Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum
Abstract The plant Polygonum capitatum (P. capitatum) contains a variety of flavonoids that are distributed differently among different parts. Nevertheless, differentially expressed genes (DEGs) associated with this heterogeneous distribution have not been identified. In this study, combined with tr...
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| Format: | Article |
| Language: | English |
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Wiley
2025-03-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.20543 |
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| author | Jie Yang Yu Zhang Bu‐Fa Guo Qi‐Lun Peng Hong‐Yu Chen Mao Ye Wei Yi Wei‐Jun Ding |
| author_facet | Jie Yang Yu Zhang Bu‐Fa Guo Qi‐Lun Peng Hong‐Yu Chen Mao Ye Wei Yi Wei‐Jun Ding |
| author_sort | Jie Yang |
| collection | DOAJ |
| description | Abstract The plant Polygonum capitatum (P. capitatum) contains a variety of flavonoids that are distributed differently among different parts. Nevertheless, differentially expressed genes (DEGs) associated with this heterogeneous distribution have not been identified. In this study, combined with transcriptomic and metabonomic analysis, we identified significant DEGs related to variations in flavonoid composition among different parts of P. capitatum. Subsequently, transcriptomic and nontargeted metabolomic analyses revealed that flavonoids and phenolic acids in different parts of P. capitatum were significantly enriched in the phenylpropanoid biosynthesis, shikimic acid biosynthesis, and flavonoid biosynthesis pathways. The expression levels of genes encoding enzymes, including shikimate O‐hydroxycinnamoyltransferase (HCT), chalcone synthase (CHS), flavonoid 3′,5′‐hydroxylase (CYP75A), flavones 3‐hydroxylase (F3H), flavonol synthase (FLS), leucoanthocyanidin reductase (LAR), trans‐cinnamate 4‐monooxygenase (CYP73A), and shikimate kinase (SK), were found to be the lowest in the leaves of P. capitatum via quantitative PCR. Interestingly, these genes are involved in the biosynthesis of quality markers such as gallic acid, quercetin, and quercitrin in P. capitatum. Finally, the targeted metabolomic results reconfirmed that the gallic acid, quercetin, and quercitrin contents were the highest in the leaves of P. capitatum. This research provides a theoretical basis for further understanding the differential regulatory mechanism of flavonoid metabolism in different parts of P. capitatum, providing novel insights into the pharmacognostic basis of P. capitatum. |
| format | Article |
| id | doaj-art-53349ff8315a4079a95f2d90cea08794 |
| institution | OA Journals |
| issn | 1940-3372 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj-art-53349ff8315a4079a95f2d90cea087942025-08-20T01:50:07ZengWileyThe Plant Genome1940-33722025-03-01181n/an/a10.1002/tpg2.20543Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatumJie Yang0Yu Zhang1Bu‐Fa Guo2Qi‐Lun Peng3Hong‐Yu Chen4Mao Ye5Wei Yi6Wei‐Jun Ding7Department of Fundamental Medicine Bijie Medical College Bijie Guizhou ChinaDepartment of Traditional Chinese Medicine Chongqing Medical and Pharmaceutical College Chongqing ChinaDepartment of Fundamental Medicine Bijie Medical College Bijie Guizhou ChinaDepartment of Fundamental Medicine Bijie Medical College Bijie Guizhou ChinaDepartment of Fundamental Medicine Bijie Medical College Bijie Guizhou ChinaThe Key Laboratory for Health Industry Bijie Medical College Bijie Guizhou ChinaDepartment of Pharmacy Guangzhou Medical University Guangzhou ChinaDepartment of Fundamental Medicine Chengdu University of Traditional Chinese Medicine Chengdu ChinaAbstract The plant Polygonum capitatum (P. capitatum) contains a variety of flavonoids that are distributed differently among different parts. Nevertheless, differentially expressed genes (DEGs) associated with this heterogeneous distribution have not been identified. In this study, combined with transcriptomic and metabonomic analysis, we identified significant DEGs related to variations in flavonoid composition among different parts of P. capitatum. Subsequently, transcriptomic and nontargeted metabolomic analyses revealed that flavonoids and phenolic acids in different parts of P. capitatum were significantly enriched in the phenylpropanoid biosynthesis, shikimic acid biosynthesis, and flavonoid biosynthesis pathways. The expression levels of genes encoding enzymes, including shikimate O‐hydroxycinnamoyltransferase (HCT), chalcone synthase (CHS), flavonoid 3′,5′‐hydroxylase (CYP75A), flavones 3‐hydroxylase (F3H), flavonol synthase (FLS), leucoanthocyanidin reductase (LAR), trans‐cinnamate 4‐monooxygenase (CYP73A), and shikimate kinase (SK), were found to be the lowest in the leaves of P. capitatum via quantitative PCR. Interestingly, these genes are involved in the biosynthesis of quality markers such as gallic acid, quercetin, and quercitrin in P. capitatum. Finally, the targeted metabolomic results reconfirmed that the gallic acid, quercetin, and quercitrin contents were the highest in the leaves of P. capitatum. This research provides a theoretical basis for further understanding the differential regulatory mechanism of flavonoid metabolism in different parts of P. capitatum, providing novel insights into the pharmacognostic basis of P. capitatum.https://doi.org/10.1002/tpg2.20543 |
| spellingShingle | Jie Yang Yu Zhang Bu‐Fa Guo Qi‐Lun Peng Hong‐Yu Chen Mao Ye Wei Yi Wei‐Jun Ding Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum The Plant Genome |
| title | Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum |
| title_full | Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum |
| title_fullStr | Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum |
| title_full_unstemmed | Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum |
| title_short | Combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of Polygonum capitatum |
| title_sort | combined transcriptomic and metabolomic analyses reveal the pharmacognostic mechanism of the metabolism of flavonoids in different parts of polygonum capitatum |
| url | https://doi.org/10.1002/tpg2.20543 |
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