Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?
Cytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/890706 |
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| _version_ | 1850224213791604736 |
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| author | Luke Parkitny James H. McAuley Patrick J. Kelly Flavia Di Pietro Barbara Cameron G. Lorimer Moseley |
| author_facet | Luke Parkitny James H. McAuley Patrick J. Kelly Flavia Di Pietro Barbara Cameron G. Lorimer Moseley |
| author_sort | Luke Parkitny |
| collection | DOAJ |
| description | Cytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between concentrations of cytokines in plasma and serum, except for MIP1β. A thaw condition significantly changed the concentrations of most cytokines, but serum appeared less affected by this than plasma was. Closer examination using Bland-Altman analyses revealed that for each comparison, agreement was moderately good for many cytokine concentrations. This is important because measures of agreement must be interpreted based on the required precision, which may differ between clinical and research demands. We also identified that for some cytokines, the relationship between serum and plasma is affected by concentration, thus advocating for the use of appropriate methods when performing such comparisons in studies such as systematic reviews and meta-analyses. |
| format | Article |
| id | doaj-art-5259145398fe4ff4af1f7752a83d55b7 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5259145398fe4ff4af1f7752a83d55b72025-08-20T02:05:42ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/890706890706Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter?Luke Parkitny0James H. McAuley1Patrick J. Kelly2Flavia Di Pietro3Barbara Cameron4G. Lorimer Moseley5Neuroscience Research Australia, Barker Street, Randwick, NSW 2031, AustraliaNeuroscience Research Australia, Barker Street, Randwick, NSW 2031, AustraliaSydney School of Public Health, The University of Sydney, Edward Ford Building A27, Sydney, NSW 2006, AustraliaNeuroscience Research Australia, Barker Street, Randwick, NSW 2031, AustraliaCentre for Infection and Inflammation Research, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, AustraliaNeuroscience Research Australia, Barker Street, Randwick, NSW 2031, AustraliaCytokine concentrations are thought to be affected by methods of sampling and processing and by storage conditions. In this study we compared 17 cytokine concentrations obtained from plasma and serum at baseline and after a controlled thaw condition. We found that absolute agreement was poor between concentrations of cytokines in plasma and serum, except for MIP1β. A thaw condition significantly changed the concentrations of most cytokines, but serum appeared less affected by this than plasma was. Closer examination using Bland-Altman analyses revealed that for each comparison, agreement was moderately good for many cytokine concentrations. This is important because measures of agreement must be interpreted based on the required precision, which may differ between clinical and research demands. We also identified that for some cytokines, the relationship between serum and plasma is affected by concentration, thus advocating for the use of appropriate methods when performing such comparisons in studies such as systematic reviews and meta-analyses.http://dx.doi.org/10.1155/2013/890706 |
| spellingShingle | Luke Parkitny James H. McAuley Patrick J. Kelly Flavia Di Pietro Barbara Cameron G. Lorimer Moseley Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter? Mediators of Inflammation |
| title | Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter? |
| title_full | Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter? |
| title_fullStr | Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter? |
| title_full_unstemmed | Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter? |
| title_short | Multiplex Cytokine Concentration Measurement: How Much Do the Medium and Handling Matter? |
| title_sort | multiplex cytokine concentration measurement how much do the medium and handling matter |
| url | http://dx.doi.org/10.1155/2013/890706 |
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