Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the Striatum
The member of trace-amine associated receptor family, TAAR5 receptor was suggested to recognize tertiary amines, mostly in the olfactory system; however, knocking out the receptor TAAR5 in mice showed an enhancing effect on adult neurogenesis and dopamine neurotransmission in the striatum. To estima...
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2024-11-01
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| author | Anastasia N. Vaganova Zoia S. Fesenko Evgeniya V. Efimova Sergei A. Chekrygin Daria D. Shafranskaya Andrey D. Prjibelski Nataliia V. Katolikova Raul R. Gainetdinov |
| author_facet | Anastasia N. Vaganova Zoia S. Fesenko Evgeniya V. Efimova Sergei A. Chekrygin Daria D. Shafranskaya Andrey D. Prjibelski Nataliia V. Katolikova Raul R. Gainetdinov |
| author_sort | Anastasia N. Vaganova |
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| description | The member of trace-amine associated receptor family, TAAR5 receptor was suggested to recognize tertiary amines, mostly in the olfactory system; however, knocking out the receptor TAAR5 in mice showed an enhancing effect on adult neurogenesis and dopamine neurotransmission in the striatum. To estimate the role of the TAAR5, we performed gene expression profiling of striatal samples from TAAR5 knockout (KO) mice and their wild-type littermates. The higher expression of several genes involved in dopaminergic signaling and the downregulation of genes associated with gliogenesis were revealed in TAAR5-KO mice. Meanwhile, the upregulating effect of TAAR5 knockout on genes was associated with neurogenesis and synaptogenesis. The estimation of cell-type relative abundance through the deconvolution of RNA sequencing data demonstrated that TAAR5-KO striatum samples contain more D2 dopamine receptor-expressing medium spiny neurons but fewer astrocytes than wild-type mice. Our findings indicate that previously identified improvement in cognitive functions and motor coordination in TAAR5-KO mice may activate genes involved in neurogenesis, synaptogenesis, and synapse organization in the striatum. These data suggest that the pharmaceutical targeting of TAAR5 may improve striatum-dependent cognitive or motor functions. At the same time, a more detailed investigation of future TAAR5 antagonists’ effect on glia development is necessary. |
| format | Article |
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| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-52ed6ade49714c86ad072e23f20ca5fd2025-08-20T01:53:53ZengMDPI AGCells2073-44092024-11-011322191010.3390/cells13221910Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the StriatumAnastasia N. Vaganova0Zoia S. Fesenko1Evgeniya V. Efimova2Sergei A. Chekrygin3Daria D. Shafranskaya4Andrey D. Prjibelski5Nataliia V. Katolikova6Raul R. Gainetdinov7Institute of Translational Biomedicine, Saint-Petersburg State University, 199034 Saint-Petersburg, RussiaInstitute of Translational Biomedicine, Saint-Petersburg State University, 199034 Saint-Petersburg, RussiaInstitute of Translational Biomedicine, Saint-Petersburg State University, 199034 Saint-Petersburg, RussiaResource Center “Bio-Bank Center”, Research Park of Saint-Petersburg State University, 198504 Saint-Petersburg, RussiaInstitute of Translational Biomedicine, Saint-Petersburg State University, 199034 Saint-Petersburg, RussiaDepartment of Computer Science, University of Helsinki, 00014 Helsinki, FinlandInstitute of Translational Biomedicine, Saint-Petersburg State University, 199034 Saint-Petersburg, RussiaInstitute of Translational Biomedicine, Saint-Petersburg State University, 199034 Saint-Petersburg, RussiaThe member of trace-amine associated receptor family, TAAR5 receptor was suggested to recognize tertiary amines, mostly in the olfactory system; however, knocking out the receptor TAAR5 in mice showed an enhancing effect on adult neurogenesis and dopamine neurotransmission in the striatum. To estimate the role of the TAAR5, we performed gene expression profiling of striatal samples from TAAR5 knockout (KO) mice and their wild-type littermates. The higher expression of several genes involved in dopaminergic signaling and the downregulation of genes associated with gliogenesis were revealed in TAAR5-KO mice. Meanwhile, the upregulating effect of TAAR5 knockout on genes was associated with neurogenesis and synaptogenesis. The estimation of cell-type relative abundance through the deconvolution of RNA sequencing data demonstrated that TAAR5-KO striatum samples contain more D2 dopamine receptor-expressing medium spiny neurons but fewer astrocytes than wild-type mice. Our findings indicate that previously identified improvement in cognitive functions and motor coordination in TAAR5-KO mice may activate genes involved in neurogenesis, synaptogenesis, and synapse organization in the striatum. These data suggest that the pharmaceutical targeting of TAAR5 may improve striatum-dependent cognitive or motor functions. At the same time, a more detailed investigation of future TAAR5 antagonists’ effect on glia development is necessary.https://www.mdpi.com/2073-4409/13/22/1910trace amine-associated receptorsTAAR5striatumneurogenesisneurogenesisdopamine |
| spellingShingle | Anastasia N. Vaganova Zoia S. Fesenko Evgeniya V. Efimova Sergei A. Chekrygin Daria D. Shafranskaya Andrey D. Prjibelski Nataliia V. Katolikova Raul R. Gainetdinov Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the Striatum Cells trace amine-associated receptors TAAR5 striatum neurogenesis neurogenesis dopamine |
| title | Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the Striatum |
| title_full | Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the Striatum |
| title_fullStr | Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the Striatum |
| title_full_unstemmed | Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the Striatum |
| title_short | Knocking Out TAAR5: A Pathway to Enhanced Neurogenesis and Dopamine Signaling in the Striatum |
| title_sort | knocking out taar5 a pathway to enhanced neurogenesis and dopamine signaling in the striatum |
| topic | trace amine-associated receptors TAAR5 striatum neurogenesis neurogenesis dopamine |
| url | https://www.mdpi.com/2073-4409/13/22/1910 |
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