The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation study
Objective: To investigate the potential targets and mechanisms of Draconis Sanguis (DS), a valuable traditional Chinese medicine derived from the resin of the palm tree Daemonorops draco Bl (D. Sanguis, Xue Jie), in the treatment of myocardial infarction (MI). Methods: We explored the potential mech...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754825000547 |
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| author | Keyan Wang Rongxin Zhu Junjun Li Binhua Yuan Xiang Li Yunlin Li Mingyue Huang Fangfang Rui Chun Li Wei Wang |
| author_facet | Keyan Wang Rongxin Zhu Junjun Li Binhua Yuan Xiang Li Yunlin Li Mingyue Huang Fangfang Rui Chun Li Wei Wang |
| author_sort | Keyan Wang |
| collection | DOAJ |
| description | Objective: To investigate the potential targets and mechanisms of Draconis Sanguis (DS), a valuable traditional Chinese medicine derived from the resin of the palm tree Daemonorops draco Bl (D. Sanguis, Xue Jie), in the treatment of myocardial infarction (MI). Methods: We explored the potential mechanisms of DS in the treatment of MI using network pharmacology, bioinformatic techniques, and transcriptomic analysis, followed by validation through in vivo and in vitro experiments. Results: Network pharmacology and bioinformatic analyses identified five genes (Fpr1, Glul, Mme, Mmp9, and Pla2g7) as potential targets for MI treatment. Moreover, DS significantly ameliorated cardiac function, inflammatory responses, and MI-induced myocardial fibrosis in vivo. Transcriptomic and bioinformatic analyses identified Pla2g7 as the most critical target in the DS treatment of MI. Molecular docking revealed that the key active ingredient in DS has a strong affinity for this gene. Furthermore, DS reduced the expression of Pla2g7 (P = .0009), NLRP3 (P = .003), interleukin-18 (P < .001), and interleukin-1β (P = .004) mRNAs in vivo. Conclusions: The results indicate that DS can downregulate the expression of Pla2g7 and reduce the inflammatory response. This demonstrates the potential therapeutic target of DS and the mechanism underlying its cardioprotective effects. |
| format | Article |
| id | doaj-art-5343b024b01d4c6ca110d20d132c2d44 |
| institution | Kabale University |
| issn | 2095-7548 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj-art-5343b024b01d4c6ca110d20d132c2d442025-08-20T03:58:36ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482025-07-0112333634710.1016/j.jtcms.2025.05.004The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation studyKeyan Wang0Rongxin Zhu1Junjun Li2Binhua Yuan3Xiang Li4Yunlin Li5Mingyue Huang6Fangfang Rui7Chun Li8Wei Wang9School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, ChinaBeijing Key Laboratory of Traditional Chinese Medicine Syndrome and Formula, Beijing University of Chinese Medicine, Beijing 102488, China; Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China; Corresponding authors.State Key Laboratory of Traditional Chinese Medicine Syndrome, Guangzhou University of Chinese Medicine, Guangzhou 510006, China; Corresponding authors.Objective: To investigate the potential targets and mechanisms of Draconis Sanguis (DS), a valuable traditional Chinese medicine derived from the resin of the palm tree Daemonorops draco Bl (D. Sanguis, Xue Jie), in the treatment of myocardial infarction (MI). Methods: We explored the potential mechanisms of DS in the treatment of MI using network pharmacology, bioinformatic techniques, and transcriptomic analysis, followed by validation through in vivo and in vitro experiments. Results: Network pharmacology and bioinformatic analyses identified five genes (Fpr1, Glul, Mme, Mmp9, and Pla2g7) as potential targets for MI treatment. Moreover, DS significantly ameliorated cardiac function, inflammatory responses, and MI-induced myocardial fibrosis in vivo. Transcriptomic and bioinformatic analyses identified Pla2g7 as the most critical target in the DS treatment of MI. Molecular docking revealed that the key active ingredient in DS has a strong affinity for this gene. Furthermore, DS reduced the expression of Pla2g7 (P = .0009), NLRP3 (P = .003), interleukin-18 (P < .001), and interleukin-1β (P = .004) mRNAs in vivo. Conclusions: The results indicate that DS can downregulate the expression of Pla2g7 and reduce the inflammatory response. This demonstrates the potential therapeutic target of DS and the mechanism underlying its cardioprotective effects.http://www.sciencedirect.com/science/article/pii/S2095754825000547Myocardial infarctionDraconis sanguisNetwork pharmacologyBioinformaticsRNA-seqPla2g7 |
| spellingShingle | Keyan Wang Rongxin Zhu Junjun Li Binhua Yuan Xiang Li Yunlin Li Mingyue Huang Fangfang Rui Chun Li Wei Wang The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation study Journal of Traditional Chinese Medical Sciences Myocardial infarction Draconis sanguis Network pharmacology Bioinformatics RNA-seq Pla2g7 |
| title | The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation study |
| title_full | The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation study |
| title_fullStr | The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation study |
| title_full_unstemmed | The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation study |
| title_short | The cardioprotective mechanisms of draconis sanguis: An integrated network pharmacology, bioinformatics, and experimental validation study |
| title_sort | cardioprotective mechanisms of draconis sanguis an integrated network pharmacology bioinformatics and experimental validation study |
| topic | Myocardial infarction Draconis sanguis Network pharmacology Bioinformatics RNA-seq Pla2g7 |
| url | http://www.sciencedirect.com/science/article/pii/S2095754825000547 |
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