The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.

ABSTRACT    Target production of catfish in aquaculture can be reached by suppressing the pathogenic bacterial infection. Previous works had shown that turmeric Curcuma longa has antibacterial activity. The objectives of this research were to determine the best method of extraction and to evaluate t...

Full description

Saved in:
Bibliographic Details
Main Authors: Dinamella Wahjuningrum, Muharram Nur Ikhsan, , Sukenda, Yan Evan
Format: Article
Language:English
Published: Asosiasi Sains Akuakultur Indonesia 2015-05-01
Series:Jurnal Akuakultur Indonesia
Online Access:https://journal.ipb.ac.id/index.php/jai/article/view/9443
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832587068529704960
author Dinamella Wahjuningrum
Muharram Nur Ikhsan
, Sukenda
Yan Evan
author_facet Dinamella Wahjuningrum
Muharram Nur Ikhsan
, Sukenda
Yan Evan
author_sort Dinamella Wahjuningrum
collection DOAJ
description ABSTRACT    Target production of catfish in aquaculture can be reached by suppressing the pathogenic bacterial infection. Previous works had shown that turmeric Curcuma longa has antibacterial activity. The objectives of this research were to determine the best method of extraction and to evaluate the efficacy of turmeric extract as feed additive to control Edwardsiella tarda disease in catfish culture. Briefly, the objective was achieved through in vitro assay based on inhibition ability of extraction method against E. tarda, while the following objective was obtained through in vivo assay based on their survival during challenge test either as preventive or curative measurement. A complete randomized design with three replications was used for each assay. Furthermore, challenge test was done by mean intraperitoneal injection at concentration 106 cfu/mL of E. tarda (LD50). The results showed that 15 minutes decoction method allowed the best inhibition with diameter 7.42 mm of clear zone and then curative measurement using turmeric extract could be the best application against E. tarda since it gave 86.67% of survival. Clinical signs such as swelling, hemoraghic, body ulcer, gastroentritis and gaseous captivity were observed on challenged fish. However, there were no significant different among treatments for specific growth, body weight, and absolute length parameters.    Keywords: Edwardsiella tarda, extraction, turmeric, catfish, inhibition zone     ABSTRAK   Peningkatan produksi ikan lele dapat dicapai melalui pencegahan infeksi penyakit bakterial. Hasil beberapa penelitian membuktikan bahwa kunyit Curcuma longa terbukti memiliki zat aktif yang bersifat antibakteri. Penelitian ini bertujuan untuk mencari metode ekstraksi terbaik dan mengevaluasi efektivitas penambahan ekstrak kunyit pada pakan untuk pengendalian patogen Edwardsiella tarda pada ikan lele. Metoda ekstraksi kunyit diuji secara in vitro dengan metoda zona hambat, sedangkan efikasi diuji secara in vivo melalui perlakuan pencegahan dan pengobatan. Penelitian didesain dalam rancangan acak lengkap dengan tiga ulangan. Efikasi ekstrak kunyit diketahui dari nilai sintasan ikan lele hasil uji tantang E. Tarda melalui injeksi peritoneal dengan dosis 106 cfu/ mL (LD50). Hasil uji in vitro menunjukkan bahwa metode dekoksi selama 15 menit memberikan zona hambat terbaik yaitu sebesar 7,42 mm. Hasil uji in vivo menunjukkan bahwa ekstrak kunyit sebagai tindak pengobatan memberikan nilai sintasan terbaik, yaitu sebesar 86,67%. Ikan lele yang diuji tantang menunjukkan gejala klinis berupa pembengkakan, luka, gastroentritis, dan gas pada perut. Tidak terdapat perbedaan nyata di antara perlakuan untuk parameter laju pertumbuhan harian, bobot harian, dan pertumbuhan panjang mutlak.   Kata kunci: Edwardsiella tarda, ekstraksi, kunyit, ikan lele, zona hambat
format Article
id doaj-art-529e32dbb834465ca3144ef50ec48844
institution Kabale University
issn 1412-5269
2354-6700
language English
publishDate 2015-05-01
publisher Asosiasi Sains Akuakultur Indonesia
record_format Article
series Jurnal Akuakultur Indonesia
spelling doaj-art-529e32dbb834465ca3144ef50ec488442025-01-24T17:59:08ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002015-05-0113110.19027/jai.13.1-107889The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.Dinamella Wahjuningrum0Muharram Nur Ikhsan1, Sukenda2Yan Evan3Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Dramaga Bogor 16680Loka Pemeriksaan Penyakit Ikan dan Lingkungan Serang, Banten Jl. Raya Carita Ds. Umbul Tanjung Kec. Cinangka, Anyer Lor Serang, Banten 42167ABSTRACT    Target production of catfish in aquaculture can be reached by suppressing the pathogenic bacterial infection. Previous works had shown that turmeric Curcuma longa has antibacterial activity. The objectives of this research were to determine the best method of extraction and to evaluate the efficacy of turmeric extract as feed additive to control Edwardsiella tarda disease in catfish culture. Briefly, the objective was achieved through in vitro assay based on inhibition ability of extraction method against E. tarda, while the following objective was obtained through in vivo assay based on their survival during challenge test either as preventive or curative measurement. A complete randomized design with three replications was used for each assay. Furthermore, challenge test was done by mean intraperitoneal injection at concentration 106 cfu/mL of E. tarda (LD50). The results showed that 15 minutes decoction method allowed the best inhibition with diameter 7.42 mm of clear zone and then curative measurement using turmeric extract could be the best application against E. tarda since it gave 86.67% of survival. Clinical signs such as swelling, hemoraghic, body ulcer, gastroentritis and gaseous captivity were observed on challenged fish. However, there were no significant different among treatments for specific growth, body weight, and absolute length parameters.    Keywords: Edwardsiella tarda, extraction, turmeric, catfish, inhibition zone     ABSTRAK   Peningkatan produksi ikan lele dapat dicapai melalui pencegahan infeksi penyakit bakterial. Hasil beberapa penelitian membuktikan bahwa kunyit Curcuma longa terbukti memiliki zat aktif yang bersifat antibakteri. Penelitian ini bertujuan untuk mencari metode ekstraksi terbaik dan mengevaluasi efektivitas penambahan ekstrak kunyit pada pakan untuk pengendalian patogen Edwardsiella tarda pada ikan lele. Metoda ekstraksi kunyit diuji secara in vitro dengan metoda zona hambat, sedangkan efikasi diuji secara in vivo melalui perlakuan pencegahan dan pengobatan. Penelitian didesain dalam rancangan acak lengkap dengan tiga ulangan. Efikasi ekstrak kunyit diketahui dari nilai sintasan ikan lele hasil uji tantang E. Tarda melalui injeksi peritoneal dengan dosis 106 cfu/ mL (LD50). Hasil uji in vitro menunjukkan bahwa metode dekoksi selama 15 menit memberikan zona hambat terbaik yaitu sebesar 7,42 mm. Hasil uji in vivo menunjukkan bahwa ekstrak kunyit sebagai tindak pengobatan memberikan nilai sintasan terbaik, yaitu sebesar 86,67%. Ikan lele yang diuji tantang menunjukkan gejala klinis berupa pembengkakan, luka, gastroentritis, dan gas pada perut. Tidak terdapat perbedaan nyata di antara perlakuan untuk parameter laju pertumbuhan harian, bobot harian, dan pertumbuhan panjang mutlak.   Kata kunci: Edwardsiella tarda, ekstraksi, kunyit, ikan lele, zona hambathttps://journal.ipb.ac.id/index.php/jai/article/view/9443
spellingShingle Dinamella Wahjuningrum
Muharram Nur Ikhsan
, Sukenda
Yan Evan
The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.
Jurnal Akuakultur Indonesia
title The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.
title_full The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.
title_fullStr The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.
title_full_unstemmed The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.
title_short The use of Curcuma longa extract to control Edwardsiella tarda infection on Clarias sp.
title_sort use of curcuma longa extract to control edwardsiella tarda infection on clarias sp
url https://journal.ipb.ac.id/index.php/jai/article/view/9443
work_keys_str_mv AT dinamellawahjuningrum theuseofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp
AT muharramnurikhsan theuseofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp
AT sukenda theuseofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp
AT yanevan theuseofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp
AT dinamellawahjuningrum useofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp
AT muharramnurikhsan useofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp
AT sukenda useofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp
AT yanevan useofcurcumalongaextracttocontroledwardsiellatardainfectiononclariassp