Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice
Aims. Diabetes is a proinflammatory state, evidenced by increased pattern recognition receptors and the inflammasome (NOD-like receptor family pyrin domain (NLRP)) complex. Recent reports have elucidated the role of the gut microbiome in diabetes, but there is limited data on the gut microbiome in N...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/6519785 |
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| author | Roma Pahwa Miriam Balderas Ishwarlal Jialal Xinpu Chen Ruth Ann Luna Sridevi Devaraj |
| author_facet | Roma Pahwa Miriam Balderas Ishwarlal Jialal Xinpu Chen Ruth Ann Luna Sridevi Devaraj |
| author_sort | Roma Pahwa |
| collection | DOAJ |
| description | Aims. Diabetes is a proinflammatory state, evidenced by increased pattern recognition receptors and the inflammasome (NOD-like receptor family pyrin domain (NLRP)) complex. Recent reports have elucidated the role of the gut microbiome in diabetes, but there is limited data on the gut microbiome in NLRP-KO mice and its effect on diabetes-induced inflammation. Methods. Gut microbiome composition and biomarkers of inflammation (IL-18, serum amyloid A) were assessed in streptozotocin- (STZ-) induced diabetic mice on a NLRP3-knockout (KO) background versus wild-type diabetic mice. Results. SAA and IL-18 levels were significantly elevated in diabetic mice (STZ) compared to control (WT) mice, and there was a significant attenuation of inflammation in diabetic NLRP3-KO mice (NLRP3-KO STZ) compared to control mice (p<0.005). Principal coordinate analysis clearly separated controls, STZ, and NLRP3-KO STZ mice. Among the different phyla, there was a significant increase in the Firmicutes : Bacteroidetes ratio in the diabetic group compared to controls. When compared to the WT STZ group, the NLRP3-KO STZ group showed a significant decrease in the Firmicutes : Bacteroidetes ratio. Together, these findings indicate that interaction of the intestinal microbes with the innate immune system is a crucial factor that could modify diabetes and complications. |
| format | Article |
| id | doaj-art-5274a7f7ac444caebf8dffcc9697a3ef |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-5274a7f7ac444caebf8dffcc9697a3ef2025-02-03T05:59:07ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/65197856519785Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout MiceRoma Pahwa0Miriam Balderas1Ishwarlal Jialal2Xinpu Chen3Ruth Ann Luna4Sridevi Devaraj5Veterans Affairs Medical Center, Mather, CA, USATexas Children’s Microbiome Center, Texas Children’s Hospital, Houston, TX, USAVeterans Affairs Medical Center, Mather, CA, USATexas Children’s Microbiome Center, Texas Children’s Hospital, Houston, TX, USATexas Children’s Microbiome Center, Texas Children’s Hospital, Houston, TX, USATexas Children’s Microbiome Center, Texas Children’s Hospital, Houston, TX, USAAims. Diabetes is a proinflammatory state, evidenced by increased pattern recognition receptors and the inflammasome (NOD-like receptor family pyrin domain (NLRP)) complex. Recent reports have elucidated the role of the gut microbiome in diabetes, but there is limited data on the gut microbiome in NLRP-KO mice and its effect on diabetes-induced inflammation. Methods. Gut microbiome composition and biomarkers of inflammation (IL-18, serum amyloid A) were assessed in streptozotocin- (STZ-) induced diabetic mice on a NLRP3-knockout (KO) background versus wild-type diabetic mice. Results. SAA and IL-18 levels were significantly elevated in diabetic mice (STZ) compared to control (WT) mice, and there was a significant attenuation of inflammation in diabetic NLRP3-KO mice (NLRP3-KO STZ) compared to control mice (p<0.005). Principal coordinate analysis clearly separated controls, STZ, and NLRP3-KO STZ mice. Among the different phyla, there was a significant increase in the Firmicutes : Bacteroidetes ratio in the diabetic group compared to controls. When compared to the WT STZ group, the NLRP3-KO STZ group showed a significant decrease in the Firmicutes : Bacteroidetes ratio. Together, these findings indicate that interaction of the intestinal microbes with the innate immune system is a crucial factor that could modify diabetes and complications.http://dx.doi.org/10.1155/2017/6519785 |
| spellingShingle | Roma Pahwa Miriam Balderas Ishwarlal Jialal Xinpu Chen Ruth Ann Luna Sridevi Devaraj Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice Journal of Diabetes Research |
| title | Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice |
| title_full | Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice |
| title_fullStr | Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice |
| title_full_unstemmed | Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice |
| title_short | Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice |
| title_sort | gut microbiome and inflammation a study of diabetic inflammasome knockout mice |
| url | http://dx.doi.org/10.1155/2017/6519785 |
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