Population genetics of jaguarundis in Mexico: Implications for future research and conservation

Abstract The jaguarundi (Puma yagouaroundi) is a Neotropical felid that ranges from northern Mexico to South America. The population trend for jaguarundis is declining, yet much remains unknown about their ecology. We live‐trapped 11 jaguarundis during 1991–2004 in Tamaulipas, Mexico, and we collect...

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Main Authors: Joseph D. Holbrook, Arturo Caso, Randy W. Deyoung, Michael E. Tewes
Format: Article
Language:English
Published: Wiley 2013-06-01
Series:Wildlife Society Bulletin
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Online Access:https://doi.org/10.1002/wsb.246
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author Joseph D. Holbrook
Arturo Caso
Randy W. Deyoung
Michael E. Tewes
author_facet Joseph D. Holbrook
Arturo Caso
Randy W. Deyoung
Michael E. Tewes
author_sort Joseph D. Holbrook
collection DOAJ
description Abstract The jaguarundi (Puma yagouaroundi) is a Neotropical felid that ranges from northern Mexico to South America. The population trend for jaguarundis is declining, yet much remains unknown about their ecology. We live‐trapped 11 jaguarundis during 1991–2004 in Tamaulipas, Mexico, and we collected blood for genetic analyses. Our objectives were to 1) estimate neutral diversity using microsatellite and mitochondrial (mtDNA) markers, 2) estimate potentially adaptive diversity in coat coloration via the melanocortin‐1 receptor gene (MC1R), and 3) provide recommendations on the use of genetic markers for noninvasive studies. We observed moderate levels of heterozygosity (HE = 0.49, SD = 0.22) and number of alleles (A = 4.00, SD = 1.65) at 12 feline microsatellite loci. The probability of identifying 2 different jaguarundis as the same individual was low using only 4–7 loci (P(ID) < 0.001 and P(ID)sib < 0.01, respectively). We observed one mtDNA haplotype, indicating no mtDNA diversity. However, we documented diversity with the MC1R assay. The frequency of the melanistic mutation (gray phenotype) was 0.33, whereas, the frequency was 0.67 for the ancestral allele (red phenotype). Microsatellite diversity of our sampled jaguarundis was less than that of sympatric populations of ocelots (Leopardus pardalis) in Tamaulipas; however, mtDNA diversity was much lower. The frequency of the melanistic mutation was lower in our sample than was previously reported in captive jaguarundis. Our microsatellite loci provided adequate diversity to implement noninvasive genetic tools to better understand the ecology of the elusive jaguarundi. To our knowledge, this work is the first genetic evaluation of wild jaguarundis and provides baseline information for future research and conservation. © 2013 The Wildlife Society.
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spelling doaj-art-533e5190553a404dbd9d581bdf299d6e2025-08-20T02:49:14ZengWileyWildlife Society Bulletin2328-55402013-06-0137233634110.1002/wsb.246Population genetics of jaguarundis in Mexico: Implications for future research and conservationJoseph D. Holbrook0Arturo Caso1Randy W. Deyoung2Michael E. Tewes3Caesar Kleberg Wildlife Research InstituteMSC 218, 700 University Boulevard, Texas A&M University‐KingsvilleKingsvilleTX78363USACaesar Kleberg Wildlife Research InstituteMSC 218, 700 University Boulevard, Texas A&M University‐KingsvilleKingsvilleTX78363USACaesar Kleberg Wildlife Research InstituteMSC 218, 700 University Boulevard, Texas A&M University‐KingsvilleKingsvilleTX78363USACaesar Kleberg Wildlife Research InstituteMSC 218, 700 University Boulevard, Texas A&M University‐KingsvilleKingsvilleTX78363USAAbstract The jaguarundi (Puma yagouaroundi) is a Neotropical felid that ranges from northern Mexico to South America. The population trend for jaguarundis is declining, yet much remains unknown about their ecology. We live‐trapped 11 jaguarundis during 1991–2004 in Tamaulipas, Mexico, and we collected blood for genetic analyses. Our objectives were to 1) estimate neutral diversity using microsatellite and mitochondrial (mtDNA) markers, 2) estimate potentially adaptive diversity in coat coloration via the melanocortin‐1 receptor gene (MC1R), and 3) provide recommendations on the use of genetic markers for noninvasive studies. We observed moderate levels of heterozygosity (HE = 0.49, SD = 0.22) and number of alleles (A = 4.00, SD = 1.65) at 12 feline microsatellite loci. The probability of identifying 2 different jaguarundis as the same individual was low using only 4–7 loci (P(ID) < 0.001 and P(ID)sib < 0.01, respectively). We observed one mtDNA haplotype, indicating no mtDNA diversity. However, we documented diversity with the MC1R assay. The frequency of the melanistic mutation (gray phenotype) was 0.33, whereas, the frequency was 0.67 for the ancestral allele (red phenotype). Microsatellite diversity of our sampled jaguarundis was less than that of sympatric populations of ocelots (Leopardus pardalis) in Tamaulipas; however, mtDNA diversity was much lower. The frequency of the melanistic mutation was lower in our sample than was previously reported in captive jaguarundis. Our microsatellite loci provided adequate diversity to implement noninvasive genetic tools to better understand the ecology of the elusive jaguarundi. To our knowledge, this work is the first genetic evaluation of wild jaguarundis and provides baseline information for future research and conservation. © 2013 The Wildlife Society.https://doi.org/10.1002/wsb.246felidaejaguarundimelanismmelanocortin‐1 receptormicrosatellite locimitochondrial DNA
spellingShingle Joseph D. Holbrook
Arturo Caso
Randy W. Deyoung
Michael E. Tewes
Population genetics of jaguarundis in Mexico: Implications for future research and conservation
Wildlife Society Bulletin
felidae
jaguarundi
melanism
melanocortin‐1 receptor
microsatellite loci
mitochondrial DNA
title Population genetics of jaguarundis in Mexico: Implications for future research and conservation
title_full Population genetics of jaguarundis in Mexico: Implications for future research and conservation
title_fullStr Population genetics of jaguarundis in Mexico: Implications for future research and conservation
title_full_unstemmed Population genetics of jaguarundis in Mexico: Implications for future research and conservation
title_short Population genetics of jaguarundis in Mexico: Implications for future research and conservation
title_sort population genetics of jaguarundis in mexico implications for future research and conservation
topic felidae
jaguarundi
melanism
melanocortin‐1 receptor
microsatellite loci
mitochondrial DNA
url https://doi.org/10.1002/wsb.246
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