Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sprouting
Abstract Sprouting ability is an important indicator for evaluating the production performance of sweetpotato. However, the storage roots sprouting ability varies among different varieties. Currently, research on the sweetpotato storage roots sprouting (SPSRS) mainly focuses on physiology, rarely st...
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BMC
2025-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-07030-7 |
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| author | Fan Ding Xue Zou Heling Fan Li-ping Rao Jian Sun Qiang Li |
| author_facet | Fan Ding Xue Zou Heling Fan Li-ping Rao Jian Sun Qiang Li |
| author_sort | Fan Ding |
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| description | Abstract Sprouting ability is an important indicator for evaluating the production performance of sweetpotato. However, the storage roots sprouting ability varies among different varieties. Currently, research on the sweetpotato storage roots sprouting (SPSRS) mainly focuses on physiology, rarely studies on the key metabolism and regulatory genes during the sprouting process. In this study, we compared transcriptomes and hormone metabolites in sprout buds of two sweetpotato varieties with contrasting sprouting abilities (strong-sprouting vs. late-sprouting) at 0 and 3 days post-harvest. In the SPSRS process of strong-sprouting variety (0d_vs_3d), a total of 2902 differentially expressed genes (DEGs), including 2411 up-regulated and 491 down-regulated genes were identified. But they were detected only, 45 up-regulated genes and 126 down-regulated genes between 0d and 3d of late-sprouting variety. Gene Ontology (GO) analysis showed that the DEGs between 0d and 3d in strong-sprouting variety and late-sprouting variety were mainly related to photosynthesis, light harvesting (GO:0009765), photosystem II (GO:0009523), and chlorophyll-binding (GO:0016168). In the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, DEGs in strong-sprouting variety were mainly associated with photosynthesis-antenna proteins. Both GO analysis and KEGG analysis indicated that DEGs in strong-sprouting variety (0d_vs_3d) were primarily related to photosynthesis. Hormone metabolites detection results showed that low abscisic acid (ABA) concentrations are crucial for SPSRS, while high indole-3-acetic acid (IAA) concentrations play a promoting role. Gibberellins (GAs) and cytokinins (CKs) are not key hormones for SPSRS. During the SPSRS process, IbGA2ox, IbGA20ox, and IbGA3ox were involved in regulation of GAs, while IbAMI1 was involved in regulation of IAA, and IbCYP707A1 and IbUGT73B2 were involved in regulation of ABA. Our study results also suggest that IbCYP707A1 is a key gene for SPSRS. |
| format | Article |
| id | doaj-art-526a75df9ecc4bf19ea5ef35c3f46d2f |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-526a75df9ecc4bf19ea5ef35c3f46d2f2025-08-20T03:45:56ZengBMCBMC Plant Biology1471-22292025-08-0125111410.1186/s12870-025-07030-7Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sproutingFan Ding0Xue Zou1Heling Fan2Li-ping Rao3Jian Sun4Qiang Li5School of Life Sciences, Jiangsu Normal UniversityCrop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang Academy of Agricultural SciencesCrop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang Academy of Agricultural SciencesCrop Characteristic Resources Creation and Utilization Key Laboratory of Sichuan Province, Mianyang Academy of Agricultural SciencesSchool of Life Sciences, Jiangsu Normal UniversitySchool of Life Sciences, Jiangsu Normal UniversityAbstract Sprouting ability is an important indicator for evaluating the production performance of sweetpotato. However, the storage roots sprouting ability varies among different varieties. Currently, research on the sweetpotato storage roots sprouting (SPSRS) mainly focuses on physiology, rarely studies on the key metabolism and regulatory genes during the sprouting process. In this study, we compared transcriptomes and hormone metabolites in sprout buds of two sweetpotato varieties with contrasting sprouting abilities (strong-sprouting vs. late-sprouting) at 0 and 3 days post-harvest. In the SPSRS process of strong-sprouting variety (0d_vs_3d), a total of 2902 differentially expressed genes (DEGs), including 2411 up-regulated and 491 down-regulated genes were identified. But they were detected only, 45 up-regulated genes and 126 down-regulated genes between 0d and 3d of late-sprouting variety. Gene Ontology (GO) analysis showed that the DEGs between 0d and 3d in strong-sprouting variety and late-sprouting variety were mainly related to photosynthesis, light harvesting (GO:0009765), photosystem II (GO:0009523), and chlorophyll-binding (GO:0016168). In the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, DEGs in strong-sprouting variety were mainly associated with photosynthesis-antenna proteins. Both GO analysis and KEGG analysis indicated that DEGs in strong-sprouting variety (0d_vs_3d) were primarily related to photosynthesis. Hormone metabolites detection results showed that low abscisic acid (ABA) concentrations are crucial for SPSRS, while high indole-3-acetic acid (IAA) concentrations play a promoting role. Gibberellins (GAs) and cytokinins (CKs) are not key hormones for SPSRS. During the SPSRS process, IbGA2ox, IbGA20ox, and IbGA3ox were involved in regulation of GAs, while IbAMI1 was involved in regulation of IAA, and IbCYP707A1 and IbUGT73B2 were involved in regulation of ABA. Our study results also suggest that IbCYP707A1 is a key gene for SPSRS.https://doi.org/10.1186/s12870-025-07030-7SweetpotatoStorage roots sproutingTranscriptomeHormones metabolite |
| spellingShingle | Fan Ding Xue Zou Heling Fan Li-ping Rao Jian Sun Qiang Li Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sprouting BMC Plant Biology Sweetpotato Storage roots sprouting Transcriptome Hormones metabolite |
| title | Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sprouting |
| title_full | Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sprouting |
| title_fullStr | Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sprouting |
| title_full_unstemmed | Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sprouting |
| title_short | Transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of Sweetpotato storage roots sprouting |
| title_sort | transcriptome and hormones metabolome joint analyses reveal the key molecular mechanism of sweetpotato storage roots sprouting |
| topic | Sweetpotato Storage roots sprouting Transcriptome Hormones metabolite |
| url | https://doi.org/10.1186/s12870-025-07030-7 |
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