Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis
The genetic diversity of the banana shrimp Fenneropenaeus merguiensis is at risk because of over-harvesting, which consequently reduces food security. This endangerment is exacerbated because this species is not commonly cultivated by farmers. Overall, these factors necessitate conservation of this...
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KeAi Communications Co., Ltd.
2025-01-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X23000862 |
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| author | Jirakanit Chimnual Jirawat Saetan Wilaiwan Chotigeat |
| author_facet | Jirakanit Chimnual Jirawat Saetan Wilaiwan Chotigeat |
| author_sort | Jirakanit Chimnual |
| collection | DOAJ |
| description | The genetic diversity of the banana shrimp Fenneropenaeus merguiensis is at risk because of over-harvesting, which consequently reduces food security. This endangerment is exacerbated because this species is not commonly cultivated by farmers. Overall, these factors necessitate conservation of this shrimp species across its natural habitat. Information on the migration of primordial germ cells (PGCs) to form a gonad is essential for shrimp preservation techniques such as broodstock preparation, sex differentiation, and germ cell transplantation. In this study, histological analysis and in situ hybridization of vasa expression (from embryo to testis development) were used to demonstrate the movement of PGCs. Hematoxylin and Eosin staining and in situ hybridization with the VASA probe revealed that the PGCs migrated retrogradely along the midgut, colonizing the area between the hepatopancreas and heart, a region that becomes the genital ridge in the postlarval stage. External sexual organs appeared at approximately 4 months of age. Through real-time PCR, the expression of the vasa gene was detected early on postlarval day 7, whereas its abundant expression was detected in the ovaries and testes of adult shrimp. This study could help with the identification and monitoring of PGCs or spermatogonia in banana shrimp and facilitate the implementation of other germ cell-relevant techniques in the future. |
| format | Article |
| id | doaj-art-52eddabdfe7b456d8720b0dc297b26d4 |
| institution | Kabale University |
| issn | 2468-550X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Aquaculture and Fisheries |
| spelling | doaj-art-52eddabdfe7b456d8720b0dc297b26d42025-02-09T05:00:51ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2025-01-0110194104Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensisJirakanit Chimnual0Jirawat Saetan1Wilaiwan Chotigeat2Division of Biological Science, Molecular Biotechnology and Bioinformatics Program, Faculty of Science, Prince of Songkla University, Songkhla, 90110, ThailandDivision of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla, 90110, ThailandDivision of Biological Science, Molecular Biotechnology and Bioinformatics Program, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand; Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand; Corresponding author. Division of Biological Science, Molecular Biotechnology and Bioinformatics Program, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand.The genetic diversity of the banana shrimp Fenneropenaeus merguiensis is at risk because of over-harvesting, which consequently reduces food security. This endangerment is exacerbated because this species is not commonly cultivated by farmers. Overall, these factors necessitate conservation of this shrimp species across its natural habitat. Information on the migration of primordial germ cells (PGCs) to form a gonad is essential for shrimp preservation techniques such as broodstock preparation, sex differentiation, and germ cell transplantation. In this study, histological analysis and in situ hybridization of vasa expression (from embryo to testis development) were used to demonstrate the movement of PGCs. Hematoxylin and Eosin staining and in situ hybridization with the VASA probe revealed that the PGCs migrated retrogradely along the midgut, colonizing the area between the hepatopancreas and heart, a region that becomes the genital ridge in the postlarval stage. External sexual organs appeared at approximately 4 months of age. Through real-time PCR, the expression of the vasa gene was detected early on postlarval day 7, whereas its abundant expression was detected in the ovaries and testes of adult shrimp. This study could help with the identification and monitoring of PGCs or spermatogonia in banana shrimp and facilitate the implementation of other germ cell-relevant techniques in the future.http://www.sciencedirect.com/science/article/pii/S2468550X23000862Fenneropenaeus merguiensisPrimordial germ cellSpermatogoniavasa |
| spellingShingle | Jirakanit Chimnual Jirawat Saetan Wilaiwan Chotigeat Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis Aquaculture and Fisheries Fenneropenaeus merguiensis Primordial germ cell Spermatogonia vasa |
| title | Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis |
| title_full | Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis |
| title_fullStr | Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis |
| title_full_unstemmed | Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis |
| title_short | Histological demonstration of gonad development in the banana shrimp, Fenneropenaeus merguiensis |
| title_sort | histological demonstration of gonad development in the banana shrimp fenneropenaeus merguiensis |
| topic | Fenneropenaeus merguiensis Primordial germ cell Spermatogonia vasa |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X23000862 |
| work_keys_str_mv | AT jirakanitchimnual histologicaldemonstrationofgonaddevelopmentinthebananashrimpfenneropenaeusmerguiensis AT jirawatsaetan histologicaldemonstrationofgonaddevelopmentinthebananashrimpfenneropenaeusmerguiensis AT wilaiwanchotigeat histologicaldemonstrationofgonaddevelopmentinthebananashrimpfenneropenaeusmerguiensis |