Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic Cats

ABSTRACT Background The FreeStyle Libre 3 (FSL3) has several improvements compared to previous FreeStyle Libre systems, but its accuracy has not yet been determined in cats. In diabetic people, FSL3 offers increased accuracy, and its smaller size could be advantageous for use in veterinary patients....

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Main Authors: Antonio M. Tardo, Chiquitha Crews, Jocelyn Mott, Lauren T. Porter, Christopher Adin, Chen Gilor
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Journal of Veterinary Internal Medicine
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Online Access:https://doi.org/10.1111/jvim.70048
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author Antonio M. Tardo
Chiquitha Crews
Jocelyn Mott
Lauren T. Porter
Christopher Adin
Chen Gilor
author_facet Antonio M. Tardo
Chiquitha Crews
Jocelyn Mott
Lauren T. Porter
Christopher Adin
Chen Gilor
author_sort Antonio M. Tardo
collection DOAJ
description ABSTRACT Background The FreeStyle Libre 3 (FSL3) has several improvements compared to previous FreeStyle Libre systems, but its accuracy has not yet been determined in cats. In diabetic people, FSL3 offers increased accuracy, and its smaller size could be advantageous for use in veterinary patients. Objectives Assess the accuracy of FSL3 in cats with experimentally induced hypoglycemia. Animals Seven healthy, purpose‐bred cats. Methods Hyperinsulinemic‐hypoglycemic clamps were performed. Interstitial glucose concentration (IG), measured by FSL3, was compared to blood glucose concentration (BG) measured by AlphaTrak2. Data were analyzed for all paired measurements (n = 474) and during stable BG (≤ 1 mg/dL/min change over 10 min). Pearson's r test, Bland–Altman test, and Parkes Error Grid analysis (EGA) respectively used to determine correlation, bias, and clinical accuracy. Results Blood glucose concentration and IG correlated strongly (r = 0.86, p < 0.0001) in stable glycemia and moderately at all rates of change (r = 0.73, p < 0.0001). Analytical accuracy was not achieved, whereas clinical accuracy was demonstrated with 99%–100% of the results in zones A + B of the Parkes EGA. Interstitial glucose concentration underestimated BG in euglycemia and mild hypoglycemia (mean −11.7 ± 11.2, −5.5 ± 9.1, −1.5 ± 6.0 mg/dL in the ranges 91–120, 66–90, and 56–65 mg/dL, respectively), but overestimated BG in marked hypoglycemia (mean 6.3 ± 5.7, 15.7 ± 5.6 mg/dL in the ranges 46–55 and < 45 mg/dL, respectively). Conclusions The FSL3 underestimates BG across most of the hypo‐euglycemic range but overestimates BG in marked hypoglycemia (< 55 mg/dL). Recognizing the proportional, glycemic‐dependent bias of FSL3 improves the safety of its clinical application in cats.
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spelling doaj-art-529807cdb2e042fc9e730fbd98965c982025-08-20T02:14:23ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-03-01392n/an/a10.1111/jvim.70048Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic CatsAntonio M. Tardo0Chiquitha Crews1Jocelyn Mott2Lauren T. Porter3Christopher Adin4Chen Gilor5Department of Veterinary Medical Sciences University of Bologna Ozzano dell'Emilia ItalyDepartment of Small Animal Clinical Sciences University of Florida, College of Veterinary Medicine Gainesville Florida USADepartment of Small Animal Clinical Sciences University of Florida, College of Veterinary Medicine Gainesville Florida USADepartment of Small Animal Clinical Sciences University of Florida, College of Veterinary Medicine Gainesville Florida USADepartment of Small Animal Clinical Sciences University of Florida, College of Veterinary Medicine Gainesville Florida USADepartment of Small Animal Clinical Sciences University of Florida, College of Veterinary Medicine Gainesville Florida USAABSTRACT Background The FreeStyle Libre 3 (FSL3) has several improvements compared to previous FreeStyle Libre systems, but its accuracy has not yet been determined in cats. In diabetic people, FSL3 offers increased accuracy, and its smaller size could be advantageous for use in veterinary patients. Objectives Assess the accuracy of FSL3 in cats with experimentally induced hypoglycemia. Animals Seven healthy, purpose‐bred cats. Methods Hyperinsulinemic‐hypoglycemic clamps were performed. Interstitial glucose concentration (IG), measured by FSL3, was compared to blood glucose concentration (BG) measured by AlphaTrak2. Data were analyzed for all paired measurements (n = 474) and during stable BG (≤ 1 mg/dL/min change over 10 min). Pearson's r test, Bland–Altman test, and Parkes Error Grid analysis (EGA) respectively used to determine correlation, bias, and clinical accuracy. Results Blood glucose concentration and IG correlated strongly (r = 0.86, p < 0.0001) in stable glycemia and moderately at all rates of change (r = 0.73, p < 0.0001). Analytical accuracy was not achieved, whereas clinical accuracy was demonstrated with 99%–100% of the results in zones A + B of the Parkes EGA. Interstitial glucose concentration underestimated BG in euglycemia and mild hypoglycemia (mean −11.7 ± 11.2, −5.5 ± 9.1, −1.5 ± 6.0 mg/dL in the ranges 91–120, 66–90, and 56–65 mg/dL, respectively), but overestimated BG in marked hypoglycemia (mean 6.3 ± 5.7, 15.7 ± 5.6 mg/dL in the ranges 46–55 and < 45 mg/dL, respectively). Conclusions The FSL3 underestimates BG across most of the hypo‐euglycemic range but overestimates BG in marked hypoglycemia (< 55 mg/dL). Recognizing the proportional, glycemic‐dependent bias of FSL3 improves the safety of its clinical application in cats.https://doi.org/10.1111/jvim.70048diabetes mellitusflash glucose monitoring systemhypoglycemiainsulininterstitial glucose
spellingShingle Antonio M. Tardo
Chiquitha Crews
Jocelyn Mott
Lauren T. Porter
Christopher Adin
Chen Gilor
Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic Cats
Journal of Veterinary Internal Medicine
diabetes mellitus
flash glucose monitoring system
hypoglycemia
insulin
interstitial glucose
title Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic Cats
title_full Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic Cats
title_fullStr Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic Cats
title_full_unstemmed Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic Cats
title_short Accuracy of the Freestyle Libre 3 Continuous Glucose Monitoring System in Hypo‐ and Euglycemic Cats
title_sort accuracy of the freestyle libre 3 continuous glucose monitoring system in hypo and euglycemic cats
topic diabetes mellitus
flash glucose monitoring system
hypoglycemia
insulin
interstitial glucose
url https://doi.org/10.1111/jvim.70048
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