miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus
MicroRNA (miRNA) has been widely suggested to play a vital role of in the pathogenesis of gestational diabetes mellitus (GDM). We have previously demonstrated that miR-657 can regulate macrophage inflammatory response in GDM. However, the role of miR-657 on M1/M2 macrophage polarization in GDM patho...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/4851214 |
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| author | Pingping Wang Zengfang Wang Guojie Liu Chengwen Jin Quan Zhang Shuhong Man Zengyan Wang |
| author_facet | Pingping Wang Zengfang Wang Guojie Liu Chengwen Jin Quan Zhang Shuhong Man Zengyan Wang |
| author_sort | Pingping Wang |
| collection | DOAJ |
| description | MicroRNA (miRNA) has been widely suggested to play a vital role of in the pathogenesis of gestational diabetes mellitus (GDM). We have previously demonstrated that miR-657 can regulate macrophage inflammatory response in GDM. However, the role of miR-657 on M1/M2 macrophage polarization in GDM pathogenesis is not clear yet. This study is aimed at elucidating this issue and identifying novel potential GDM therapeutic targets based on miRNA network. miR-657 is found to be upregulated in placental macrophages demonstrated by real-time PCR, which can enhance macrophage proliferation and migration in vitro. Luciferase reporter assay shows the evidence that FAM46C is a target of miR-657. In addition, miR-657 can promote macrophage polarization toward the M1 phenotype by downregulating FAM46C in macrophages. The present study strongly suggests miR-657 is involved in GDM pathogenesis by regulating macrophage proliferation, migration, and polarization via targeting FAM46C. miR-657/FAM46C may serve as promising targets for GDM diagnosis and treatment. |
| format | Article |
| id | doaj-art-52d4cc4c31fb453aa3635014ea5c2eff |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-52d4cc4c31fb453aa3635014ea5c2eff2025-08-20T03:36:34ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/48512144851214miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes MellitusPingping Wang0Zengfang Wang1Guojie Liu2Chengwen Jin3Quan Zhang4Shuhong Man5Zengyan Wang6Department of Gynecology and Obstetrics, Weifang Hospital of Maternal and Child Health, Weifang 261000, ChinaDepartment of Gynecology and Obstetrics, Weifang Hospital of Maternal and Child Health, Weifang 261000, ChinaDepartment of Gynecology and Obstetrics, Weifang Hospital of Maternal and Child Health, Weifang 261000, ChinaFunctional Laboratory, Clinical Medicine College of Weifang Medical University, Weifang 261000, ChinaDepartment of Cardiology, Clinical Medicine College, Weifang Medical University, Weifang 261000, ChinaDepartment of Gynecology, People’s Hospital of Weifang, Weifang 261000, ChinaOperating Room, Zhucheng People’s Hospital, Zhucheng 262200, ChinaMicroRNA (miRNA) has been widely suggested to play a vital role of in the pathogenesis of gestational diabetes mellitus (GDM). We have previously demonstrated that miR-657 can regulate macrophage inflammatory response in GDM. However, the role of miR-657 on M1/M2 macrophage polarization in GDM pathogenesis is not clear yet. This study is aimed at elucidating this issue and identifying novel potential GDM therapeutic targets based on miRNA network. miR-657 is found to be upregulated in placental macrophages demonstrated by real-time PCR, which can enhance macrophage proliferation and migration in vitro. Luciferase reporter assay shows the evidence that FAM46C is a target of miR-657. In addition, miR-657 can promote macrophage polarization toward the M1 phenotype by downregulating FAM46C in macrophages. The present study strongly suggests miR-657 is involved in GDM pathogenesis by regulating macrophage proliferation, migration, and polarization via targeting FAM46C. miR-657/FAM46C may serve as promising targets for GDM diagnosis and treatment.http://dx.doi.org/10.1155/2019/4851214 |
| spellingShingle | Pingping Wang Zengfang Wang Guojie Liu Chengwen Jin Quan Zhang Shuhong Man Zengyan Wang miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus Mediators of Inflammation |
| title | miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus |
| title_full | miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus |
| title_fullStr | miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus |
| title_full_unstemmed | miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus |
| title_short | miR-657 Promotes Macrophage Polarization toward M1 by Targeting FAM46C in Gestational Diabetes Mellitus |
| title_sort | mir 657 promotes macrophage polarization toward m1 by targeting fam46c in gestational diabetes mellitus |
| url | http://dx.doi.org/10.1155/2019/4851214 |
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