Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk
This study investigated the impact of the addition of 5 mM trisodium citrate (TSC) or disodium hydrogen phosphate (DSHP) and heat treatment (85 or 95 °C for 5 min) on the in vitro gastrointestinal digestibility of proteins in blends (0:100, 25:75, 50:50, 75:25, 100:0) of skimmed buffalo and bovine m...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Food Chemistry Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X24001758 |
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| author | Carolyn T. Mejares Thom Huppertz Jayani Chandrapala |
| author_facet | Carolyn T. Mejares Thom Huppertz Jayani Chandrapala |
| author_sort | Carolyn T. Mejares |
| collection | DOAJ |
| description | This study investigated the impact of the addition of 5 mM trisodium citrate (TSC) or disodium hydrogen phosphate (DSHP) and heat treatment (85 or 95 °C for 5 min) on the in vitro gastrointestinal digestibility of proteins in blends (0:100, 25:75, 50:50, 75:25, 100:0) of skimmed buffalo and bovine milk. Digestibility of proteins decreased with increasing proportion of buffalo skim milk, irrespective of heat treatment and calcium sequestering salt (CSS) addition. Heat treatment alone did not induce significant changes in the digestibility of proteins. CSS addition and heat treatment did not significantly affect whey protein digestibility but impacted the digestibility of κ-casein. While addition of TSC and DSHP induced comparable κ-casein digestibility in bovine skim milk and milk blends, the addition of TSC and heat treatment of buffalo skim milk effected maximum κ-casein gastrointestinal digestibility. Overall, α-lactalbumin and κ-casein were the most resistant proteins against degradation by digestive enzymes. These results may be useful in deciding appropriate process intervention that could maximise gastrointestinal digestion of proteins in buffalo and bovine milk blends. |
| format | Article |
| id | doaj-art-52afa9ece41e4b3fbe95e1532dcc301c |
| institution | DOAJ |
| issn | 2772-753X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj-art-52afa9ece41e4b3fbe95e1532dcc301c2025-08-20T02:50:16ZengElsevierFood Chemistry Advances2772-753X2024-12-01510077910.1016/j.focha.2024.100779Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milkCarolyn T. Mejares0Thom Huppertz1Jayani Chandrapala2School of Science, STEM College, RMIT University, Melbourne, VIC 3083, Australia; School of Technology, University of the Philippines Visayas, Miagao, Iloilo 5023 PhilippinesFrieslandCampina, Amersfoort, the Netherlands; Food Quality and Design Group, Wageningen University & Research, Wageningen, the NetherlandsSchool of Science, STEM College, RMIT University, Melbourne, VIC 3083, Australia; Corresponding author.This study investigated the impact of the addition of 5 mM trisodium citrate (TSC) or disodium hydrogen phosphate (DSHP) and heat treatment (85 or 95 °C for 5 min) on the in vitro gastrointestinal digestibility of proteins in blends (0:100, 25:75, 50:50, 75:25, 100:0) of skimmed buffalo and bovine milk. Digestibility of proteins decreased with increasing proportion of buffalo skim milk, irrespective of heat treatment and calcium sequestering salt (CSS) addition. Heat treatment alone did not induce significant changes in the digestibility of proteins. CSS addition and heat treatment did not significantly affect whey protein digestibility but impacted the digestibility of κ-casein. While addition of TSC and DSHP induced comparable κ-casein digestibility in bovine skim milk and milk blends, the addition of TSC and heat treatment of buffalo skim milk effected maximum κ-casein gastrointestinal digestibility. Overall, α-lactalbumin and κ-casein were the most resistant proteins against degradation by digestive enzymes. These results may be useful in deciding appropriate process intervention that could maximise gastrointestinal digestion of proteins in buffalo and bovine milk blends.http://www.sciencedirect.com/science/article/pii/S2772753X24001758Buffalo milkMilk mixtureHeat treatmentIn vitro digestionCalcium sequestering salt |
| spellingShingle | Carolyn T. Mejares Thom Huppertz Jayani Chandrapala Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk Food Chemistry Advances Buffalo milk Milk mixture Heat treatment In vitro digestion Calcium sequestering salt |
| title | Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk |
| title_full | Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk |
| title_fullStr | Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk |
| title_full_unstemmed | Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk |
| title_short | Influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk |
| title_sort | influence of heat treatment and calcium sequestering salts on in vitro protein digestion in blends of skimmed buffalo and bovine milk |
| topic | Buffalo milk Milk mixture Heat treatment In vitro digestion Calcium sequestering salt |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X24001758 |
| work_keys_str_mv | AT carolyntmejares influenceofheattreatmentandcalciumsequesteringsaltsoninvitroproteindigestioninblendsofskimmedbuffaloandbovinemilk AT thomhuppertz influenceofheattreatmentandcalciumsequesteringsaltsoninvitroproteindigestioninblendsofskimmedbuffaloandbovinemilk AT jayanichandrapala influenceofheattreatmentandcalciumsequesteringsaltsoninvitroproteindigestioninblendsofskimmedbuffaloandbovinemilk |