Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamine

Cannabis remains by far the most consumed illicit drug in Europe. The availability of more potent cannabis has raised concerns regarding the enhanced health risks associated with its use, particularly among pregnant women. Growing evidence shows that cannabis use during pregnancy increases the risks...

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Main Authors: Serra Valeria, Traccis Francesco, Aroni Sonia, Vidal Palencia Laura, Concas Luca, Serra Marcello, Leone Roberta, Porcu Patrizia, Busquets Garcia Arnau, Frau Roberto, Melis Miriam
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Pharmacological Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S104366182400481X
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author Serra Valeria
Traccis Francesco
Aroni Sonia
Vidal Palencia Laura
Concas Luca
Serra Marcello
Leone Roberta
Porcu Patrizia
Busquets Garcia Arnau
Frau Roberto
Melis Miriam
author_facet Serra Valeria
Traccis Francesco
Aroni Sonia
Vidal Palencia Laura
Concas Luca
Serra Marcello
Leone Roberta
Porcu Patrizia
Busquets Garcia Arnau
Frau Roberto
Melis Miriam
author_sort Serra Valeria
collection DOAJ
description Cannabis remains by far the most consumed illicit drug in Europe. The availability of more potent cannabis has raised concerns regarding the enhanced health risks associated with its use, particularly among pregnant women. Growing evidence shows that cannabis use during pregnancy increases the risks of child psychopathology. We have previously shown that only male rat offspring prenatally exposed to Δ9-tetrahydrocannabinol (THC), a rat model of prenatal cannabinoid exposure (PCE), display a hyperdopaminergic phenotype associated with a differential susceptibility to acute THC- and stress-mediated effects on sensorimotor gating functions. Here, we explore the contribution of the hypothalamic-pituitary-adrenal (HPA) axis, key regulator of body adaptive stress responses, to the detrimental effects of acute stress on ventral tegmental area (VTA) dopamine neurons and sensorimotor gating function of PCE rats. We report a sex-dependent compromised balance in mRNA levels of genes encoding mineralocorticoid and glucocorticoid receptors in the VTA, alongside with stress-induced pre-pulse inhibition (PPI) impairment. Notably, VTA dopamine neuronal activity is causally linked to the manifestation of stress-dependent deterioration of PPI. Finally, pharmacological manipulations targeting glycogen-synthase-kinase-3-β signaling during postnatal development correct these stress-induced, sex-specific and dopamine-dependent disruption of PPI. Collectively, these results highlight the critical sex-dependent interplay between HPA axis and dopamine system in the regulation of sensorimotor gating functions in rats.
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spelling doaj-art-52d7e82ac1b94fefbad3ff89911f53ab2025-08-20T02:36:59ZengElsevierPharmacological Research1096-11862024-12-0121010753610.1016/j.phrs.2024.107536Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamineSerra Valeria0Traccis Francesco1Aroni Sonia2Vidal Palencia Laura3Concas Luca4Serra Marcello5Leone Roberta6Porcu Patrizia7Busquets Garcia Arnau8Frau Roberto9Melis Miriam10Dept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, ItalyDept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, ItalyDept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, ItalyHospital del Mar Research Institute, Barcelona, SpainDept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, ItalyDept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, ItalyDept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, ItalyInstitute of Neurosciences, National Research Council (C.N.R.), Cagliari, ItalyHospital del Mar Research Institute, Barcelona, SpainDept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, ItalyDept. Biomedical Sciences, Div. Neuroscience and Clinical Pharmacology, University of Cagliari, Italy; Correspondence to: Department of Biomedical Sciences, Division of Neuroscience and Clinical Pharmacology, Cittadella Universitaria di Monserrato, Monserrato, CA 09042, ItalyCannabis remains by far the most consumed illicit drug in Europe. The availability of more potent cannabis has raised concerns regarding the enhanced health risks associated with its use, particularly among pregnant women. Growing evidence shows that cannabis use during pregnancy increases the risks of child psychopathology. We have previously shown that only male rat offspring prenatally exposed to Δ9-tetrahydrocannabinol (THC), a rat model of prenatal cannabinoid exposure (PCE), display a hyperdopaminergic phenotype associated with a differential susceptibility to acute THC- and stress-mediated effects on sensorimotor gating functions. Here, we explore the contribution of the hypothalamic-pituitary-adrenal (HPA) axis, key regulator of body adaptive stress responses, to the detrimental effects of acute stress on ventral tegmental area (VTA) dopamine neurons and sensorimotor gating function of PCE rats. We report a sex-dependent compromised balance in mRNA levels of genes encoding mineralocorticoid and glucocorticoid receptors in the VTA, alongside with stress-induced pre-pulse inhibition (PPI) impairment. Notably, VTA dopamine neuronal activity is causally linked to the manifestation of stress-dependent deterioration of PPI. Finally, pharmacological manipulations targeting glycogen-synthase-kinase-3-β signaling during postnatal development correct these stress-induced, sex-specific and dopamine-dependent disruption of PPI. Collectively, these results highlight the critical sex-dependent interplay between HPA axis and dopamine system in the regulation of sensorimotor gating functions in rats.http://www.sciencedirect.com/science/article/pii/S104366182400481XCannabisDopamineDREADDHPA axisNeurodevelopmentPsychopathology
spellingShingle Serra Valeria
Traccis Francesco
Aroni Sonia
Vidal Palencia Laura
Concas Luca
Serra Marcello
Leone Roberta
Porcu Patrizia
Busquets Garcia Arnau
Frau Roberto
Melis Miriam
Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamine
Pharmacological Research
Cannabis
Dopamine
DREADD
HPA axis
Neurodevelopment
Psychopathology
title Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamine
title_full Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamine
title_fullStr Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamine
title_full_unstemmed Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamine
title_short Sex-specific maladaptive responses to acute stress upon in utero THC exposure are mediated by dopamine
title_sort sex specific maladaptive responses to acute stress upon in utero thc exposure are mediated by dopamine
topic Cannabis
Dopamine
DREADD
HPA axis
Neurodevelopment
Psychopathology
url http://www.sciencedirect.com/science/article/pii/S104366182400481X
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