Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat
ThIPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat (Triticum aestivum L.) production. The present study investigated whether the application of the ThIPK2 gene could increase...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2020-04-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311919627140 |
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| author | Shu-juan ZHANG Yu-lian LI Guo-qi SONG Jie GAO Rong-zhi ZHANG Wei LI Ming-li CHEN Gen-ying LI |
| author_facet | Shu-juan ZHANG Yu-lian LI Guo-qi SONG Jie GAO Rong-zhi ZHANG Wei LI Ming-li CHEN Gen-ying LI |
| author_sort | Shu-juan ZHANG |
| collection | DOAJ |
| description | ThIPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat (Triticum aestivum L.) production. The present study investigated whether the application of the ThIPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized ThIPK2 gene was transferred into common wheat through Agrobacterium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of ThIPK2 in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type (WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content (RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized ThIPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering. |
| format | Article |
| id | doaj-art-52dd680cbd2a4d45bfcfd68b59d5d6d3 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-52dd680cbd2a4d45bfcfd68b59d5d6d32025-08-20T03:58:25ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192020-04-0119494195210.1016/S2095-3119(19)62714-0Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheatShu-juan ZHANG0Yu-lian LI1Guo-qi SONG2Jie GAO3Rong-zhi ZHANG4Wei LI5Ming-li CHEN6Gen-ying LI7Correspondence ZHANG Shu-juan; Crop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaCorrespondence LI Yu-lian; Crop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaCorrespondence LI Gen-ying, Tel: +86-531-66658122; Crop Research Institute, Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement on North Yellow and Huai River Valley, Ministry of Agriculture/National Engineering Laboratory for Wheat and Maize, Jinan 250100, P.R.ChinaThIPK2 is an inositol polyphosphate kinase gene cloned from Thellungiella halophila that participates in diverse cellular processes. Drought is a major limiting factor in wheat (Triticum aestivum L.) production. The present study investigated whether the application of the ThIPK2 gene could increase the drought resistance of transgenic wheat. The codon-optimized ThIPK2 gene was transferred into common wheat through Agrobacterium-mediated transformation driven by either a constitutive maize ubiquitin promoter or a stress-inducible rd29A promoter from Arabidopsis. Molecular characterization confirmed the presence of the foreign gene in the transformed plants. The transgenic expression of ThIPK2 in wheat led to significantly improve drought tolerance compared to that observed in control plants. Compared to the wild type (WT) plants, the transgenic plants showed higher seed germination rates, better developed root systems, a higher relative water content (RWC) and total soluble sugar content, and less cell membrane damage under drought stress conditions. The expression profiles showed different expression patterns with the use of different promoters. The codon-optimized ThIPK2 gene is a candidate gene to enhance wheat drought stress tolerance by genetic engineering.http://www.sciencedirect.com/science/article/pii/S2095311919627140codon-optimizeddroughtrd29AThIPK2Triticum aestivum L. |
| spellingShingle | Shu-juan ZHANG Yu-lian LI Guo-qi SONG Jie GAO Rong-zhi ZHANG Wei LI Ming-li CHEN Gen-ying LI Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat Journal of Integrative Agriculture codon-optimized drought rd29A ThIPK2 Triticum aestivum L. |
| title | Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat |
| title_full | Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat |
| title_fullStr | Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat |
| title_full_unstemmed | Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat |
| title_short | Heterologous expression of the ThIPK2 gene enhances drought resistance of common wheat |
| title_sort | heterologous expression of the thipk2 gene enhances drought resistance of common wheat |
| topic | codon-optimized drought rd29A ThIPK2 Triticum aestivum L. |
| url | http://www.sciencedirect.com/science/article/pii/S2095311919627140 |
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