Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation
Background: Suppressor of cytokine signaling (SOCS)3 is a regulatory protein that participates in an important negative feedback loop downstream of several critical cytokines, especially members of the interleukin-6 (IL-6) family. As a result, SOCS3 has been shown to impact the de...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IMR Press
2025-04-01
|
| Series: | Frontiers in Bioscience-Landmark |
| Subjects: | |
| Online Access: | https://www.imrpress.com/journal/FBL/30/4/10.31083/FBL36537 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849310727894990848 |
|---|---|
| author | Mohamed Luban Sobah Clifford Liongue Alister C. Ward |
| author_facet | Mohamed Luban Sobah Clifford Liongue Alister C. Ward |
| author_sort | Mohamed Luban Sobah |
| collection | DOAJ |
| description | Background: Suppressor of cytokine signaling (SOCS)3 is a regulatory protein that participates in an important negative feedback loop downstream of several critical cytokines, especially members of the interleukin-6 (IL-6) family. As a result, SOCS3 has been shown to impact the development and function of blood and immune cells. Zebrafish harbor duplicates of SOCS3, Socs3a and Socs3b, both of which possess conserved functional domains. Methods: This study explored the role of zebrafish Socs3a by creating a whole genome knockout using CRISPR/Cas9, with a focus on hematopoiesis and neuromast formation. Results: A zebrafish Socs3a knockout mutant was successfully generated. Characterization of this mutant revealed that normal hematopoiesis was not impacted nor was neutrophils lacking Socs3a displayed normal responses to injury or their production during emergency granulopoiesis. Neuromast formation was severely impacted in Socs3a knockout zebrafish. Conclusions: Zebrafish Socs3a mutants display normal hematopoiesis and myeloid function, but the formation of the lateral line neuromast was affected by the absence of Socs3a. |
| format | Article |
| id | doaj-art-521d8afdffc548dd913573c87da7e15e |
| institution | Kabale University |
| issn | 2768-6701 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj-art-521d8afdffc548dd913573c87da7e15e2025-08-20T03:53:39ZengIMR PressFrontiers in Bioscience-Landmark2768-67012025-04-013043653710.31083/FBL36537S2768-6701(25)01686-7Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast FormationMohamed Luban Sobah0Clifford Liongue1Alister C. Ward2School of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaBackground: Suppressor of cytokine signaling (SOCS)3 is a regulatory protein that participates in an important negative feedback loop downstream of several critical cytokines, especially members of the interleukin-6 (IL-6) family. As a result, SOCS3 has been shown to impact the development and function of blood and immune cells. Zebrafish harbor duplicates of SOCS3, Socs3a and Socs3b, both of which possess conserved functional domains. Methods: This study explored the role of zebrafish Socs3a by creating a whole genome knockout using CRISPR/Cas9, with a focus on hematopoiesis and neuromast formation. Results: A zebrafish Socs3a knockout mutant was successfully generated. Characterization of this mutant revealed that normal hematopoiesis was not impacted nor was neutrophils lacking Socs3a displayed normal responses to injury or their production during emergency granulopoiesis. Neuromast formation was severely impacted in Socs3a knockout zebrafish. Conclusions: Zebrafish Socs3a mutants display normal hematopoiesis and myeloid function, but the formation of the lateral line neuromast was affected by the absence of Socs3a.https://www.imrpress.com/journal/FBL/30/4/10.31083/FBL36537cytokinesuppressor of cytokine signaling 3 proteinmyelopoiesiszebrafish |
| spellingShingle | Mohamed Luban Sobah Clifford Liongue Alister C. Ward Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation Frontiers in Bioscience-Landmark cytokine suppressor of cytokine signaling 3 protein myelopoiesis zebrafish |
| title | Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation |
| title_full | Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation |
| title_fullStr | Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation |
| title_full_unstemmed | Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation |
| title_short | Socs3a is Dispensable for Zebrafish Hematopoiesis and is Required for Neuromast Formation |
| title_sort | socs3a is dispensable for zebrafish hematopoiesis and is required for neuromast formation |
| topic | cytokine suppressor of cytokine signaling 3 protein myelopoiesis zebrafish |
| url | https://www.imrpress.com/journal/FBL/30/4/10.31083/FBL36537 |
| work_keys_str_mv | AT mohamedlubansobah socs3aisdispensableforzebrafishhematopoiesisandisrequiredforneuromastformation AT cliffordliongue socs3aisdispensableforzebrafishhematopoiesisandisrequiredforneuromastformation AT alistercward socs3aisdispensableforzebrafishhematopoiesisandisrequiredforneuromastformation |