Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep
The purpose of this study was to investigate the polymorphism and genetic correlation of INSL3 and WNT2B genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of INSL3 and WNT2B genes were analyzed, and their association with litter size and estro...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2025.2498677 |
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| author | Jingdong Bi Sulaiman Yiming Jiaqi Li Quanfeng Wang Manjun Zhai Shaoqi Cao Mengting Zhu Hua Yang |
| author_facet | Jingdong Bi Sulaiman Yiming Jiaqi Li Quanfeng Wang Manjun Zhai Shaoqi Cao Mengting Zhu Hua Yang |
| author_sort | Jingdong Bi |
| collection | DOAJ |
| description | The purpose of this study was to investigate the polymorphism and genetic correlation of INSL3 and WNT2B genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of INSL3 and WNT2B genes were analyzed, and their association with litter size and estrous traits were analyzed. The results showed that two SNPs (SNP1, SNP2) were detected in INSL3 gene and there were three genotypes in SNP2 (INSL3 (A100T)), named of AA, AT and TT, A was the dominant allele. Additionally, five SNPs (SNP3, SNP4, SNP5, SNP6, SNP7) were detected in the WNT2B gene and there were three genotypes in SNP4 (WNT2B (G126T)), named GG, GT and TT, G was dominant allele. SNP2 was in Hardy-Weinberg equilibrium in three sheep breeds (P > 0.05). SNP4 was deviated from Hardy-Weinberg equilibrium in three sheep breeds (P < 0.05). Further, AT genotype of SNP2 (INSL3 (A100T)) could significantly affect the estrus trait in Duolang sheep and Qira black sheep, and related to the litter size in Duolang sheep. The WNT2B significantly affected the estrus and litter size of Duolang sheep and Qira black sheep. INSL3 (A100T) and WNT2B (G126T) may be potential molecular markers for controlling seasonal reproductive trait in sheep. |
| format | Article |
| id | doaj-art-5231be081a5b47e6bd36ca1c06c7797a |
| institution | OA Journals |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-5231be081a5b47e6bd36ca1c06c7797a2025-08-20T01:48:48ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782025-12-0136110.1080/10495398.2025.2498677Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheepJingdong Bi0Sulaiman Yiming1Jiaqi Li2Quanfeng Wang3Manjun Zhai4Shaoqi Cao5Mengting Zhu6Hua Yang7College of Animal Science, Xinjiang Agricultural University, Urumqi, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi, ChinaXinjiang Jinken Aoqun Agriculture and Animal, Husbandry Technology Co., Ltd., Yutian, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi, ChinaXinjiang Uygur Autonomous Region Animal Husbandry General Station, Urumqi, ChinaCollege of Animal Science, Xinjiang Agricultural University, Urumqi, ChinaState Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, ChinaThe purpose of this study was to investigate the polymorphism and genetic correlation of INSL3 and WNT2B genes with seasonal estrus and litter size in three different Xinjiang sheep breeds. The genetic diversity of INSL3 and WNT2B genes were analyzed, and their association with litter size and estrous traits were analyzed. The results showed that two SNPs (SNP1, SNP2) were detected in INSL3 gene and there were three genotypes in SNP2 (INSL3 (A100T)), named of AA, AT and TT, A was the dominant allele. Additionally, five SNPs (SNP3, SNP4, SNP5, SNP6, SNP7) were detected in the WNT2B gene and there were three genotypes in SNP4 (WNT2B (G126T)), named GG, GT and TT, G was dominant allele. SNP2 was in Hardy-Weinberg equilibrium in three sheep breeds (P > 0.05). SNP4 was deviated from Hardy-Weinberg equilibrium in three sheep breeds (P < 0.05). Further, AT genotype of SNP2 (INSL3 (A100T)) could significantly affect the estrus trait in Duolang sheep and Qira black sheep, and related to the litter size in Duolang sheep. The WNT2B significantly affected the estrus and litter size of Duolang sheep and Qira black sheep. INSL3 (A100T) and WNT2B (G126T) may be potential molecular markers for controlling seasonal reproductive trait in sheep.https://www.tandfonline.com/doi/10.1080/10495398.2025.2498677Sheep (Ovis aries)seasonal reproductionlitter sizeINSL3WNT2B |
| spellingShingle | Jingdong Bi Sulaiman Yiming Jiaqi Li Quanfeng Wang Manjun Zhai Shaoqi Cao Mengting Zhu Hua Yang Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep Animal Biotechnology Sheep (Ovis aries) seasonal reproduction litter size INSL3 WNT2B |
| title | Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep |
| title_full | Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep |
| title_fullStr | Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep |
| title_full_unstemmed | Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep |
| title_short | Effects of INSL3 and WNT2B gene polymorphisms on seasonal reproductive traits of Xinjiang Qira black sheep, Kazakh sheep and Duolang sheep |
| title_sort | effects of insl3 and wnt2b gene polymorphisms on seasonal reproductive traits of xinjiang qira black sheep kazakh sheep and duolang sheep |
| topic | Sheep (Ovis aries) seasonal reproduction litter size INSL3 WNT2B |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2025.2498677 |
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