: the suppression of pnut expression at these stages leads to male sterility as a result of immotile sperm. Testes of those sterile males did not show any significant meiotic defects; axonemes and mitochondria were normal. We also analyzed the effect of mutations in Pnut conservative domains on Drosophila spermatogenesis. Mutations in the GTPase domain resulted in cyst elongation defects. Deletions of the C-terminal domain led to abnormal testis morphology. Both GTPase domain and C-terminal domain mutant males were sterile and produced immotile sperm. To summarize, we showed that Pnut participates in spermiogenesis, that is, late stages of spermatogenesis, when major morphological changes in spermatocytes occur.https://vavilov.elpub.ru/jour/article/view/526drosophilarna interferencespermatogenesisseptinpeanutpnut |
| spellingShingle | K. A. Akhmetova N. D. Dorogova E. U. Bolobolova I. N. Chesnokov S. A. Fedorova The role of Pnut and its functional domains in Drosophila spermatogenesis Вавиловский журнал генетики и селекции drosophila rna interference spermatogenesis septin peanut pnut |
| title | The role of Pnut and its functional domains in Drosophila spermatogenesis |
| title_full | The role of Pnut and its functional domains in Drosophila spermatogenesis |
| title_fullStr | The role of Pnut and its functional domains in Drosophila spermatogenesis |
| title_full_unstemmed | The role of Pnut and its functional domains in Drosophila spermatogenesis |
| title_short | The role of Pnut and its functional domains in Drosophila spermatogenesis |
| title_sort | role of pnut and its functional domains in drosophila spermatogenesis |
| topic | drosophila rna interference spermatogenesis septin peanut pnut |
| url | https://vavilov.elpub.ru/jour/article/view/526 |
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