Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice
Objective(s): The use of cryoablation for colorectal liver metastases (CLM) remains limited and controversial. This study aimed to investigate the antitumor immune response following cryoablation combined with granulocyte-macrophage colony-stimulating factor (GM-CSF) treatment in a CLM mouse model.M...
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Mashhad University of Medical Sciences
2025-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_25490_64d22c68f08eb1164262ec235d2aaead.pdf |
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| author | Junfeng Wang Dalu Kong |
| author_facet | Junfeng Wang Dalu Kong |
| author_sort | Junfeng Wang |
| collection | DOAJ |
| description | Objective(s): The use of cryoablation for colorectal liver metastases (CLM) remains limited and controversial. This study aimed to investigate the antitumor immune response following cryoablation combined with granulocyte-macrophage colony-stimulating factor (GM-CSF) treatment in a CLM mouse model.Materials and Methods: A CLM mouse model was established using BALB/c mice. The tumor-bearing mice were randomly divided into Control group, GM-CSF group, cryoablation group, and cryoablation + GM-CSF group. Tumor size, survival time, dendritic cells (DCs) count, serum cytokine levels (IL-4, IFN-γ), and the Th1/Th2 ratio (IFN-γ/IL-4) were compared among the four groups.Results: The combination of cryoablation and GM-CSF demonstrated synergistic effects, resulting in the smallest tumor lesion, longest mean survival time, and highest DC count on day 21 post-treatment compared to other groups. Both cryoablation alone and combined with GM-CSF significantly increased serum IFN-γ levels and suppressed IL-4 levels on day 21 compared to pre-treatment levels (P<0.05). Notably, the combination of cryoablation and GM-CSF significantly elevated the Th1/Th2 ratio (P<0.05).Conclusion: Combining cryoablation with GM-CSF treatment holds promise for CLM management. It exhibits increased DC infiltration within the tumor microenvironment, enhanced immune responses, and prolonged survival in tumor-bearing mice. |
| format | Article |
| id | doaj-art-53643ea17e8a49aa85ec845ed76d8c8c |
| institution | DOAJ |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Mashhad University of Medical Sciences |
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| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-53643ea17e8a49aa85ec845ed76d8c8c2025-08-20T03:08:50ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-05-0128558459110.22038/ijbms.2025.82910.1798025490Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing miceJunfeng Wang0Dalu Kong1Department of Colorectal Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, ChinaDepartment of Colorectal Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, ChinaObjective(s): The use of cryoablation for colorectal liver metastases (CLM) remains limited and controversial. This study aimed to investigate the antitumor immune response following cryoablation combined with granulocyte-macrophage colony-stimulating factor (GM-CSF) treatment in a CLM mouse model.Materials and Methods: A CLM mouse model was established using BALB/c mice. The tumor-bearing mice were randomly divided into Control group, GM-CSF group, cryoablation group, and cryoablation + GM-CSF group. Tumor size, survival time, dendritic cells (DCs) count, serum cytokine levels (IL-4, IFN-γ), and the Th1/Th2 ratio (IFN-γ/IL-4) were compared among the four groups.Results: The combination of cryoablation and GM-CSF demonstrated synergistic effects, resulting in the smallest tumor lesion, longest mean survival time, and highest DC count on day 21 post-treatment compared to other groups. Both cryoablation alone and combined with GM-CSF significantly increased serum IFN-γ levels and suppressed IL-4 levels on day 21 compared to pre-treatment levels (P<0.05). Notably, the combination of cryoablation and GM-CSF significantly elevated the Th1/Th2 ratio (P<0.05).Conclusion: Combining cryoablation with GM-CSF treatment holds promise for CLM management. It exhibits increased DC infiltration within the tumor microenvironment, enhanced immune responses, and prolonged survival in tumor-bearing mice.https://ijbms.mums.ac.ir/article_25490_64d22c68f08eb1164262ec235d2aaead.pdfcolorectal neoplasmscryosurgerycytokinesdendritic cellsgranulocyte-macrophage - colony-stimulating factorimmunityliver neoplasmstumor microenvironment |
| spellingShingle | Junfeng Wang Dalu Kong Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice Iranian Journal of Basic Medical Sciences colorectal neoplasms cryosurgery cytokines dendritic cells granulocyte-macrophage - colony-stimulating factor immunity liver neoplasms tumor microenvironment |
| title | Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice |
| title_full | Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice |
| title_fullStr | Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice |
| title_full_unstemmed | Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice |
| title_short | Synergistic effects of cryoablation and GM-CSF in colorectal liver metastases management in tumor-bearing mice |
| title_sort | synergistic effects of cryoablation and gm csf in colorectal liver metastases management in tumor bearing mice |
| topic | colorectal neoplasms cryosurgery cytokines dendritic cells granulocyte-macrophage - colony-stimulating factor immunity liver neoplasms tumor microenvironment |
| url | https://ijbms.mums.ac.ir/article_25490_64d22c68f08eb1164262ec235d2aaead.pdf |
| work_keys_str_mv | AT junfengwang synergisticeffectsofcryoablationandgmcsfincolorectallivermetastasesmanagementintumorbearingmice AT dalukong synergisticeffectsofcryoablationandgmcsfincolorectallivermetastasesmanagementintumorbearingmice |