A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae

A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae

Klebsiella pneumoniae (K. pneumoniae) was a prevalent nosocomial pathogen and a major cause of neonatal sepsis, highlighting the critical need for simple, rapid, sensitive and reliable diagnosis for K. pneumoniae infection. While CRISPR-based diagnostics provide a transformative technology to nuclei...

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Bibliographic Details
Main Authors: Juan Zhou, Zirong Wu, Lei Yu, Xiaolan Huang, Fei Xiao, Yu Zhang, Linglong Wan, Jin Fu, Nan Jia, Min Chen, Chunrong Sun, Zhaomin Feng, Yi Wang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Sensing and Bio-Sensing Research
Subjects:
K. pneumoniae
Multiple cross displacement amplification
CRISPR-KP
AapCas12b
One-step
Online Access:http://www.sciencedirect.com/science/article/pii/S2214180425000637
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