Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats

Edible oil blending to formulate a health-protective fatty acid combination has opened new doors in food science. Material and methods: In this study, a 42-day biosafety assessment of a blended oil (BO) made from oils of coconut (CO), flaxseed (FO), extra virgin olive (EVOO), and sunflower (SFO) was...

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Main Authors: Roshina Rabail, Abdur Rauf Khalid, Amin Mousavi Khaneghah, Muhammad Faisal Manzoor, Gholamreza Abdi, Rana Muhammad Aadil
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Journal of Agriculture and Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666154325001395
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author Roshina Rabail
Abdur Rauf Khalid
Amin Mousavi Khaneghah
Muhammad Faisal Manzoor
Gholamreza Abdi
Rana Muhammad Aadil
author_facet Roshina Rabail
Abdur Rauf Khalid
Amin Mousavi Khaneghah
Muhammad Faisal Manzoor
Gholamreza Abdi
Rana Muhammad Aadil
author_sort Roshina Rabail
collection DOAJ
description Edible oil blending to formulate a health-protective fatty acid combination has opened new doors in food science. Material and methods: In this study, a 42-day biosafety assessment of a blended oil (BO) made from oils of coconut (CO), flaxseed (FO), extra virgin olive (EVOO), and sunflower (SFO) was performed on healthy rats. Rats were given these oils as 100 % fat replacement in control. Results: The results of BO in rats encouraged its health protection properties by significantly lowering body weight gain (68.8 ± 10.84), BMI (0.128 ± 0.01), body fat content (−0.41 ± 3.41), total cholesterol (43.00 ± 7.84), triglycerides (90.00 ± 9.00), total lipid contents (176.00 ± 24.39), and LDL (14.00 ± 4.12) while substantially improving HDL, LFTs, RFTs, and hematology when compared to single oils. BO consumption also indicated maximum protection against cardiovascular risk indices, including atherogenic (0.99 ± 0.16) and coronary (3.07 ± 0.21) indexes. Results for ECG also showed a normal cardiac rhythm in cardiac waves. Conclusion: Its regular consumption could help reduce the onset of obesity and metabolic diseases.
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spelling doaj-art-52b1bdd431cd4fa7b23ae5ba62bdfb952025-08-20T03:42:38ZengElsevierJournal of Agriculture and Food Research2666-15432025-04-012010176810.1016/j.jafr.2025.101768Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley ratsRoshina Rabail0Abdur Rauf Khalid1Amin Mousavi Khaneghah2Muhammad Faisal Manzoor3Gholamreza Abdi4Rana Muhammad Aadil5National Institute of Food Science and Technology, University of Agriculture, Faisalabad, 38000, PakistanDepartment of Livestock and Poultry Production, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, 60800, PakistanFaculty of Biotechnologies (BioTech), ITMO University, 9 Lomonosova Street, Saint Petersburg, 191002, Russia; Food and Drug Administration, Halal Research Center of IRI, Ministry of Health and Medical Education, Tehran, IranGuangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, China; Faculty of Sciences and Technology, ILMA University, Karachi, PakistanDepartment of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Bushehr, 75169, Iran; Corresponding author.National Institute of Food Science and Technology, University of Agriculture, Faisalabad, 38000, Pakistan; Corresponding author.Edible oil blending to formulate a health-protective fatty acid combination has opened new doors in food science. Material and methods: In this study, a 42-day biosafety assessment of a blended oil (BO) made from oils of coconut (CO), flaxseed (FO), extra virgin olive (EVOO), and sunflower (SFO) was performed on healthy rats. Rats were given these oils as 100 % fat replacement in control. Results: The results of BO in rats encouraged its health protection properties by significantly lowering body weight gain (68.8 ± 10.84), BMI (0.128 ± 0.01), body fat content (−0.41 ± 3.41), total cholesterol (43.00 ± 7.84), triglycerides (90.00 ± 9.00), total lipid contents (176.00 ± 24.39), and LDL (14.00 ± 4.12) while substantially improving HDL, LFTs, RFTs, and hematology when compared to single oils. BO consumption also indicated maximum protection against cardiovascular risk indices, including atherogenic (0.99 ± 0.16) and coronary (3.07 ± 0.21) indexes. Results for ECG also showed a normal cardiac rhythm in cardiac waves. Conclusion: Its regular consumption could help reduce the onset of obesity and metabolic diseases.http://www.sciencedirect.com/science/article/pii/S2666154325001395Blended cooking oilCardioprotective nutritional indicesCoconut oilExtra virgin olive oilFlaxseed oilSunflower oil
spellingShingle Roshina Rabail
Abdur Rauf Khalid
Amin Mousavi Khaneghah
Muhammad Faisal Manzoor
Gholamreza Abdi
Rana Muhammad Aadil
Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats
Journal of Agriculture and Food Research
Blended cooking oil
Cardioprotective nutritional indices
Coconut oil
Extra virgin olive oil
Flaxseed oil
Sunflower oil
title Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats
title_full Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats
title_fullStr Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats
title_full_unstemmed Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats
title_short Biosafety assessment of edible oil blended from flax, coconut, sunflower, and olive oils in healthy Sprague Dawley rats
title_sort biosafety assessment of edible oil blended from flax coconut sunflower and olive oils in healthy sprague dawley rats
topic Blended cooking oil
Cardioprotective nutritional indices
Coconut oil
Extra virgin olive oil
Flaxseed oil
Sunflower oil
url http://www.sciencedirect.com/science/article/pii/S2666154325001395
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