Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization
Herein, a green and facile enzyme immobilization method was developed by immobilizing enzymes on the bamboo charcoal (BC) through gold-mediated adsorption under mild conditions (no organic solvents were required). Lipase PS was used as the model enzyme, and the obtained immobilized lipase (PS@BC-Au)...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2026-03-01
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| Series: | Journal of Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S277256692500076X |
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| author | Guihong Yao Yun Ling Tong Liu Shige Xing Meiyi Yao Wei Guo Feng Zhang |
| author_facet | Guihong Yao Yun Ling Tong Liu Shige Xing Meiyi Yao Wei Guo Feng Zhang |
| author_sort | Guihong Yao |
| collection | DOAJ |
| description | Herein, a green and facile enzyme immobilization method was developed by immobilizing enzymes on the bamboo charcoal (BC) through gold-mediated adsorption under mild conditions (no organic solvents were required). Lipase PS was used as the model enzyme, and the obtained immobilized lipase (PS@BC-Au) exhibited a high immobilization efficiency (93.9%) and enzyme loading capacity (234.78 µg/mg). The half-life of PS@BC-Au was found to be 362.8 min, which is approximately 1.3 and 2 times longer than that of the free enzyme (185.3 min) and the immobilized enzyme without gold (PS@BC, 275.0 min). Furthermore, PS@BC-Au and PS@BC exhibited up to 63.9% and 47.1% of residual activity after 10 cycles, respectively. Interestingly, the gold nanoparticles in the immobilized system can improve the catalytic efficiency. Consequently, BC, with its porous structure, good stability, large surface area, low cost, and environmental-friendly properties, can serve as a promising support for enzyme immobilized application. |
| format | Article |
| id | doaj-art-52c586c2303e44bf86b4d0a8ff9f8eff |
| institution | Kabale University |
| issn | 2772-5669 |
| language | English |
| publishDate | 2026-03-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Journal of Future Foods |
| spelling | doaj-art-52c586c2303e44bf86b4d0a8ff9f8eff2025-08-20T03:59:22ZengKeAi Communications Co. Ltd.Journal of Future Foods2772-56692026-03-016224425010.1016/j.jfutfo.2024.09.005Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilizationGuihong Yao0Yun Ling1Tong Liu2Shige Xing3Meiyi Yao4Wei Guo5Feng Zhang6Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, ChinaInstitute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, ChinaInstitute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, ChinaInstitute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, ChinaInstitute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, ChinaInstitute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, ChinaInstitute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China; Key Laboratory of Food Quality and Safety, State Administration for Market Regulation, Beijing 100176, China; Corresponding author at: Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.Herein, a green and facile enzyme immobilization method was developed by immobilizing enzymes on the bamboo charcoal (BC) through gold-mediated adsorption under mild conditions (no organic solvents were required). Lipase PS was used as the model enzyme, and the obtained immobilized lipase (PS@BC-Au) exhibited a high immobilization efficiency (93.9%) and enzyme loading capacity (234.78 µg/mg). The half-life of PS@BC-Au was found to be 362.8 min, which is approximately 1.3 and 2 times longer than that of the free enzyme (185.3 min) and the immobilized enzyme without gold (PS@BC, 275.0 min). Furthermore, PS@BC-Au and PS@BC exhibited up to 63.9% and 47.1% of residual activity after 10 cycles, respectively. Interestingly, the gold nanoparticles in the immobilized system can improve the catalytic efficiency. Consequently, BC, with its porous structure, good stability, large surface area, low cost, and environmental-friendly properties, can serve as a promising support for enzyme immobilized application.http://www.sciencedirect.com/science/article/pii/S277256692500076XBamboo charcoalGold nanoparticlesEnzyme immobilization |
| spellingShingle | Guihong Yao Yun Ling Tong Liu Shige Xing Meiyi Yao Wei Guo Feng Zhang Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization Journal of Future Foods Bamboo charcoal Gold nanoparticles Enzyme immobilization |
| title | Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization |
| title_full | Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization |
| title_fullStr | Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization |
| title_full_unstemmed | Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization |
| title_short | Mesoporous bamboo charcoal-gold nanoparticles as a support for efficient enzyme immobilization |
| title_sort | mesoporous bamboo charcoal gold nanoparticles as a support for efficient enzyme immobilization |
| topic | Bamboo charcoal Gold nanoparticles Enzyme immobilization |
| url | http://www.sciencedirect.com/science/article/pii/S277256692500076X |
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