The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet
Introduction: Brain-derived neurotrophic factor (BDNF) has beneficial effects on non-neuronal tissues, particularly cardiovascular cells, but its effects on the BDNF-TrkB pathway in heart tissue have not been studied. Therefore, the aim of the study was to investigate the effect of high-intensity i...
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Ilam University of Medical Sciences
2025-05-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/article-1-8411-en.pdf |
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| author | Zainulabden Zainulabden Ali Abdulrahim1 Amir Ghanbarpour Nosrati Mousa Khalafi |
| author_facet | Zainulabden Zainulabden Ali Abdulrahim1 Amir Ghanbarpour Nosrati Mousa Khalafi |
| author_sort | Zainulabden Zainulabden Ali Abdulrahim1 |
| collection | DOAJ |
| description | Introduction: Brain-derived neurotrophic factor (BDNF) has beneficial effects on non-neuronal tissues, particularly cardiovascular cells, but its effects on the BDNF-TrkB pathway in heart tissue have not been studied. Therefore, the aim of the study was to investigate the effect of high-intensity interval training (HIIT) and vitamin D supplementation (VitD) on the protein levels of cardiac BDNF-TrkB in rats fed with a high-fat and high-carbohydrate diet.
Materials & Methods: Sprague Dawley rats were divided into normal diet (ND), high fat and high carbohydrate (HFD+HC), high fat and high carbohydrate with HIIT (HFD+HC+HIIT), high fat and high carbohydrate with VitD (HFD+HC+VitD), and high fat and high carbohydrate with HIIT and VitD (HFD+HC+HII+VitD). Protein levels of BDNF and TrkB were assessed using Western blotting. Independent t-tests, one-way ANOVA, Tukey's test, and SPSS V.26 were used to analyze the data.
Results: Consumption of HFD+HC resulted in a decrease in protein levels of BDNF and TrkB in the heart compared to ND (p<0.05). On the contrary, HIIT and VitD only resulted in a non-significant increase in protein levels of BDNF and TrkB in the heart compared to the HFD+HC group. However, the combination of HIIT and VitD resulted in a significant and non-significant increase in the protein levels of TrkB (P=0.03) and BDNF (P=0.06) compared to the HFD+HC group.
Conclusion: HIIT+VD, by increasing cardiac BDNF and TrkB, may prevent the deleterious effects of high-fat and high-carbohydrate diets on this cardiac signaling, while each of these interventions alone has little effect on cardiac BDNF and TrkB. |
| format | Article |
| id | doaj-art-520d373aca494b9891efec795ab010d6 |
| institution | Kabale University |
| issn | 1563-4728 2588-3135 |
| language | fas |
| publishDate | 2025-05-01 |
| publisher | Ilam University of Medical Sciences |
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| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj-art-520d373aca494b9891efec795ab010d62025-08-25T07:35:08ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352025-05-013328699The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate dietZainulabden Zainulabden Ali Abdulrahim10Amir Ghanbarpour Nosrati1Mousa Khalafi2 Dept of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran Dept of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran Dept of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan, Iran Introduction: Brain-derived neurotrophic factor (BDNF) has beneficial effects on non-neuronal tissues, particularly cardiovascular cells, but its effects on the BDNF-TrkB pathway in heart tissue have not been studied. Therefore, the aim of the study was to investigate the effect of high-intensity interval training (HIIT) and vitamin D supplementation (VitD) on the protein levels of cardiac BDNF-TrkB in rats fed with a high-fat and high-carbohydrate diet. Materials & Methods: Sprague Dawley rats were divided into normal diet (ND), high fat and high carbohydrate (HFD+HC), high fat and high carbohydrate with HIIT (HFD+HC+HIIT), high fat and high carbohydrate with VitD (HFD+HC+VitD), and high fat and high carbohydrate with HIIT and VitD (HFD+HC+HII+VitD). Protein levels of BDNF and TrkB were assessed using Western blotting. Independent t-tests, one-way ANOVA, Tukey's test, and SPSS V.26 were used to analyze the data. Results: Consumption of HFD+HC resulted in a decrease in protein levels of BDNF and TrkB in the heart compared to ND (p<0.05). On the contrary, HIIT and VitD only resulted in a non-significant increase in protein levels of BDNF and TrkB in the heart compared to the HFD+HC group. However, the combination of HIIT and VitD resulted in a significant and non-significant increase in the protein levels of TrkB (P=0.03) and BDNF (P=0.06) compared to the HFD+HC group. Conclusion: HIIT+VD, by increasing cardiac BDNF and TrkB, may prevent the deleterious effects of high-fat and high-carbohydrate diets on this cardiac signaling, while each of these interventions alone has little effect on cardiac BDNF and TrkB.http://sjimu.medilam.ac.ir/article-1-8411-en.pdfbrain-derived neurotrophic factorbdnftropomyosin-related kinase receptor btrkbexercisevitamin d |
| spellingShingle | Zainulabden Zainulabden Ali Abdulrahim1 Amir Ghanbarpour Nosrati Mousa Khalafi The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām brain-derived neurotrophic factor bdnf tropomyosin-related kinase receptor b trkb exercise vitamin d |
| title | The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet |
| title_full | The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet |
| title_fullStr | The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet |
| title_full_unstemmed | The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet |
| title_short | The effect of vitamin D supplementation and high-intensity interval training on BDNF-TrkB expression in rats fed a high-fat and high-carbohydrate diet |
| title_sort | effect of vitamin d supplementation and high intensity interval training on bdnf trkb expression in rats fed a high fat and high carbohydrate diet |
| topic | brain-derived neurotrophic factor bdnf tropomyosin-related kinase receptor b trkb exercise vitamin d |
| url | http://sjimu.medilam.ac.ir/article-1-8411-en.pdf |
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