Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease
Our study was aimed at analysis of expression of genes controlling individual stages of an inflammatory process in combined therapy of chronic obstructive lung disease (COPD). Evaluation of activity of GR, COX-2, and MMP1 genes in blood leukocytes was determined in forty one patients with COPD exace...
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| Format: | Article |
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Academician I.P. Pavlov First St. Petersburg State Medical University
2015-03-01
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| Series: | Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова |
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| Online Access: | https://www.sci-notes.ru/jour/article/view/108 |
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| author | N. D. Yelshin A. B. Chukhlovin N. A. Kuzubova I. A. Shahanova O. N. Titova |
| author_facet | N. D. Yelshin A. B. Chukhlovin N. A. Kuzubova I. A. Shahanova O. N. Titova |
| author_sort | N. D. Yelshin |
| collection | DOAJ |
| description | Our study was aimed at analysis of expression of genes controlling individual stages of an inflammatory process in combined therapy of chronic obstructive lung disease (COPD). Evaluation of activity of GR, COX-2, and MMP1 genes in blood leukocytes was determined in forty one patients with COPD exacerbations (GOLD stage II to III, Group D) who underwent a two-week combined treatment including Tiotropium Bromide, Formoterol, and glucocotricoid inhalations, as well as parenteral antibacterial treatment. Before and after treatment, relative content of specific mRNAs for GR, GC receptors, and matrix metalloproteinase-1 (COX-2, GR, and MMP1) genes were determined with real-time PCR. Mean values of the gene expression did not significantly differ between the pre- and post-treatment time points. We have found, however, a highly significant correlation between individual changes of the СОХ2, ММР1 и GR gene expression, as well as reverse correlation between СОХ2, GR expression and positive changes in C-reactive protein levels following treatment course. The results of study confirm some interrelations between changes in expression of the inflammation-modulating genes and improvement of clinical parameters after combined drug therapy of the COPD. |
| format | Article |
| id | doaj-art-527a69001c07462e829ec825b9600607 |
| institution | Kabale University |
| issn | 1607-4181 2541-8807 |
| language | Russian |
| publishDate | 2015-03-01 |
| publisher | Academician I.P. Pavlov First St. Petersburg State Medical University |
| record_format | Article |
| series | Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова |
| spelling | doaj-art-527a69001c07462e829ec825b96006072025-08-20T03:59:57ZrusAcademician I.P. Pavlov First St. Petersburg State Medical UniversityУчёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова1607-41812541-88072015-03-01221343710.24884/1607-4181-2015-22-1-34-37104Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung diseaseN. D. Yelshin0A. B. Chukhlovin1N. A. Kuzubova2I. A. Shahanova3O. N. Titova4Первый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаOur study was aimed at analysis of expression of genes controlling individual stages of an inflammatory process in combined therapy of chronic obstructive lung disease (COPD). Evaluation of activity of GR, COX-2, and MMP1 genes in blood leukocytes was determined in forty one patients with COPD exacerbations (GOLD stage II to III, Group D) who underwent a two-week combined treatment including Tiotropium Bromide, Formoterol, and glucocotricoid inhalations, as well as parenteral antibacterial treatment. Before and after treatment, relative content of specific mRNAs for GR, GC receptors, and matrix metalloproteinase-1 (COX-2, GR, and MMP1) genes were determined with real-time PCR. Mean values of the gene expression did not significantly differ between the pre- and post-treatment time points. We have found, however, a highly significant correlation between individual changes of the СОХ2, ММР1 и GR gene expression, as well as reverse correlation between СОХ2, GR expression and positive changes in C-reactive protein levels following treatment course. The results of study confirm some interrelations between changes in expression of the inflammation-modulating genes and improvement of clinical parameters after combined drug therapy of the COPD.https://www.sci-notes.ru/jour/article/view/108хроническая обструктивная болезнь легкихкомплексная терапиялейкоцитыэкспрессия геновсох2ммр1grchronic obstructive lung diseasecombined therapyleukocytesgene expressionсох2ммр1gr |
| spellingShingle | N. D. Yelshin A. B. Chukhlovin N. A. Kuzubova I. A. Shahanova O. N. Titova Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова хроническая обструктивная болезнь легких комплексная терапия лейкоциты экспрессия генов сох2 ммр1 gr chronic obstructive lung disease combined therapy leukocytes gene expression сох2 ммр1 gr |
| title | Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease |
| title_full | Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease |
| title_fullStr | Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease |
| title_full_unstemmed | Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease |
| title_short | Expression of GR, COX2и MMP1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease |
| title_sort | expression of gr cox2и mmp1 genes in peripheral blood leukocytes after intensive therapy of the patients with chronic obstructive lung disease |
| topic | хроническая обструктивная болезнь легких комплексная терапия лейкоциты экспрессия генов сох2 ммр1 gr chronic obstructive lung disease combined therapy leukocytes gene expression сох2 ммр1 gr |
| url | https://www.sci-notes.ru/jour/article/view/108 |
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