Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting
Abstract Spontaneous mutations play a key role in plant evolution. In this study, we identified and characterized albino rice mutants in an F₂ population derived from a cross between the japonica elite line Hwaseong and the introgression line CR5029, which carries chromosome segments from Oryza gran...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-12931-6 |
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| author | Kyu-Chan Shim Yun-A Jeon Hyun-Sook Lee Sang-Nag Ahn Inkyu Park |
| author_facet | Kyu-Chan Shim Yun-A Jeon Hyun-Sook Lee Sang-Nag Ahn Inkyu Park |
| author_sort | Kyu-Chan Shim |
| collection | DOAJ |
| description | Abstract Spontaneous mutations play a key role in plant evolution. In this study, we identified and characterized albino rice mutants in an F₂ population derived from a cross between the japonica elite line Hwaseong and the introgression line CR5029, which carries chromosome segments from Oryza grandiglumis. Among 440 F₂ plants, ten exhibited an albino phenotype after transplantation, which was not observed in the parental lines, suggesting that either novel allelic combinations or spontaneous mutations may be responsible. QTL-seq analysis identified a major locus, qALB11, on chromosome 11, with a 1-bp T deletion in the Os11g0490800 (OsABCI8) gene as the most likely causal mutation. This deletion resulted in a truncated protein lacking the ABC domain, potentially affecting its function. Protein structure modeling confirmed structural differences between the mutant and wild-type proteins. Further genome-wide screening identified 669 spontaneous variants in the progeny population, many of which were associated with repeat sequences. These findings not only elucidate the genetic basis of a conditional albino phenotype but also highlight the broader role of spontaneous mutations and genome instability in plastid development and environmental stress adaptation in rice. |
| format | Article |
| id | doaj-art-529697433e034bdebabf1376ef776ce8 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-529697433e034bdebabf1376ef776ce82025-08-20T03:45:55ZengNature PortfolioScientific Reports2045-23222025-07-0115111010.1038/s41598-025-12931-6Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplantingKyu-Chan Shim0Yun-A Jeon1Hyun-Sook Lee2Sang-Nag Ahn3Inkyu Park4Department of Crop Science, College of Agriculture and Life Science, Chungnam National UniversityDepartment of Crop Science, College of Agriculture and Life Science, Chungnam National UniversityDepartment of Crop Science, College of Agriculture and Life Science, Chungnam National UniversityDepartment of Crop Science, College of Agriculture and Life Science, Chungnam National UniversityDepartment of Biology, Changwon National UniversityAbstract Spontaneous mutations play a key role in plant evolution. In this study, we identified and characterized albino rice mutants in an F₂ population derived from a cross between the japonica elite line Hwaseong and the introgression line CR5029, which carries chromosome segments from Oryza grandiglumis. Among 440 F₂ plants, ten exhibited an albino phenotype after transplantation, which was not observed in the parental lines, suggesting that either novel allelic combinations or spontaneous mutations may be responsible. QTL-seq analysis identified a major locus, qALB11, on chromosome 11, with a 1-bp T deletion in the Os11g0490800 (OsABCI8) gene as the most likely causal mutation. This deletion resulted in a truncated protein lacking the ABC domain, potentially affecting its function. Protein structure modeling confirmed structural differences between the mutant and wild-type proteins. Further genome-wide screening identified 669 spontaneous variants in the progeny population, many of which were associated with repeat sequences. These findings not only elucidate the genetic basis of a conditional albino phenotype but also highlight the broader role of spontaneous mutations and genome instability in plastid development and environmental stress adaptation in rice.https://doi.org/10.1038/s41598-025-12931-6RiceSpontaneous mutationAlbinoTransplanting |
| spellingShingle | Kyu-Chan Shim Yun-A Jeon Hyun-Sook Lee Sang-Nag Ahn Inkyu Park Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting Scientific Reports Rice Spontaneous mutation Albino Transplanting |
| title | Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting |
| title_full | Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting |
| title_fullStr | Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting |
| title_full_unstemmed | Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting |
| title_short | Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting |
| title_sort | spontaneous mutation in osabci8 caused an albino rice mutant induced by transplanting |
| topic | Rice Spontaneous mutation Albino Transplanting |
| url | https://doi.org/10.1038/s41598-025-12931-6 |
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