Spontaneous mutation in OsABCI8 caused an albino rice mutant induced by transplanting
Abstract Spontaneous mutations play a key role in plant evolution. In this study, we identified and characterized albino rice mutants in an F₂ population derived from a cross between the japonica elite line Hwaseong and the introgression line CR5029, which carries chromosome segments from Oryza gran...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-12931-6 |
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| Summary: | Abstract Spontaneous mutations play a key role in plant evolution. In this study, we identified and characterized albino rice mutants in an F₂ population derived from a cross between the japonica elite line Hwaseong and the introgression line CR5029, which carries chromosome segments from Oryza grandiglumis. Among 440 F₂ plants, ten exhibited an albino phenotype after transplantation, which was not observed in the parental lines, suggesting that either novel allelic combinations or spontaneous mutations may be responsible. QTL-seq analysis identified a major locus, qALB11, on chromosome 11, with a 1-bp T deletion in the Os11g0490800 (OsABCI8) gene as the most likely causal mutation. This deletion resulted in a truncated protein lacking the ABC domain, potentially affecting its function. Protein structure modeling confirmed structural differences between the mutant and wild-type proteins. Further genome-wide screening identified 669 spontaneous variants in the progeny population, many of which were associated with repeat sequences. These findings not only elucidate the genetic basis of a conditional albino phenotype but also highlight the broader role of spontaneous mutations and genome instability in plastid development and environmental stress adaptation in rice. |
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| ISSN: | 2045-2322 |