Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum Protein
An 8-week growth experiment was conducted to investigate effects of tributyrin (TB) supplementation on growth performance, intestinal digestive enzyme activity, antioxidant capacity, and inflammation-related gene expression of juvenile large yellow croaker (Larimichthys crocea) (initial weight of 12...
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Wiley
2023-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2023/2687734 |
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| author | Xiuneng Wang Min Wan Zhen Wang Haitao Zhang Si Zhu Xiufei Cao Ning Xu Jichang Zheng Xianyong Bu Wei Xu Kangsen Mai Qinghui Ai |
| author_facet | Xiuneng Wang Min Wan Zhen Wang Haitao Zhang Si Zhu Xiufei Cao Ning Xu Jichang Zheng Xianyong Bu Wei Xu Kangsen Mai Qinghui Ai |
| author_sort | Xiuneng Wang |
| collection | DOAJ |
| description | An 8-week growth experiment was conducted to investigate effects of tributyrin (TB) supplementation on growth performance, intestinal digestive enzyme activity, antioxidant capacity, and inflammation-related gene expression of juvenile large yellow croaker (Larimichthys crocea) (initial weight of 12.90±0.02 g) fed diets with high level of Clostridium autoethanogenum protein (CAP). In the negative control diet, 40% fish meal was used as the major source of protein (named as FM), while 45% fish meal protein of FM was substituted with CAP (named as FC) to form a positive control diet. Based on the FC diet, grade levels of 0.05%, 0.1%, 0.2%, 0.4%, and 0.8% tributyrin were added to formulate other five experimental diets. Results showed that fish fed diets with high levels of CAP significantly decreased the weight gain rate (WGR) and specific growth rate (SGR) compared with fish fed the FM diet (P<0.05). WGR and SGR were significantly higher than in fish fed diets with 0.05% and 0.1% tributyrin that fed the FC diet (P<0.05). Supplementation of 0.1% tributyrin significantly elevated fish intestinal lipase and protease activities compared to FM and FC diets (P<0.05). Meanwhile, compared to fish fed the FC diet, fish fed diets with 0.05% and 0.1% tributyrin showed remarkably higher intestinal total antioxidant capacity (T-AOC). Malondialdehyde (MDA) content in the intestine of fish fed diets with 0.05%-0.4% tributyrin was remarkably lower than those in the fish fed the FC diet (P<0.05). The mRNA expressions of tumor necrosis factor α (tnfα), interleukin-1β (il-1β), interleukin-6 (il-6), and interferon γ (ifnγ) were significantly downregulated in fish fed diets with 0.05%-0.2% tributyrin, and the mRNA expression of il-10 was significantly upregulated in fish fed the 0.2% tributyrin diet (P<0.05). In regard to antioxidant genes, as the supplementation of tributyrin increased from 0.05% to 0.8%, the mRNA expression of nuclear factor erythroid 2-related factor 2 (nrf2) demonstrated a trend of first rising and then decreasing. However, the mRNA expression of Kelch-like ECH-associated protein 1 (keap1) was remarkably lower in fish fed the FC diet than that fed diets with tributyrin supplementation (P<0.05). Overall, fish fed tributyrin supplementation diets can ameliorate the negative effects induced by high proportion of CAP in diets, with an appropriate supplementation of 0.1%. |
| format | Article |
| id | doaj-art-5251a1b384cb46e88eff13e52355a319 |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2023-01-01 |
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| series | Aquaculture Nutrition |
| spelling | doaj-art-5251a1b384cb46e88eff13e52355a3192025-02-03T06:04:52ZengWileyAquaculture Nutrition1365-20952023-01-01202310.1155/2023/2687734Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum ProteinXiuneng Wang0Min Wan1Zhen Wang2Haitao Zhang3Si Zhu4Xiufei Cao5Ning Xu6Jichang Zheng7Xianyong Bu8Wei Xu9Kangsen Mai10Qinghui Ai11Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Guangdong Evergreen Feed Industry Co.Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affair) and Key Laboratory of Mariculture (Ministry of Education)An 8-week growth experiment was conducted to investigate effects of tributyrin (TB) supplementation on growth performance, intestinal digestive enzyme activity, antioxidant capacity, and inflammation-related gene expression of juvenile large yellow croaker (Larimichthys crocea) (initial weight of 12.90±0.02 g) fed diets with high level of Clostridium autoethanogenum protein (CAP). In the negative control diet, 40% fish meal was used as the major source of protein (named as FM), while 45% fish meal protein of FM was substituted with CAP (named as FC) to form a positive control diet. Based on the FC diet, grade levels of 0.05%, 0.1%, 0.2%, 0.4%, and 0.8% tributyrin were added to formulate other five experimental diets. Results showed that fish fed diets with high levels of CAP significantly decreased the weight gain rate (WGR) and specific growth rate (SGR) compared with fish fed the FM diet (P<0.05). WGR and SGR were significantly higher than in fish fed diets with 0.05% and 0.1% tributyrin that fed the FC diet (P<0.05). Supplementation of 0.1% tributyrin significantly elevated fish intestinal lipase and protease activities compared to FM and FC diets (P<0.05). Meanwhile, compared to fish fed the FC diet, fish fed diets with 0.05% and 0.1% tributyrin showed remarkably higher intestinal total antioxidant capacity (T-AOC). Malondialdehyde (MDA) content in the intestine of fish fed diets with 0.05%-0.4% tributyrin was remarkably lower than those in the fish fed the FC diet (P<0.05). The mRNA expressions of tumor necrosis factor α (tnfα), interleukin-1β (il-1β), interleukin-6 (il-6), and interferon γ (ifnγ) were significantly downregulated in fish fed diets with 0.05%-0.2% tributyrin, and the mRNA expression of il-10 was significantly upregulated in fish fed the 0.2% tributyrin diet (P<0.05). In regard to antioxidant genes, as the supplementation of tributyrin increased from 0.05% to 0.8%, the mRNA expression of nuclear factor erythroid 2-related factor 2 (nrf2) demonstrated a trend of first rising and then decreasing. However, the mRNA expression of Kelch-like ECH-associated protein 1 (keap1) was remarkably lower in fish fed the FC diet than that fed diets with tributyrin supplementation (P<0.05). Overall, fish fed tributyrin supplementation diets can ameliorate the negative effects induced by high proportion of CAP in diets, with an appropriate supplementation of 0.1%.http://dx.doi.org/10.1155/2023/2687734 |
| spellingShingle | Xiuneng Wang Min Wan Zhen Wang Haitao Zhang Si Zhu Xiufei Cao Ning Xu Jichang Zheng Xianyong Bu Wei Xu Kangsen Mai Qinghui Ai Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum Protein Aquaculture Nutrition |
| title | Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum Protein |
| title_full | Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum Protein |
| title_fullStr | Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum Protein |
| title_full_unstemmed | Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum Protein |
| title_short | Effects of Tributyrin Supplementation on Growth Performance, Intestinal Digestive Enzyme Activity, Antioxidant Capacity, and Inflammation-Related Gene Expression of Large Yellow Croaker (Larimichthys crocea) Fed with a High Level of Clostridium autoethanogenum Protein |
| title_sort | effects of tributyrin supplementation on growth performance intestinal digestive enzyme activity antioxidant capacity and inflammation related gene expression of large yellow croaker larimichthys crocea fed with a high level of clostridium autoethanogenum protein |
| url | http://dx.doi.org/10.1155/2023/2687734 |
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