A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films
Purpose: This study aimed to develop an innovative, intelligent wound dressing capable of signaling infections through color changes. Design/methodology/approach: Using response surface methodology, the Rhodamine B fluorescence colorant was encapsulated within colloidal nanoparticles and integrated...
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Elsevier
2024-12-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024167011 |
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| author | Seyed Alireza Sheikholeslami Javad Esmaeili Saeedeh Zare Jalise Aboulfazl Barati |
| author_facet | Seyed Alireza Sheikholeslami Javad Esmaeili Saeedeh Zare Jalise Aboulfazl Barati |
| author_sort | Seyed Alireza Sheikholeslami |
| collection | DOAJ |
| description | Purpose: This study aimed to develop an innovative, intelligent wound dressing capable of signaling infections through color changes. Design/methodology/approach: Using response surface methodology, the Rhodamine B fluorescence colorant was encapsulated within colloidal nanoparticles and integrated into a sodium alginate patch at various concentrations. The physical and chemical characteristics of the nanoparticles and the wound dressing were thoroughly analyzed via dynamic light scattering (DLS), zeta potential measurements, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). Additionally, the biodegradability, hydrophilicity, swelling behavior, release kinetics, porosity, mechanical properties, biocompatibility, and infection detection capability of the wound dressing were evaluated. Findings: The results indicated that the average diameter of the synthesized colloidal nanoparticles was 300 nm before loading with Rhodamine B and increased to 400 nm after loading, with zeta potentials of 52 mV and −6 mV, respectively. The Rhodamine B-loaded wound dressing demonstrated adequate levels of swelling and hydrophilicity. Release studies revealed the gradual release of Rhodamine B at low pH. Cytotoxicity assays confirmed the high biocompatibility of the engineered wound dressing with the L929 cell line. Furthermore, bacterial exposure experiments indicated that the color change was activated in the presence of infection, making it visible under UV-A light. Originality/value: This research presents a novel approach to wound care by developing a smart wound dressing that can detect infections via color changes. These findings underscore the potential of this innovative wound dressing to improve infection management in clinical settings through its responsive and biocompatible design. |
| format | Article |
| id | doaj-art-52b3a697e917426ebc4336efdb098f62 |
| institution | OA Journals |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-52b3a697e917426ebc4336efdb098f622025-08-20T02:38:05ZengElsevierHeliyon2405-84402024-12-011023e4067010.1016/j.heliyon.2024.e40670A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based filmsSeyed Alireza Sheikholeslami0Javad Esmaeili1Saeedeh Zare Jalise2Aboulfazl Barati3Department of Chemical Engineering, Faculty of Engineering, Arak University, Arak, 38156-88349, IranDepartment of Tissue Engineering, TISSUEHUB Co., Tehran, Iran; Tissue Engineering Hub (TEHUB), Universal Scientific Education and Research Network (USERN), Tehran, Iran; Department of Applied Science, UQAC University, Quebec, CanadaDepartment of Tissue Engineering and Applied Cell Sciences, School of Medicine, Qom University of Medical Sciences, Qom, IranCollege of Arts and Sciences, University of Troy, Alabama, USA; Corresponding author. College of Arts and Sciences, University of Troy, Alabama, USAPurpose: This study aimed to develop an innovative, intelligent wound dressing capable of signaling infections through color changes. Design/methodology/approach: Using response surface methodology, the Rhodamine B fluorescence colorant was encapsulated within colloidal nanoparticles and integrated into a sodium alginate patch at various concentrations. The physical and chemical characteristics of the nanoparticles and the wound dressing were thoroughly analyzed via dynamic light scattering (DLS), zeta potential measurements, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). Additionally, the biodegradability, hydrophilicity, swelling behavior, release kinetics, porosity, mechanical properties, biocompatibility, and infection detection capability of the wound dressing were evaluated. Findings: The results indicated that the average diameter of the synthesized colloidal nanoparticles was 300 nm before loading with Rhodamine B and increased to 400 nm after loading, with zeta potentials of 52 mV and −6 mV, respectively. The Rhodamine B-loaded wound dressing demonstrated adequate levels of swelling and hydrophilicity. Release studies revealed the gradual release of Rhodamine B at low pH. Cytotoxicity assays confirmed the high biocompatibility of the engineered wound dressing with the L929 cell line. Furthermore, bacterial exposure experiments indicated that the color change was activated in the presence of infection, making it visible under UV-A light. Originality/value: This research presents a novel approach to wound care by developing a smart wound dressing that can detect infections via color changes. These findings underscore the potential of this innovative wound dressing to improve infection management in clinical settings through its responsive and biocompatible design.http://www.sciencedirect.com/science/article/pii/S2405844024167011NanoparticlesSmart wound dressingInfectionRhodamine BChitosan |
| spellingShingle | Seyed Alireza Sheikholeslami Javad Esmaeili Saeedeh Zare Jalise Aboulfazl Barati A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films Heliyon Nanoparticles Smart wound dressing Infection Rhodamine B Chitosan |
| title | A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films |
| title_full | A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films |
| title_fullStr | A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films |
| title_full_unstemmed | A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films |
| title_short | A response surface methodology study on the development of pH-sensitive wound dressings using Rhodamine B-loaded chitosan nanoparticles and sodium alginate-based films |
| title_sort | response surface methodology study on the development of ph sensitive wound dressings using rhodamine b loaded chitosan nanoparticles and sodium alginate based films |
| topic | Nanoparticles Smart wound dressing Infection Rhodamine B Chitosan |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024167011 |
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