High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells
The spatial organization of the nucleus is a key determinant in all genome activities. However, the accurate measurement of the nuclear organization is still technically challenging. Here, the technology NucQuant we created previously was utilized to detect the variation of the nuclear organization,...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/9951114 |
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| _version_ | 1832563633449598976 |
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| author | Renjie Wang Aiwen Huang Yan Wang Pengxin Mei He Zhu Qianqian Chen Sankui Xu |
| author_facet | Renjie Wang Aiwen Huang Yan Wang Pengxin Mei He Zhu Qianqian Chen Sankui Xu |
| author_sort | Renjie Wang |
| collection | DOAJ |
| description | The spatial organization of the nucleus is a key determinant in all genome activities. However, the accurate measurement of the nuclear organization is still technically challenging. Here, the technology NucQuant we created previously was utilized to detect the variation of the nuclear organization, including the heterogeneity of the nuclear geometry, the change of the NPC distribution along different cell cycle stages during interphase, and the organization of the nucleolus. The results confirmed that not only the growth rate and the NPC distribution are influenced by the carbon source; the nuclear shape is also impacted by the carbon source. The nuclei lost their spherical geometry gradually when the cell was cultured from the most to a less favorable carbon source. We also discovered that the nucleolus prefers to locate at the nuclear periphery, which was called the “genes poor region,” especially when the cells entered quiescence. Furthermore, the distribution of the NPC along the different stages during the interphase was analyzed. We proposed that with the growth of the cell, the nucleus would grow from the surface of the NE flanking the nucleolus firstly. |
| format | Article |
| id | doaj-art-523aab99825a4c2eb7bcc67c9f5e5faa |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-523aab99825a4c2eb7bcc67c9f5e5faa2025-02-03T01:12:54ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/99511149951114High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast CellsRenjie Wang0Aiwen Huang1Yan Wang2Pengxin Mei3He Zhu4Qianqian Chen5Sankui Xu6College of Materials Science & Engineering, Henan University of Technology, Zhengzhou, ChinaCollege of Materials Science & Engineering, Henan University of Technology, Zhengzhou, ChinaCollege of Materials Science & Engineering, Henan University of Technology, Zhengzhou, ChinaCollege of Materials Science & Engineering, Henan University of Technology, Zhengzhou, ChinaCollege of Materials Science & Engineering, Henan University of Technology, Zhengzhou, ChinaCollege of Materials Science & Engineering, Henan University of Technology, Zhengzhou, ChinaCollege of Materials Science & Engineering, Henan University of Technology, Zhengzhou, ChinaThe spatial organization of the nucleus is a key determinant in all genome activities. However, the accurate measurement of the nuclear organization is still technically challenging. Here, the technology NucQuant we created previously was utilized to detect the variation of the nuclear organization, including the heterogeneity of the nuclear geometry, the change of the NPC distribution along different cell cycle stages during interphase, and the organization of the nucleolus. The results confirmed that not only the growth rate and the NPC distribution are influenced by the carbon source; the nuclear shape is also impacted by the carbon source. The nuclei lost their spherical geometry gradually when the cell was cultured from the most to a less favorable carbon source. We also discovered that the nucleolus prefers to locate at the nuclear periphery, which was called the “genes poor region,” especially when the cells entered quiescence. Furthermore, the distribution of the NPC along the different stages during the interphase was analyzed. We proposed that with the growth of the cell, the nucleus would grow from the surface of the NE flanking the nucleolus firstly.http://dx.doi.org/10.1155/2021/9951114 |
| spellingShingle | Renjie Wang Aiwen Huang Yan Wang Pengxin Mei He Zhu Qianqian Chen Sankui Xu High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells Stem Cells International |
| title | High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells |
| title_full | High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells |
| title_fullStr | High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells |
| title_full_unstemmed | High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells |
| title_short | High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells |
| title_sort | high resolution microscopy to learn the nuclear organization of the living yeast cells |
| url | http://dx.doi.org/10.1155/2021/9951114 |
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