The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations
Although the use of cryoprotectant dimethyl sulfoxide (DMSO) is the gold standard in cryopreservation of hematopoietic stem cells, it is well known that it has a negative effect on cell viability. The aim of this prospective study was to examine how the length of post-thaw exposure to DMSO affects t...
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Sciendo
2023-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2023-0037 |
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| author | Rimac Vladimira Bojanić Ines Dabelić Sanja Ćepulić Branka Golubić |
| author_facet | Rimac Vladimira Bojanić Ines Dabelić Sanja Ćepulić Branka Golubić |
| author_sort | Rimac Vladimira |
| collection | DOAJ |
| description | Although the use of cryoprotectant dimethyl sulfoxide (DMSO) is the gold standard in cryopreservation of hematopoietic stem cells, it is well known that it has a negative effect on cell viability. The aim of this prospective study was to examine how the length of post-thaw exposure to DMSO affects the cell viability and stability of peripheral blood stem cell (PBSC) samples. Additionally, the effects of donor type and pre-cryopreservation storage time on post-thaw viability during the stability study were evaluated. In 30 autologous and 30 allogeneic PBSC samples viable CD34+, CD14+, CD19+, CD16+/56+, and CD3+ cells were determined immediately after thawing, and one-and three-hours post-thaw. |
| format | Article |
| id | doaj-art-524fbe78656d4014a5542f3428615f1c |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Sciendo |
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| series | Acta Pharmaceutica |
| spelling | doaj-art-524fbe78656d4014a5542f3428615f1c2025-02-03T00:57:30ZengSciendoActa Pharmaceutica1846-95582023-12-0173465567210.2478/acph-2023-0037The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulationsRimac Vladimira0Bojanić Ines1Dabelić Sanja2Ćepulić Branka Golubić31Department of Transfusion Medicine and Transplantation, Biology University Hospital Centre Zagreb10000Zagreb, Croatia1Department of Transfusion Medicine and Transplantation, Biology University Hospital Centre Zagreb10000Zagreb, Croatia2University of ZagrebFaculty of Pharmacy and Biochemistry, 10000Zagreb, Croatia3University of Zagreb School of Medicine, 10000Zagreb, CroatiaAlthough the use of cryoprotectant dimethyl sulfoxide (DMSO) is the gold standard in cryopreservation of hematopoietic stem cells, it is well known that it has a negative effect on cell viability. The aim of this prospective study was to examine how the length of post-thaw exposure to DMSO affects the cell viability and stability of peripheral blood stem cell (PBSC) samples. Additionally, the effects of donor type and pre-cryopreservation storage time on post-thaw viability during the stability study were evaluated. In 30 autologous and 30 allogeneic PBSC samples viable CD34+, CD14+, CD19+, CD16+/56+, and CD3+ cells were determined immediately after thawing, and one-and three-hours post-thaw.https://doi.org/10.2478/acph-2023-0037cryopreservationsample stabilityviabilityperipheral blood stem cells |
| spellingShingle | Rimac Vladimira Bojanić Ines Dabelić Sanja Ćepulić Branka Golubić The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations Acta Pharmaceutica cryopreservation sample stability viability peripheral blood stem cells |
| title | The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations |
| title_full | The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations |
| title_fullStr | The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations |
| title_full_unstemmed | The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations |
| title_short | The impact of cryoprotectant exposure time on post-thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations |
| title_sort | impact of cryoprotectant exposure time on post thaw viability of autologous and allogeneic hematopoietic stem cells and leukocyte subpopulations |
| topic | cryopreservation sample stability viability peripheral blood stem cells |
| url | https://doi.org/10.2478/acph-2023-0037 |
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