DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGS
The developed method for the quantitative determination of phenibut in tablet drugs is based on the dansylation reaction in an alkaline medium (pH 9.18). The reaction results in the formation of a fluorescent derivative with λem=505 nm and λex=335 nm. Optimal conditions were selected to complete the...
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| Format: | Article |
| Language: | Russian |
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LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2019-01-01
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| Series: | Разработка и регистрация лекарственных средств |
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| Online Access: | https://www.pharmjournal.ru/jour/article/view/504 |
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| _version_ | 1849243892337082368 |
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| author | O. I. Lazovskaya V. N. Leontiev E. V. Litvinova |
| author_facet | O. I. Lazovskaya V. N. Leontiev E. V. Litvinova |
| author_sort | O. I. Lazovskaya |
| collection | DOAJ |
| description | The developed method for the quantitative determination of phenibut in tablet drugs is based on the dansylation reaction in an alkaline medium (pH 9.18). The reaction results in the formation of a fluorescent derivative with λem=505 nm and λex=335 nm. Optimal conditions were selected to complete the reaction: temperature and time of thermostatting (40 °C and 15 min), molar ratio of phenibut and dansyl chloride (1:1.4). The method was validated in parameters of specificity, linearity, precision and accuracy. A plot of fluorescence intensity versus concentration of phenibut over the range of 800-1200 ng/ml is described by the regression equation y =0.1003x+1.4685 ( r =0.9999). The limit of detection and limit of quantification were 29.55 и 89.54 ng/ml respectively. |
| format | Article |
| id | doaj-art-52c0d70462a64c8288f6e273c87f9b52 |
| institution | Kabale University |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| publishDate | 2019-01-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj-art-52c0d70462a64c8288f6e273c87f9b522025-08-20T03:59:20ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492019-01-0104104107504DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGSO. I. Lazovskaya0V. N. Leontiev1E. V. Litvinova2Belarusian State Technological UniversityBelarusian State Technological UniversityRUE «Belmedpreparaty»The developed method for the quantitative determination of phenibut in tablet drugs is based on the dansylation reaction in an alkaline medium (pH 9.18). The reaction results in the formation of a fluorescent derivative with λem=505 nm and λex=335 nm. Optimal conditions were selected to complete the reaction: temperature and time of thermostatting (40 °C and 15 min), molar ratio of phenibut and dansyl chloride (1:1.4). The method was validated in parameters of specificity, linearity, precision and accuracy. A plot of fluorescence intensity versus concentration of phenibut over the range of 800-1200 ng/ml is described by the regression equation y =0.1003x+1.4685 ( r =0.9999). The limit of detection and limit of quantification were 29.55 и 89.54 ng/ml respectively.https://www.pharmjournal.ru/jour/article/view/504fluorescence spectroscopydansyl chloridephenibuttablet drugvalidation |
| spellingShingle | O. I. Lazovskaya V. N. Leontiev E. V. Litvinova DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGS Разработка и регистрация лекарственных средств fluorescence spectroscopy dansyl chloride phenibut tablet drug validation |
| title | DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGS |
| title_full | DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGS |
| title_fullStr | DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGS |
| title_full_unstemmed | DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGS |
| title_short | DEVELOPMENT AND VALIDATION OF A SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF PHENIBUT IN TABLET DRUGS |
| title_sort | development and validation of a spectrofluorimetric method for the determination of phenibut in tablet drugs |
| topic | fluorescence spectroscopy dansyl chloride phenibut tablet drug validation |
| url | https://www.pharmjournal.ru/jour/article/view/504 |
| work_keys_str_mv | AT oilazovskaya developmentandvalidationofaspectrofluorimetricmethodforthedeterminationofphenibutintabletdrugs AT vnleontiev developmentandvalidationofaspectrofluorimetricmethodforthedeterminationofphenibutintabletdrugs AT evlitvinova developmentandvalidationofaspectrofluorimetricmethodforthedeterminationofphenibutintabletdrugs |