Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods
Uncultured microorganisms represent a promising and untapped source of antibacterial compounds, crucial in the fight against the significant threat of antimicrobial resistance (AMR). In this study, both traditional and isolation chip (iChip) cultivation techniques were employed to enhance the recove...
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MDPI AG
2024-11-01
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| Online Access: | https://www.mdpi.com/2076-2607/12/12/2422 |
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| author | Fabien Perrier Juliette Morice Sabrina Gueulle Antoine Géry Eliette Riboulet-Bisson David Garon Cécile Muller Florie Desriac |
| author_facet | Fabien Perrier Juliette Morice Sabrina Gueulle Antoine Géry Eliette Riboulet-Bisson David Garon Cécile Muller Florie Desriac |
| author_sort | Fabien Perrier |
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| description | Uncultured microorganisms represent a promising and untapped source of antibacterial compounds, crucial in the fight against the significant threat of antimicrobial resistance (AMR). In this study, both traditional and isolation chip (iChip) cultivation techniques were employed to enhance the recovery of known and unknown microorganisms from soils located in Normandy, France. The isolates obtained were identified using 16S rDNA or ITS regions analysis and MALDI-TOF mass spectrometry and were screened for antibacterial activity. A total of 386 isolates, belonging to 6 microbial phyla and distributed across 65 genera, were recovered using both methods. In total, 11 isolates are potentially new bacterial species, and 34 were associated with 22 species described recently. The iChip method yielded a higher diversity of microorganisms (47 genera) than the traditional method (38 genera) and was particularly effective in enriching <i>Actinomycetota</i>. Antibacterial screening against target bacteria showed that 85 isolates (22%) exhibited antibacterial activity. The <i>Streptomyces</i>, <i>Pseudomonas</i>, and <i>Bacillaceae</i> taxa accounted for most antibacterial-producing bacteria with some presenting promising undescribed characteristics. Other active isolates were affiliated with less-known antibacterial producers such as <i>Arthrobacter</i>, <i>Chryseobacterium</i>, <i>Delftia</i>, <i>Ensifer</i>, <i>Flavobacterium</i>, <i>Rahnella</i>, and <i>Stenotrophomonas</i>, among others. These results highlight the potential of our microbial collection as a source of new antibacterial natural products. |
| format | Article |
| id | doaj-art-52c514814de647fa8360fc7e5bdd82e9 |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Microorganisms |
| spelling | doaj-art-52c514814de647fa8360fc7e5bdd82e92025-08-20T02:01:10ZengMDPI AGMicroorganisms2076-26072024-11-011212242210.3390/microorganisms12122422Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip MethodsFabien Perrier0Juliette Morice1Sabrina Gueulle2Antoine Géry3Eliette Riboulet-Bisson4David Garon5Cécile Muller6Florie Desriac7Université de Caen Normandie, CBSA UR 4312, UFR des Sciences, Campus 1, F-14000 Caen, FranceUniversité de Caen Normandie, CBSA UR 4312, UFR des Sciences, Campus 1, F-14000 Caen, FranceUniversité de Caen Normandie, CBSA UR 4312, UFR des Sciences, Campus 1, F-14000 Caen, FranceUniversité de Caen Normandie, ToxEMAC-ABTE UR 4651, UFR des Sciences, Campus 1, F-14000 Caen, FranceUniversité de Caen Normandie, CBSA UR 4312, UFR des Sciences, Campus 1, F-14000 Caen, FranceUniversité de Caen Normandie, ToxEMAC-ABTE UR 4651, UFR des Sciences, Campus 1, F-14000 Caen, FranceUniversité de Caen Normandie, CBSA UR 4312, UFR des Sciences, Campus 1, F-14000 Caen, FranceUniversité de Caen Normandie, CBSA UR 4312, UFR des Sciences, Campus 1, F-14000 Caen, FranceUncultured microorganisms represent a promising and untapped source of antibacterial compounds, crucial in the fight against the significant threat of antimicrobial resistance (AMR). In this study, both traditional and isolation chip (iChip) cultivation techniques were employed to enhance the recovery of known and unknown microorganisms from soils located in Normandy, France. The isolates obtained were identified using 16S rDNA or ITS regions analysis and MALDI-TOF mass spectrometry and were screened for antibacterial activity. A total of 386 isolates, belonging to 6 microbial phyla and distributed across 65 genera, were recovered using both methods. In total, 11 isolates are potentially new bacterial species, and 34 were associated with 22 species described recently. The iChip method yielded a higher diversity of microorganisms (47 genera) than the traditional method (38 genera) and was particularly effective in enriching <i>Actinomycetota</i>. Antibacterial screening against target bacteria showed that 85 isolates (22%) exhibited antibacterial activity. The <i>Streptomyces</i>, <i>Pseudomonas</i>, and <i>Bacillaceae</i> taxa accounted for most antibacterial-producing bacteria with some presenting promising undescribed characteristics. Other active isolates were affiliated with less-known antibacterial producers such as <i>Arthrobacter</i>, <i>Chryseobacterium</i>, <i>Delftia</i>, <i>Ensifer</i>, <i>Flavobacterium</i>, <i>Rahnella</i>, and <i>Stenotrophomonas</i>, among others. These results highlight the potential of our microbial collection as a source of new antibacterial natural products.https://www.mdpi.com/2076-2607/12/12/2422antimicrobial resistanceiChipin situ cultivationuncultured bacteriafungiantibacterial activity |
| spellingShingle | Fabien Perrier Juliette Morice Sabrina Gueulle Antoine Géry Eliette Riboulet-Bisson David Garon Cécile Muller Florie Desriac Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods Microorganisms antimicrobial resistance iChip in situ cultivation uncultured bacteria fungi antibacterial activity |
| title | Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods |
| title_full | Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods |
| title_fullStr | Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods |
| title_full_unstemmed | Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods |
| title_short | Assessing Normandy Soil Microbial Diversity for Antibacterial Activities Using Traditional Culture and iChip Methods |
| title_sort | assessing normandy soil microbial diversity for antibacterial activities using traditional culture and ichip methods |
| topic | antimicrobial resistance iChip in situ cultivation uncultured bacteria fungi antibacterial activity |
| url | https://www.mdpi.com/2076-2607/12/12/2422 |
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