Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MS
Proteomics analysis is crucial for understanding the molecular mechanisms underlying muscle adaptations to different types of exercise, such as concentric and eccentric training. Traditional methods like two-dimensional gel electrophoresis and standard mass spectrometry have been used to analyze mus...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2024-12-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5137&type=0 |
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| author | Jiawei Du Jinghua Hou Hezhang Yun Yafeng Song |
| author_facet | Jiawei Du Jinghua Hou Hezhang Yun Yafeng Song |
| author_sort | Jiawei Du |
| collection | DOAJ |
| description | Proteomics analysis is crucial for understanding the molecular mechanisms underlying muscle adaptations to different types of exercise, such as concentric and eccentric training. Traditional methods like two-dimensional gel electrophoresis and standard mass spectrometry have been used to analyze muscle protein content and modifications. This protocol details the preparation of muscle samples for proteomics analysis using ultra-high-performance liquid chromatography (UHPLC). It includes steps for muscle biopsy collection, protein extraction, digestion, and UHPLC-based analysis. The UHPLC method offers high-resolution separation of complex protein mixtures, providing more detailed and accurate proteomic profiles compared to conventional techniques. This protocol significantly enhances sensitivity, reproducibility, and efficiency, making it ideal for comprehensive muscle proteomics studies. |
| format | Article |
| id | doaj-art-5230e9003dbd46bf8819c91a5fd0a5ca |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-5230e9003dbd46bf8819c91a5fd0a5ca2025-02-07T08:16:22ZengBio-protocol LLCBio-Protocol2331-83252024-12-01142410.21769/BioProtoc.5137Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MSJiawei Du0Jinghua Hou1Hezhang Yun2Yafeng Song3Key Laboratory of Sports and Physical Fitness of the Ministry of Education, Beijing Sport University, Beijing, ChinaKey Laboratory of Sports and Physical Fitness of the Ministry of Education, Beijing Sport University, Beijing, ChinaKey Laboratory of Sports and Physical Fitness of the Ministry of Education, Beijing Sport University, Beijing, ChinaKey Laboratory of Sports and Physical Fitness of the Ministry of Education, Beijing Sport University, Beijing, ChinaProteomics analysis is crucial for understanding the molecular mechanisms underlying muscle adaptations to different types of exercise, such as concentric and eccentric training. Traditional methods like two-dimensional gel electrophoresis and standard mass spectrometry have been used to analyze muscle protein content and modifications. This protocol details the preparation of muscle samples for proteomics analysis using ultra-high-performance liquid chromatography (UHPLC). It includes steps for muscle biopsy collection, protein extraction, digestion, and UHPLC-based analysis. The UHPLC method offers high-resolution separation of complex protein mixtures, providing more detailed and accurate proteomic profiles compared to conventional techniques. This protocol significantly enhances sensitivity, reproducibility, and efficiency, making it ideal for comprehensive muscle proteomics studies.https://bio-protocol.org/en/bpdetail?id=5137&type=0 |
| spellingShingle | Jiawei Du Jinghua Hou Hezhang Yun Yafeng Song Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MS Bio-Protocol |
| title | Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MS |
| title_full | Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MS |
| title_fullStr | Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MS |
| title_full_unstemmed | Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MS |
| title_short | Muscle Biopsy Sample Preparation and Proteomics Analysis Based on UHPLC-MS/MS |
| title_sort | muscle biopsy sample preparation and proteomics analysis based on uhplc ms ms |
| url | https://bio-protocol.org/en/bpdetail?id=5137&type=0 |
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