Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic Assessment

Consumers are shifting to a plant-based lifestyle worldwide as more sustainable and healthier alternatives. However, despite the increasing popularity of plant-based vegan products, scientific evidence on their nutritional quality and health effects is still lacking. This study assessed the nutritio...

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Main Authors: Sermin Durak, Ayşe Nur Demirci, Aleyna Çavdar, Yasemin Yılmazer, Serap Andaç, İsmail Hakkı Tekiner
Format: Article
Language:English
Published: Hasan Eleroğlu 2025-04-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:https://agrifoodscience.com/index.php/TURJAF/article/view/7436
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author Sermin Durak
Ayşe Nur Demirci
Aleyna Çavdar
Yasemin Yılmazer
Serap Andaç
İsmail Hakkı Tekiner
author_facet Sermin Durak
Ayşe Nur Demirci
Aleyna Çavdar
Yasemin Yılmazer
Serap Andaç
İsmail Hakkı Tekiner
author_sort Sermin Durak
collection DOAJ
description Consumers are shifting to a plant-based lifestyle worldwide as more sustainable and healthier alternatives. However, despite the increasing popularity of plant-based vegan products, scientific evidence on their nutritional quality and health effects is still lacking. This study assessed the nutritional and cytotoxic characteristics of the vegan and regular cheese varieties sold in the retail markets in Istanbul, Türkiye in terms of total protein content, amino acid profile, vitamin B, calcium, and in vitro cytotoxicity using Kjeldahl, LC-MS/MS, HLPC, ICP-MS, and in vitro MTS assay, respectively. Our findings showed that the protein content in the regular cheese varieties was 20.7%, while it was 13.3% in vegan tofu only. The ratio between essential and non-essential amino acids in the regular and vegan tofu cheeses was 36.0/64.0 and 38.0/62.0, respectively. Vitamins B6, B9, and B12 were detected in none of the varieties, and calcium levels were found to be 568.1 in the regular cheeses and 17.8 mg/100 g in the vegan cheeses. Besides, in vitro, MTS assay demonstrated that regular cheese Roquefort and vegan varieties significantly decreased the cell viability of the HTC-116 human colon cancer cell line. Overall, the current research highlights the need for a comprehensive evaluation of the vegan cheese types better to understand their nutritional, pre- and clinical toxicity, and bioavailability characteristics in a dose and time-dependent manner using advanced techniques and improving the nutritional quality of vegan products remains a challenge for the food industry.
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publisher Hasan Eleroğlu
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spelling doaj-art-531439bce4a249edbf4747db4dfef9ca2025-08-20T02:05:31ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2025-04-011341034104110.24925/turjaf.v13i4.1034-1041.74366137Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic AssessmentSermin Durak0https://orcid.org/0000-0001-6741-4345Ayşe Nur Demirci1https://orcid.org/0000-0002-2665-6927Aleyna Çavdar2https://orcid.org/0000-0002-1835-8911Yasemin Yılmazer3https://orcid.org/0000-0003-2190-073XSerap Andaç4https://orcid.org/0000-0002-6253-4118İsmail Hakkı Tekiner5https://orcid.org/0000-0002-7248-2446Istanbul University-Cerrahpasa, Faculty of Medicine, Department of Medical Microbiology, 34100, Istanbul, TürkiyeIstanbul Sabahattin Zaim University, Faculty of Health Sciences, Department of Nutrition and Dietetics, 34303, Istanbul, TürkiyeIstanbul Gelişim University, Vocational School of Health Services, Department of Biomedical Device Technology, 34310, Istanbul, TürkiyeIstanbul Sabahattin Zaim University, Faculty of Engineering and Natural Sciences, DepartmeIstanbul Sabahattin Zaim University, Faculty of Health Sciences, Department of Nutrition and Dietetics, 34303, Istanbul, TürkiyeIndependent Researcher, Istanbul, TürkiyeConsumers are shifting to a plant-based lifestyle worldwide as more sustainable and healthier alternatives. However, despite the increasing popularity of plant-based vegan products, scientific evidence on their nutritional quality and health effects is still lacking. This study assessed the nutritional and cytotoxic characteristics of the vegan and regular cheese varieties sold in the retail markets in Istanbul, Türkiye in terms of total protein content, amino acid profile, vitamin B, calcium, and in vitro cytotoxicity using Kjeldahl, LC-MS/MS, HLPC, ICP-MS, and in vitro MTS assay, respectively. Our findings showed that the protein content in the regular cheese varieties was 20.7%, while it was 13.3% in vegan tofu only. The ratio between essential and non-essential amino acids in the regular and vegan tofu cheeses was 36.0/64.0 and 38.0/62.0, respectively. Vitamins B6, B9, and B12 were detected in none of the varieties, and calcium levels were found to be 568.1 in the regular cheeses and 17.8 mg/100 g in the vegan cheeses. Besides, in vitro, MTS assay demonstrated that regular cheese Roquefort and vegan varieties significantly decreased the cell viability of the HTC-116 human colon cancer cell line. Overall, the current research highlights the need for a comprehensive evaluation of the vegan cheese types better to understand their nutritional, pre- and clinical toxicity, and bioavailability characteristics in a dose and time-dependent manner using advanced techniques and improving the nutritional quality of vegan products remains a challenge for the food industry.https://agrifoodscience.com/index.php/TURJAF/article/view/7436foodnutritioncytotoxicityvegan cheeseplant-based proteinregular cheese
spellingShingle Sermin Durak
Ayşe Nur Demirci
Aleyna Çavdar
Yasemin Yılmazer
Serap Andaç
İsmail Hakkı Tekiner
Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic Assessment
Turkish Journal of Agriculture: Food Science and Technology
food
nutrition
cytotoxicity
vegan cheese
plant-based protein
regular cheese
title Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic Assessment
title_full Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic Assessment
title_fullStr Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic Assessment
title_full_unstemmed Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic Assessment
title_short Vegan Cheese versus Regular Cheese: A Nutritional and Cytotoxic Assessment
title_sort vegan cheese versus regular cheese a nutritional and cytotoxic assessment
topic food
nutrition
cytotoxicity
vegan cheese
plant-based protein
regular cheese
url https://agrifoodscience.com/index.php/TURJAF/article/view/7436
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AT aysenurdemirci vegancheeseversusregularcheeseanutritionalandcytotoxicassessment
AT aleynacavdar vegancheeseversusregularcheeseanutritionalandcytotoxicassessment
AT yaseminyılmazer vegancheeseversusregularcheeseanutritionalandcytotoxicassessment
AT serapandac vegancheeseversusregularcheeseanutritionalandcytotoxicassessment
AT ismailhakkıtekiner vegancheeseversusregularcheeseanutritionalandcytotoxicassessment