Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.)
Plant extracts with insecticidal properties are promising alternatives to conventional insecticides, which may pose risks to human health and the environment in pest management. In the current study, the methanolic extract of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. was c...
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De Gruyter
2024-11-01
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| Series: | Open Agriculture |
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| Online Access: | https://doi.org/10.1515/opag-2022-0394 |
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| author | Khamis Wael Al-Askar Abdulaziz Kowalczewski Przemysław Abdelkhalek Ahmed Behiry Said |
| author_facet | Khamis Wael Al-Askar Abdulaziz Kowalczewski Przemysław Abdelkhalek Ahmed Behiry Said |
| author_sort | Khamis Wael |
| collection | DOAJ |
| description | Plant extracts with insecticidal properties are promising alternatives to conventional insecticides, which may pose risks to human health and the environment in pest management. In the current study, the methanolic extract of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. was characterized using high-performance liquid and gas-mass spectrometry chromatography. High-performance liquid chromatography analysis identified 14 polyphenols, the highest of which is chlorogenic acid (65637.79 µg/g), followed by naringenin and pyrocatechol at 2022.98 and 1509.11 µg/g, respectively. However, quercetin, gallic acid, ferulic acid, rutin, and kaempferol showed moderate quantities. The gas chromatography–mass spectrometry analysis identified the major polysaccharide, inositol (15.52%), followed by squalene and triterpene (11.02%) out of 35 polysaccharides. The major fatty acids, 11-octadecenoic acid, methyl ester, and methyl 9-cis, 11-trans-octadecadienoate, were 9.06 and 6.30%, respectively. The crude extract and chlorfluazuron 5% EC at LC15 were evaluated for the life table and biological aspects of S. littoralis (Boisd.) neonates after 48 h under laboratory conditions compared to the control. The toxicity of the LC15 at 48 h of chlorfluazuron 5% EC (0.31 mg/L) excelled the extract (178.95 mg/L) on the neonates. Life span tests showed that the larval longevity of the extract and chlorfluazuron colonies were 27.34 and 30 days, respectively. The control colony completed its life span at 38.66 days, whereas the extract colony ended at the pupae stage at 33.67 days. Biological aspects showed that the tested compounds decreased pupation percentages and weight. Pupae malformations caused by the extract (80.56%) outperformed chlorfluazuron (33.33%). The adult emergency decreased by the extract (0.33%), and no emergency was observed in chlorfluazuron colony. Ultimately, A. oblongifolia extract demonstrated significant life table elongations and developmental impairment, which may suggest potential and eco-friendly use for its prospective commercial preparations in controlling S. littoralis alternatively to chlorfluazuron 5% EC. |
| format | Article |
| id | doaj-art-521b6238ad4a44f5bcc0336c4283d539 |
| institution | Kabale University |
| issn | 2391-9531 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Open Agriculture |
| spelling | doaj-art-521b6238ad4a44f5bcc0336c4283d5392024-11-20T15:35:35ZengDe GruyterOpen Agriculture2391-95312024-11-01912193410.1515/opag-2022-0394Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.)Khamis Wael0Al-Askar Abdulaziz1Kowalczewski Przemysław2Abdelkhalek Ahmed3Behiry Said4Cotton Pesticides Evaluation Department, Plant Protection Research Institute, Agricultural Research Center, Al-Sabhia, Alexandria, 21616, EgyptDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Food Technology of Plant Origin, Poznań University of Life Sciences, Poznań, PolandPlant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria 21934, EgyptAgricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, EgyptPlant extracts with insecticidal properties are promising alternatives to conventional insecticides, which may pose risks to human health and the environment in pest management. In the current study, the methanolic extract of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. was characterized using high-performance liquid and gas-mass spectrometry chromatography. High-performance liquid chromatography analysis identified 14 polyphenols, the highest of which is chlorogenic acid (65637.79 µg/g), followed by naringenin and pyrocatechol at 2022.98 and 1509.11 µg/g, respectively. However, quercetin, gallic acid, ferulic acid, rutin, and kaempferol showed moderate quantities. The gas chromatography–mass spectrometry analysis identified the major polysaccharide, inositol (15.52%), followed by squalene and triterpene (11.02%) out of 35 polysaccharides. The major fatty acids, 11-octadecenoic acid, methyl ester, and methyl 9-cis, 11-trans-octadecadienoate, were 9.06 and 6.30%, respectively. The crude extract and chlorfluazuron 5% EC at LC15 were evaluated for the life table and biological aspects of S. littoralis (Boisd.) neonates after 48 h under laboratory conditions compared to the control. The toxicity of the LC15 at 48 h of chlorfluazuron 5% EC (0.31 mg/L) excelled the extract (178.95 mg/L) on the neonates. Life span tests showed that the larval longevity of the extract and chlorfluazuron colonies were 27.34 and 30 days, respectively. The control colony completed its life span at 38.66 days, whereas the extract colony ended at the pupae stage at 33.67 days. Biological aspects showed that the tested compounds decreased pupation percentages and weight. Pupae malformations caused by the extract (80.56%) outperformed chlorfluazuron (33.33%). The adult emergency decreased by the extract (0.33%), and no emergency was observed in chlorfluazuron colony. Ultimately, A. oblongifolia extract demonstrated significant life table elongations and developmental impairment, which may suggest potential and eco-friendly use for its prospective commercial preparations in controlling S. littoralis alternatively to chlorfluazuron 5% EC.https://doi.org/10.1515/opag-2022-0394african wintersweethplc–msgc–mscotton leafwormlepidopteranschlorfluazuron |
| spellingShingle | Khamis Wael Al-Askar Abdulaziz Kowalczewski Przemysław Abdelkhalek Ahmed Behiry Said Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.) Open Agriculture african wintersweet hplc–ms gc–ms cotton leafworm lepidopterans chlorfluazuron |
| title | Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.) |
| title_full | Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.) |
| title_fullStr | Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.) |
| title_full_unstemmed | Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.) |
| title_short | Phytochemical composition and insecticidal activity of Acokanthera oblongifolia (Hochst.) Benth & Hook.f. ex B.D.Jacks. extract on life span and biological aspects of Spodoptera littoralis (Biosd.) |
| title_sort | phytochemical composition and insecticidal activity of acokanthera oblongifolia hochst benth hook f ex b d jacks extract on life span and biological aspects of spodoptera littoralis biosd |
| topic | african wintersweet hplc–ms gc–ms cotton leafworm lepidopterans chlorfluazuron |
| url | https://doi.org/10.1515/opag-2022-0394 |
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