Preparing thin cross sections of Arabidopsis roots without embedding
Here, we describe a method for obtaining thin cross sections of Arabidopsis thaliana roots without fixation and embedding. Roots were grown in pinholes made in a solidified growth medium, and cross sections were prepared without pretreatment. Using this method, we detected unique distributions of tw...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-12-01
|
| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114621 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Here, we describe a method for obtaining thin cross sections of Arabidopsis thaliana roots without fixation and embedding. Roots were grown in pinholes made in a solidified growth medium, and cross sections were prepared without pretreatment. Using this method, we detected unique distributions of two polar-localized proteins—green fluorescent protein (GFP)-tagged BOR1 and NIP5;1—with less sample preparation time than conventional methods. This method is simple, rapid, and yields high-quality cross-section images that are free from artifacts commonly associated with embedding or the sample preparation procedures used in many conventional methods. |
|---|---|
| ISSN: | 0736-6205 1940-9818 |