Preparing thin cross sections of Arabidopsis roots without embedding

Here, we describe a method for obtaining thin cross sections of Arabidopsis thaliana roots without fixation and embedding. Roots were grown in pinholes made in a solidified growth medium, and cross sections were prepared without pretreatment. Using this method, we detected unique distributions of tw...

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Bibliographic Details
Main Authors: Naoyuki Sotta, Toru Fujiwara
Format: Article
Language:English
Published: Taylor & Francis Group 2017-12-01
Series:BioTechniques
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Online Access:https://www.future-science.com/doi/10.2144/000114621
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