Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources
Proteins play an imperative role in enhancing the nutritional status of the human body. The present study was designed to determine the molecular weight of protein isolates prepared from defatted oilseeds, i.e., sesame, flaxseed, and canola, using SDS-PAGE. The electropherogram revealed protein band...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2022/1926527 |
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| author | Muhammad Sibt-e-Abbas Masood Sadiq Butt Mian N. Riaz Tadesse Fikre Teferra Iahtisham Ul-Haq |
| author_facet | Muhammad Sibt-e-Abbas Masood Sadiq Butt Mian N. Riaz Tadesse Fikre Teferra Iahtisham Ul-Haq |
| author_sort | Muhammad Sibt-e-Abbas |
| collection | DOAJ |
| description | Proteins play an imperative role in enhancing the nutritional status of the human body. The present study was designed to determine the molecular weight of protein isolates prepared from defatted oilseeds, i.e., sesame, flaxseed, and canola, using SDS-PAGE. The electropherogram revealed protein bands ranging from 15 to 65 kDa. Furthermore, proteins were subjected to amino acid profiling followed by calculation of amino acid score with reference to requirement for preschool children. The amino acid profiling results indicated that sesame protein isolates (SPI) exhibited the highest values for aromatic amino acids, histidine, isoleucine, and valine. However, the maximum values for sulfur-containing amino acids were depicted by flaxseed protein isolates (FPI). Moreover, the lysine content was highest in canola protein isolates (CPI). Results indicated better profile and quality of proteins, capable to meet the requirements of essential amino acids, especially for preschoolers. Moreover, the values for the protein digestibility corrected amino acid score (PDCAAS) and in vitro protein digestibility (IVPD) were also determined. Conclusively, protein isolates from defatted oilseeds exhibit better-quality proteins with a balanced amino acid profile. By potential utilization in numerous food products, these proteins can play a pivotal role in fulfilling the nutritional requirements of individuals, especially in developing economies. |
| format | Article |
| id | doaj-art-53112dd43b2d4bb98e81663ad3d7da03 |
| institution | Kabale University |
| issn | 1745-4557 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-53112dd43b2d4bb98e81663ad3d7da032025-08-20T03:36:34ZengWileyJournal of Food Quality1745-45572022-01-01202210.1155/2022/1926527Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional SourcesMuhammad Sibt-e-Abbas0Masood Sadiq Butt1Mian N. Riaz2Tadesse Fikre Teferra3Iahtisham Ul-Haq4Department of Food Science & NutritionNational Institute of Food Science and TechnologyDepartment of Food Science and TechnologyCollege of AgricultureKauser Abdulla Malik School of Life SciencesProteins play an imperative role in enhancing the nutritional status of the human body. The present study was designed to determine the molecular weight of protein isolates prepared from defatted oilseeds, i.e., sesame, flaxseed, and canola, using SDS-PAGE. The electropherogram revealed protein bands ranging from 15 to 65 kDa. Furthermore, proteins were subjected to amino acid profiling followed by calculation of amino acid score with reference to requirement for preschool children. The amino acid profiling results indicated that sesame protein isolates (SPI) exhibited the highest values for aromatic amino acids, histidine, isoleucine, and valine. However, the maximum values for sulfur-containing amino acids were depicted by flaxseed protein isolates (FPI). Moreover, the lysine content was highest in canola protein isolates (CPI). Results indicated better profile and quality of proteins, capable to meet the requirements of essential amino acids, especially for preschoolers. Moreover, the values for the protein digestibility corrected amino acid score (PDCAAS) and in vitro protein digestibility (IVPD) were also determined. Conclusively, protein isolates from defatted oilseeds exhibit better-quality proteins with a balanced amino acid profile. By potential utilization in numerous food products, these proteins can play a pivotal role in fulfilling the nutritional requirements of individuals, especially in developing economies.http://dx.doi.org/10.1155/2022/1926527 |
| spellingShingle | Muhammad Sibt-e-Abbas Masood Sadiq Butt Mian N. Riaz Tadesse Fikre Teferra Iahtisham Ul-Haq Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources Journal of Food Quality |
| title | Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources |
| title_full | Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources |
| title_fullStr | Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources |
| title_full_unstemmed | Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources |
| title_short | Amino Acid Profiling and SDS-PAGE Analysis of Protein Isolates Obtained from Nonconventional Sources |
| title_sort | amino acid profiling and sds page analysis of protein isolates obtained from nonconventional sources |
| url | http://dx.doi.org/10.1155/2022/1926527 |
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