Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines
The anticancer activity of isofuranodiene, extracted from Smyrnium olusatrum, was evaluated in human breast adenocarcinomas MDA-MB 231 and BT 474, and Caucasian prostate adenocarcinoma PC 3 cell lines by MTS assay. MTS assay showed a dose-dependent growth inhibition in the tumor cell lines after iso...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/264829 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849405801509158912 |
|---|---|
| author | Michela Buccioni Diego Dal Ben Catia Lambertucci Filippo Maggi Fabrizio Papa Ajiroghene Thomas Claudia Santinelli Gabriella Marucci |
| author_facet | Michela Buccioni Diego Dal Ben Catia Lambertucci Filippo Maggi Fabrizio Papa Ajiroghene Thomas Claudia Santinelli Gabriella Marucci |
| author_sort | Michela Buccioni |
| collection | DOAJ |
| description | The anticancer activity of isofuranodiene, extracted from Smyrnium olusatrum, was evaluated in human breast adenocarcinomas MDA-MB 231 and BT 474, and Caucasian prostate adenocarcinoma PC 3 cell lines by MTS assay. MTS assay showed a dose-dependent growth inhibition in the tumor cell lines after isofuranodiene treatment. The best antiproliferative activity of the isofuranodiene was found on PC 3 cells with an IC50 value of 29 μM, which was slightly less than the inhibition against the two breast adenocarcinoma cell lines with IC50 values of 59 and 55 μM on MDA-MB 231 and BT 474, respectively. Hoechst 33258 assay was performed in order to study the growth inhibition mechanism in prostate cancer cell line; the results indicate that isofuranodiene induces apoptosis. Overall, the understudy compound has a good anticancer activity especially towards the PC 3. On the contrary, it is less active on Chinese hamster ovary cells (CHO) and human embryonic kidney (HEK 293) appearing as a good candidate as a potential natural anticancer drug with low side effects. |
| format | Article |
| id | doaj-art-530dd58bc49544618cf8d54797d4f78e |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-530dd58bc49544618cf8d54797d4f78e2025-08-20T03:36:34ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/264829264829Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell LinesMichela Buccioni0Diego Dal Ben1Catia Lambertucci2Filippo Maggi3Fabrizio Papa4Ajiroghene Thomas5Claudia Santinelli6Gabriella Marucci7School of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalySchool of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalySchool of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalySchool of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalySchool of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalySchool of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalySchool of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalySchool of Pharmacy, Medicinal Chemistry Unit, University of Camerino, Via S. Agostino 1 62032 Camerino, ItalyThe anticancer activity of isofuranodiene, extracted from Smyrnium olusatrum, was evaluated in human breast adenocarcinomas MDA-MB 231 and BT 474, and Caucasian prostate adenocarcinoma PC 3 cell lines by MTS assay. MTS assay showed a dose-dependent growth inhibition in the tumor cell lines after isofuranodiene treatment. The best antiproliferative activity of the isofuranodiene was found on PC 3 cells with an IC50 value of 29 μM, which was slightly less than the inhibition against the two breast adenocarcinoma cell lines with IC50 values of 59 and 55 μM on MDA-MB 231 and BT 474, respectively. Hoechst 33258 assay was performed in order to study the growth inhibition mechanism in prostate cancer cell line; the results indicate that isofuranodiene induces apoptosis. Overall, the understudy compound has a good anticancer activity especially towards the PC 3. On the contrary, it is less active on Chinese hamster ovary cells (CHO) and human embryonic kidney (HEK 293) appearing as a good candidate as a potential natural anticancer drug with low side effects.http://dx.doi.org/10.1155/2014/264829 |
| spellingShingle | Michela Buccioni Diego Dal Ben Catia Lambertucci Filippo Maggi Fabrizio Papa Ajiroghene Thomas Claudia Santinelli Gabriella Marucci Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines The Scientific World Journal |
| title | Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines |
| title_full | Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines |
| title_fullStr | Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines |
| title_full_unstemmed | Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines |
| title_short | Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines |
| title_sort | antiproliferative evaluation of isofuranodiene on breast and prostate cancer cell lines |
| url | http://dx.doi.org/10.1155/2014/264829 |
| work_keys_str_mv | AT michelabuccioni antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines AT diegodalben antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines AT catialambertucci antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines AT filippomaggi antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines AT fabriziopapa antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines AT ajiroghenethomas antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines AT claudiasantinelli antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines AT gabriellamarucci antiproliferativeevaluationofisofuranodieneonbreastandprostatecancercelllines |