The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles
The properties of the large unilamellar vesicles (LUVs) from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), modified by lipopolysaccharides (LPS) from <i>Salmonella enterica</i> sv. Enteritidis, which mimics Gram-negative bacteria, were studied by various physical methods. LPS,...
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2025-01-01
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| author | Tamás Szabó Zuzana Garaiová Sopio Melikishvili Marek Tatarko Zsófia Keresztes Tibor Hianik |
| author_facet | Tamás Szabó Zuzana Garaiová Sopio Melikishvili Marek Tatarko Zsófia Keresztes Tibor Hianik |
| author_sort | Tamás Szabó |
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| description | The properties of the large unilamellar vesicles (LUVs) from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), modified by lipopolysaccharides (LPS) from <i>Salmonella enterica</i> sv. Enteritidis, which mimics Gram-negative bacteria, were studied by various physical methods. LPS, in the range of 0/20/50 % <i>w</i>/<i>w</i> relative to the lipid, had a regulatory role in the structure of the LUVs toward the lower size, low polydispersity, and over-a-month size stability due to the lower negative zeta potential. The addition of LPS resulted in increased density, which determined the ultrasound velocity and the specific adiabatic compressibility. In a 0.5/1/2 mg/mL concentration range, the total lipid content did not significantly affect the size of LUVs and influenced the density-related attributes similarly to the LPS content. A positive correlation was found between temperature and vesicle size, and a negative correlation was found between temperature and density and compressibility—except for the anomaly behavior at 25 °C, around the melting point of DMPC. |
| format | Article |
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| institution | Kabale University |
| issn | 2313-7673 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Biomimetics |
| spelling | doaj-art-52fdbe09761f4de2b0c83cfe852e66552025-01-24T13:24:45ZengMDPI AGBiomimetics2313-76732025-01-011015510.3390/biomimetics10010055The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid VesiclesTamás Szabó0Zuzana Garaiová1Sopio Melikishvili2Marek Tatarko3Zsófia Keresztes4Tibor Hianik5Functional Interfaces Research Group, Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, 1117 Budapest, HungaryDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska Dolina F1, 84248 Bratislava, SlovakiaInstitute of Medical Physics and Biophysics, Faculty of Medicine, Comenius University, Sasinkova 2, 81372 Bratislava, SlovakiaDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska Dolina F1, 84248 Bratislava, SlovakiaFunctional Interfaces Research Group, Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, Magyar Tudósok Körútja 2, 1117 Budapest, HungaryDepartment of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska Dolina F1, 84248 Bratislava, SlovakiaThe properties of the large unilamellar vesicles (LUVs) from 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), modified by lipopolysaccharides (LPS) from <i>Salmonella enterica</i> sv. Enteritidis, which mimics Gram-negative bacteria, were studied by various physical methods. LPS, in the range of 0/20/50 % <i>w</i>/<i>w</i> relative to the lipid, had a regulatory role in the structure of the LUVs toward the lower size, low polydispersity, and over-a-month size stability due to the lower negative zeta potential. The addition of LPS resulted in increased density, which determined the ultrasound velocity and the specific adiabatic compressibility. In a 0.5/1/2 mg/mL concentration range, the total lipid content did not significantly affect the size of LUVs and influenced the density-related attributes similarly to the LPS content. A positive correlation was found between temperature and vesicle size, and a negative correlation was found between temperature and density and compressibility—except for the anomaly behavior at 25 °C, around the melting point of DMPC.https://www.mdpi.com/2313-7673/10/1/55phospholipid vesicleslipopolysaccharidesaverage sizezeta potentialdensityultrasound velocity |
| spellingShingle | Tamás Szabó Zuzana Garaiová Sopio Melikishvili Marek Tatarko Zsófia Keresztes Tibor Hianik The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles Biomimetics phospholipid vesicles lipopolysaccharides average size zeta potential density ultrasound velocity |
| title | The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles |
| title_full | The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles |
| title_fullStr | The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles |
| title_full_unstemmed | The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles |
| title_short | The Effect of Lipopolysaccharides from <i>Salmonella enterica</i> on the Size, Density, and Compressibility of Phospholipid Vesicles |
| title_sort | effect of lipopolysaccharides from i salmonella enterica i on the size density and compressibility of phospholipid vesicles |
| topic | phospholipid vesicles lipopolysaccharides average size zeta potential density ultrasound velocity |
| url | https://www.mdpi.com/2313-7673/10/1/55 |
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