Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish
Intestinal inflammation is a harmful condition in fish that can be triggered by the ingestion of soybean meal. Due to the positive costs-benefits ratio of including soybean meal in farmed fish diets, identifying additives with intestinal anti-inflammatory effects could contribute to solving the issu...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/1639720 |
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| author | Pilar E. Ulloa Camila J. Solís Javiera F. De la Paz Trevor G. S. Alaurent Mario Caruffo Adrián J. Hernández Patricio Dantagnan Carmen G. Feijóo |
| author_facet | Pilar E. Ulloa Camila J. Solís Javiera F. De la Paz Trevor G. S. Alaurent Mario Caruffo Adrián J. Hernández Patricio Dantagnan Carmen G. Feijóo |
| author_sort | Pilar E. Ulloa |
| collection | DOAJ |
| description | Intestinal inflammation is a harmful condition in fish that can be triggered by the ingestion of soybean meal. Due to the positive costs-benefits ratio of including soybean meal in farmed fish diets, identifying additives with intestinal anti-inflammatory effects could contribute to solving the issues caused by this plant protein. This study evaluated the effect of incorporating lactoferrin (LF) into a soybean meal-based diet on intestinal inflammation in zebrafish. Larvae were fed with diets containing 50% soybean meal (50SBM) or 50SBM supplemented with LF to 0.5, 1, 1.5 g/kg (50SBM+LF0.5; 50SBM+LF1.0; 50SBM+LF1.5). The 50SBM+LF1.5 diet was the most efficient and larvae had a reduced number of neutrophils in the intestine compared with 50SBM larvae and an indistinguishable number compared with control larvae. Likewise, the transcription of genes involved in neutrophil migration and intestinal mucosal barrier functions (mmp9, muc2.2, and β-def-1) were increased in 50SBM larvae but were normally expressed in 50SBM+LF1.5 larvae. To determine the influence of intestinal inflammation on the general immune response, larvae were challenged with Edwardsiella tarda. Larvae with intestinal inflammation had increased mortality rate compared to control larvae. Importantly, 50SBM+LF1.5 larvae had a mortality rate lower than control larvae. These results demonstrate that LF displays a dual effect in zebrafish, acting as an intestinal anti-inflammatory agent and improving performance against bacterial infection. |
| format | Article |
| id | doaj-art-53199d1d52d14e2fb707e219b185b1b9 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-53199d1d52d14e2fb707e219b185b1b92025-02-03T01:32:47ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/16397201639720Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in ZebrafishPilar E. Ulloa0Camila J. Solís1Javiera F. De la Paz2Trevor G. S. Alaurent3Mario Caruffo4Adrián J. Hernández5Patricio Dantagnan6Carmen G. Feijóo7Departamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andrés Bello, Republica 217, 8370146 Santiago, ChileDepartamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andrés Bello, Republica 217, 8370146 Santiago, ChileDepartamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andrés Bello, Republica 217, 8370146 Santiago, ChileDepartamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andrés Bello, Republica 217, 8370146 Santiago, ChileDepartamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andrés Bello, Republica 217, 8370146 Santiago, ChileNúcleo de Investigación en Producción Alimentaria, Escuela de Acuicultura, Facultad de Recursos Naturales, Universidad Católica de Temuco, Avenida Rudecindo Ortega 02950, Casilla 15D, 4780000 Temuco, ChileNúcleo de Investigación en Producción Alimentaria, Escuela de Acuicultura, Facultad de Recursos Naturales, Universidad Católica de Temuco, Avenida Rudecindo Ortega 02950, Casilla 15D, 4780000 Temuco, ChileDepartamento de Ciencias Biologicas, Facultad de Ciencias Biologicas, Universidad Andrés Bello, Republica 217, 8370146 Santiago, ChileIntestinal inflammation is a harmful condition in fish that can be triggered by the ingestion of soybean meal. Due to the positive costs-benefits ratio of including soybean meal in farmed fish diets, identifying additives with intestinal anti-inflammatory effects could contribute to solving the issues caused by this plant protein. This study evaluated the effect of incorporating lactoferrin (LF) into a soybean meal-based diet on intestinal inflammation in zebrafish. Larvae were fed with diets containing 50% soybean meal (50SBM) or 50SBM supplemented with LF to 0.5, 1, 1.5 g/kg (50SBM+LF0.5; 50SBM+LF1.0; 50SBM+LF1.5). The 50SBM+LF1.5 diet was the most efficient and larvae had a reduced number of neutrophils in the intestine compared with 50SBM larvae and an indistinguishable number compared with control larvae. Likewise, the transcription of genes involved in neutrophil migration and intestinal mucosal barrier functions (mmp9, muc2.2, and β-def-1) were increased in 50SBM larvae but were normally expressed in 50SBM+LF1.5 larvae. To determine the influence of intestinal inflammation on the general immune response, larvae were challenged with Edwardsiella tarda. Larvae with intestinal inflammation had increased mortality rate compared to control larvae. Importantly, 50SBM+LF1.5 larvae had a mortality rate lower than control larvae. These results demonstrate that LF displays a dual effect in zebrafish, acting as an intestinal anti-inflammatory agent and improving performance against bacterial infection.http://dx.doi.org/10.1155/2016/1639720 |
| spellingShingle | Pilar E. Ulloa Camila J. Solís Javiera F. De la Paz Trevor G. S. Alaurent Mario Caruffo Adrián J. Hernández Patricio Dantagnan Carmen G. Feijóo Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish Journal of Immunology Research |
| title | Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish |
| title_full | Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish |
| title_fullStr | Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish |
| title_full_unstemmed | Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish |
| title_short | Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish |
| title_sort | lactoferrin decreases the intestinal inflammation triggered by a soybean meal based diet in zebrafish |
| url | http://dx.doi.org/10.1155/2016/1639720 |
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