Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen

The objective of the study was to evaluate the effect of short (5 days) and long (9 days) SC protocols in ewes inseminated with frozen semen on the diameter of the uterine horn and pregnancy rate. The experiment was carried out at the Carolina Experimental Center, belonging to the National Universit...

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Main Authors: Yan Pierr Manrique Quispe, Uri Harold Pérez Guerra, Julio Málaga Apaza, Wilbur Rubén Ayma Flores, Oscar Efrain Cardenas Minaya, Manuel Guido Pérez Durand
Format: Article
Language:English
Published: Selva Andina Research Society 2021-10-01
Series:Journal of the Selva Andina Animal Science
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Online Access:http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2311-25812021000200073&lng=es&nrm=iso&tlng=en
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author Yan Pierr Manrique Quispe
Uri Harold Pérez Guerra
Julio Málaga Apaza
Wilbur Rubén Ayma Flores
Oscar Efrain Cardenas Minaya
Manuel Guido Pérez Durand
author_facet Yan Pierr Manrique Quispe
Uri Harold Pérez Guerra
Julio Málaga Apaza
Wilbur Rubén Ayma Flores
Oscar Efrain Cardenas Minaya
Manuel Guido Pérez Durand
author_sort Yan Pierr Manrique Quispe
collection DOAJ
description The objective of the study was to evaluate the effect of short (5 days) and long (9 days) SC protocols in ewes inseminated with frozen semen on the diameter of the uterine horn and pregnancy rate. The experiment was carried out at the Carolina Experimental Center, belonging to the National University of the Altiplano, and 40 ewes between Corriedale and Criollas were used; in groups of 20 for each treatment, which were synchronized with intravaginal sponges based on Medroxyprogesterone acetate, for 9 days (long protocol) and 5 days (short protocol) and at the time of sponge removal, 350 IU of eCG were applied. To measure the diameter of the uterine horn, ultrasonography was performed at 36 h post sponge removal and at 56 h after cervical insemination with thawed semen and a subsequent ultrasonography at 35 days to confirm gestation. Quantitative data were analyzed using the statistical t-test for uterine horn diameter and Chi-square for gestation rate. The results for uterine horn diameter were 11.11±1.52 and 12.12±2.12 mm for the short and long synchronization protocol, respectively. Regarding the pregnancy rate, 21.05 % and 25 % were obtained for the short and long synchronization protocol, respectively. Therefore, we can conclude that the long progesterone treatment had better results than the short treatment both in uterine diameter and pregnancy rate, but this difference was not significant (p>0.05).
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spelling doaj-art-52b0fdab72eb4e94b4543ae6cd07f7342025-08-20T03:59:44ZengSelva Andina Research SocietyJournal of the Selva Andina Animal Science2311-37662311-25812021-10-01827381doi:10.36610/j.jsaas.2021.080200073Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semenYan Pierr Manrique Quispe0https://orcid.org/0000-0001-8448-3174Uri Harold Pérez Guerra1https://orcid.org/0000-0001-8321-8651Julio Málaga Apaza2https://orcid.org/0000-0003-4942-0041Wilbur Rubén Ayma Flores3https://orcid.org/0000-0001-8162-9414Oscar Efrain Cardenas Minaya4https://orcid.org/0000-0003-2138-9863Manuel Guido Pérez Durand5https://orcid.org/0000-0001-6544-2924Universidad Nacional del Altiplano Puno. Facultad de Medicina Veterinaria y Zootecnia.. Laboratorio de Reproducción Animal. Av. Panamá No 710, Puno, Perú. Tel: +051-599430.Universidad Nacional del Altiplano Puno. Facultad de Medicina Veterinaria y Zootecnia.. Laboratorio de Reproducción Animal. Av. Panamá No 710, Puno, Perú. Tel: +051-599430.Universidad Nacional del Altiplano Puno. Facultad de Medicina Veterinaria y Zootecnia. Dirección de Post Grado. Av. Floral Nº 1153. Puno, Perú. Tel: +051-599430Universidad Nacional del Altiplano Puno. Facultad de Medicina Veterinaria y Zootecnia. Centro Experimental Carolina. Av. Floral Nº 1153. Puno, Perú. Tel: +051-599430Instituto Nacional de Innovación Agraria. Laboratorio de Biotecnología Reproductiva La rinconada - Salcedo s/n. Puno, Perú. Tel: +051-363812Universidad Nacional del Altiplano Puno. Facultad de Medicina Veterinaria y Zootecnia.. Laboratorio de Reproducción Animal. Av. Panamá No 710, Puno, Perú. Tel: +051-599430.The objective of the study was to evaluate the effect of short (5 days) and long (9 days) SC protocols in ewes inseminated with frozen semen on the diameter of the uterine horn and pregnancy rate. The experiment was carried out at the Carolina Experimental Center, belonging to the National University of the Altiplano, and 40 ewes between Corriedale and Criollas were used; in groups of 20 for each treatment, which were synchronized with intravaginal sponges based on Medroxyprogesterone acetate, for 9 days (long protocol) and 5 days (short protocol) and at the time of sponge removal, 350 IU of eCG were applied. To measure the diameter of the uterine horn, ultrasonography was performed at 36 h post sponge removal and at 56 h after cervical insemination with thawed semen and a subsequent ultrasonography at 35 days to confirm gestation. Quantitative data were analyzed using the statistical t-test for uterine horn diameter and Chi-square for gestation rate. The results for uterine horn diameter were 11.11±1.52 and 12.12±2.12 mm for the short and long synchronization protocol, respectively. Regarding the pregnancy rate, 21.05 % and 25 % were obtained for the short and long synchronization protocol, respectively. Therefore, we can conclude that the long progesterone treatment had better results than the short treatment both in uterine diameter and pregnancy rate, but this difference was not significant (p>0.05).http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2311-25812021000200073&lng=es&nrm=iso&tlng=enestrus; fertility; ovine; pregnancy; thawed semen; uterine diameter.
spellingShingle Yan Pierr Manrique Quispe
Uri Harold Pérez Guerra
Julio Málaga Apaza
Wilbur Rubén Ayma Flores
Oscar Efrain Cardenas Minaya
Manuel Guido Pérez Durand
Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen
Journal of the Selva Andina Animal Science
estrus; fertility; ovine; pregnancy; thawed semen; uterine diameter.
title Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen
title_full Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen
title_fullStr Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen
title_full_unstemmed Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen
title_short Evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen
title_sort evaluation of the short and long estrus synchronization protocol in inseminated sheep with frozen semen
topic estrus; fertility; ovine; pregnancy; thawed semen; uterine diameter.
url http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S2311-25812021000200073&lng=es&nrm=iso&tlng=en
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