Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients.
<h4>Background</h4>Grass pollen, in particular from Lolium multiflorum is a major allergen source in temperate climate zones of Southern Brazil. The IgE sensitization profile of Brazilian grass pollen allergic patients to individual allergen molecules has not been analyzed yet.<h4>...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0128402 |
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| author | Priscila Ferreira de Sousa Moreira Katharina Gangl Francisco de Assis Machado Vieira Leandro Hideki Ynoue Birgit Linhart Sabine Flicker Helmut Fiebig Ines Swoboda Margarete Focke-Tejkl Ernesto Akio Taketomi Rudolf Valenta Verena Niederberger |
| author_facet | Priscila Ferreira de Sousa Moreira Katharina Gangl Francisco de Assis Machado Vieira Leandro Hideki Ynoue Birgit Linhart Sabine Flicker Helmut Fiebig Ines Swoboda Margarete Focke-Tejkl Ernesto Akio Taketomi Rudolf Valenta Verena Niederberger |
| author_sort | Priscila Ferreira de Sousa Moreira |
| collection | DOAJ |
| description | <h4>Background</h4>Grass pollen, in particular from Lolium multiflorum is a major allergen source in temperate climate zones of Southern Brazil. The IgE sensitization profile of Brazilian grass pollen allergic patients to individual allergen molecules has not been analyzed yet.<h4>Objective</h4>To analyze the IgE sensitization profile of a Brazilian grass pollen allergic population using individual allergen molecules.<h4>Methods</h4>We analyzed sera from 78 grass pollen allergic patients for the presence of IgE antibodies specific for 103 purified micro-arrayed natural and recombinant allergens by chip technology. IgE-ELISA inhibition experiments with Lolium multiflorum, Phleum pratense extracts and a recombinant fusion protein consisting of Phl p 1, Phl p 2, Phl p 5 and Phl p 6 were performed to investigate cross-reactivities.<h4>Results</h4>Within the Brazilian grass pollen allergic patients, the most frequently recognized allergens were Phl p 1 (95%), Phl p 5 (82%), Phl p 2 (76%) followed by Phl p 4 (64%), Phl p 6 (45%), Phl p 11 (18%) and Phl p 12 (18%). Most patients were sensitized only to grass pollen allergens but not to allergens from other sources. A high degree of IgE cross-reactivity between Phleum pratense, Lolium multiflorum and the recombinant timothy grass fusion protein was found.<h4>Conclusions</h4>Component-resolved analysis of sera from Brazilian grass pollen allergic patients reveals an IgE recognition profile compatible with a typical Pooideae sensitization. The high degree of cross-reactivity between Phleum pratense and Lolium multiflorum allergens suggests that diagnosis and immunotherapy can be achieved with timothy grass pollen allergens in the studied population. |
| format | Article |
| id | doaj-art-52cbfb2631ec455ba0a0fa7273a5faf6 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-52cbfb2631ec455ba0a0fa7273a5faf62025-08-20T03:10:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012840210.1371/journal.pone.0128402Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients.Priscila Ferreira de Sousa MoreiraKatharina GanglFrancisco de Assis Machado VieiraLeandro Hideki YnoueBirgit LinhartSabine FlickerHelmut FiebigInes SwobodaMargarete Focke-TejklErnesto Akio TaketomiRudolf ValentaVerena Niederberger<h4>Background</h4>Grass pollen, in particular from Lolium multiflorum is a major allergen source in temperate climate zones of Southern Brazil. The IgE sensitization profile of Brazilian grass pollen allergic patients to individual allergen molecules has not been analyzed yet.<h4>Objective</h4>To analyze the IgE sensitization profile of a Brazilian grass pollen allergic population using individual allergen molecules.<h4>Methods</h4>We analyzed sera from 78 grass pollen allergic patients for the presence of IgE antibodies specific for 103 purified micro-arrayed natural and recombinant allergens by chip technology. IgE-ELISA inhibition experiments with Lolium multiflorum, Phleum pratense extracts and a recombinant fusion protein consisting of Phl p 1, Phl p 2, Phl p 5 and Phl p 6 were performed to investigate cross-reactivities.<h4>Results</h4>Within the Brazilian grass pollen allergic patients, the most frequently recognized allergens were Phl p 1 (95%), Phl p 5 (82%), Phl p 2 (76%) followed by Phl p 4 (64%), Phl p 6 (45%), Phl p 11 (18%) and Phl p 12 (18%). Most patients were sensitized only to grass pollen allergens but not to allergens from other sources. A high degree of IgE cross-reactivity between Phleum pratense, Lolium multiflorum and the recombinant timothy grass fusion protein was found.<h4>Conclusions</h4>Component-resolved analysis of sera from Brazilian grass pollen allergic patients reveals an IgE recognition profile compatible with a typical Pooideae sensitization. The high degree of cross-reactivity between Phleum pratense and Lolium multiflorum allergens suggests that diagnosis and immunotherapy can be achieved with timothy grass pollen allergens in the studied population.https://doi.org/10.1371/journal.pone.0128402 |
| spellingShingle | Priscila Ferreira de Sousa Moreira Katharina Gangl Francisco de Assis Machado Vieira Leandro Hideki Ynoue Birgit Linhart Sabine Flicker Helmut Fiebig Ines Swoboda Margarete Focke-Tejkl Ernesto Akio Taketomi Rudolf Valenta Verena Niederberger Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients. PLoS ONE |
| title | Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients. |
| title_full | Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients. |
| title_fullStr | Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients. |
| title_full_unstemmed | Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients. |
| title_short | Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients. |
| title_sort | allergen microarray indicates pooideae sensitization in brazilian grass pollen allergic patients |
| url | https://doi.org/10.1371/journal.pone.0128402 |
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