Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa
Aims. To conduct a genome-wide DNA methylation in individuals with type 2 diabetes, individuals with prediabetes, and control mixed ancestry individuals from South Africa. Methods. We used peripheral blood to perform genome-wide DNA methylation analysis in 3 individuals with screen detected diabetes...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2016/3172093 |
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| author | Tandi E. Matsha Carmen Pheiffer Stephen E. Humphries Junaid Gamieldien Rajiv T. Erasmus Andre P. Kengne |
| author_facet | Tandi E. Matsha Carmen Pheiffer Stephen E. Humphries Junaid Gamieldien Rajiv T. Erasmus Andre P. Kengne |
| author_sort | Tandi E. Matsha |
| collection | DOAJ |
| description | Aims. To conduct a genome-wide DNA methylation in individuals with type 2 diabetes, individuals with prediabetes, and control mixed ancestry individuals from South Africa. Methods. We used peripheral blood to perform genome-wide DNA methylation analysis in 3 individuals with screen detected diabetes, 3 individuals with prediabetes, and 3 individuals with normoglycaemia from the Bellville South Community, Cape Town, South Africa, who were age-, gender-, body mass index-, and duration of residency-matched. Methylated DNA immunoprecipitation (MeDIP) was performed by Arraystar Inc. (Rockville, MD, USA). Results. Hypermethylated DMRs were 1160 (81.97%) and 124 (43.20%), respectively, in individuals with diabetes and prediabetes when both were compared to subjects with normoglycaemia. Our data shows that genes related to the immune system, signal transduction, glucose transport, and pancreas development have altered DNA methylation in subjects with prediabetes and diabetes. Pathway analysis based on the functional analysis mapping of genes to KEGG pathways suggested that the linoleic acid metabolism and arachidonic acid metabolism pathways are hypomethylated in prediabetes and diabetes. Conclusions. Our study suggests that epigenetic changes are likely to be an early process that occurs before the onset of overt diabetes. Detailed analysis of DMRs that shows gradual methylation differences from control versus prediabetes to prediabetes versus diabetes in a larger sample size is required to confirm these findings. |
| format | Article |
| id | doaj-art-52ef05955ad74a80bb4ee554b29f4b5d |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-52ef05955ad74a80bb4ee554b29f4b5d2025-02-03T01:30:49ZengWileyInternational Journal of Endocrinology1687-83371687-83452016-01-01201610.1155/2016/31720933172093Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South AfricaTandi E. Matsha0Carmen Pheiffer1Stephen E. Humphries2Junaid Gamieldien3Rajiv T. Erasmus4Andre P. Kengne5Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Cape Town 7535, South AfricaBiomedical Research and Innovation Platform, South African Medical Research Council, Cape Town 7505, South AfricaCentre for Cardiovascular Genetics, British Heart Foundation Laboratories, Institute of Cardiovascular Science, The Rayne Building, University College London, London WC1E 6JF, UKSouth African National Bioinformatics Institute, University of the Western Cape, Cape Town 7535, South AfricaDivision of Chemical Pathology and National Health Laboratory Service (NHLS), Faculty of Medicine and Health Sciences, University of Stellenbosch, Cape Town 7505, South AfricaNon-Communicable Diseases Research Unit, South African Medical Research Council, Cape Town 7505, South AfricaAims. To conduct a genome-wide DNA methylation in individuals with type 2 diabetes, individuals with prediabetes, and control mixed ancestry individuals from South Africa. Methods. We used peripheral blood to perform genome-wide DNA methylation analysis in 3 individuals with screen detected diabetes, 3 individuals with prediabetes, and 3 individuals with normoglycaemia from the Bellville South Community, Cape Town, South Africa, who were age-, gender-, body mass index-, and duration of residency-matched. Methylated DNA immunoprecipitation (MeDIP) was performed by Arraystar Inc. (Rockville, MD, USA). Results. Hypermethylated DMRs were 1160 (81.97%) and 124 (43.20%), respectively, in individuals with diabetes and prediabetes when both were compared to subjects with normoglycaemia. Our data shows that genes related to the immune system, signal transduction, glucose transport, and pancreas development have altered DNA methylation in subjects with prediabetes and diabetes. Pathway analysis based on the functional analysis mapping of genes to KEGG pathways suggested that the linoleic acid metabolism and arachidonic acid metabolism pathways are hypomethylated in prediabetes and diabetes. Conclusions. Our study suggests that epigenetic changes are likely to be an early process that occurs before the onset of overt diabetes. Detailed analysis of DMRs that shows gradual methylation differences from control versus prediabetes to prediabetes versus diabetes in a larger sample size is required to confirm these findings.http://dx.doi.org/10.1155/2016/3172093 |
| spellingShingle | Tandi E. Matsha Carmen Pheiffer Stephen E. Humphries Junaid Gamieldien Rajiv T. Erasmus Andre P. Kengne Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa International Journal of Endocrinology |
| title | Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa |
| title_full | Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa |
| title_fullStr | Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa |
| title_full_unstemmed | Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa |
| title_short | Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa |
| title_sort | genome wide dna methylation in mixed ancestry individuals with diabetes and prediabetes from south africa |
| url | http://dx.doi.org/10.1155/2016/3172093 |
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